Duck enteritis virus (DEV) UL54 protein, a novel partner, interacts with DEV UL24 protein

Abstract

BackgroundUL24 is a multifunctional protein that is conserved among alphaherpesviruses and is believed to play an important role in viral infection and replication.ResultsIn this paper, to investigate putative UL24-binding proteins and to explore the functional mechanisms of DEV UL24, yeast two-hybrid (Y2H) was carried out, and further verified the interaction between UL24 and partners by co-immunoprecipitation and fluorescence microscopy experiments. Interaction partners of UL24 protein were screened by yeast two-hybrid (Y2H) with the cDNA library of DEV-CHv strain post-infection DEF cells. A novel partner, DEV UL54 protein, was discovered by Y2H screening and bioinformatic. Co-immunoprecipitation experiments suggested that DEV UL24 interacted with UL54 proteins. And distribution of a part of UL54 protein was changed from nucleus to cytoplasm in DF-1 cells of co-subcellular localization experiments which also showed that DEV UL24 interacted with UL54 proteins.ConclusionsThe interaction between the DEV UL24 and UL54 proteins was discovered for the first time. Thus, DEV UL54 protein as a novel partner interacted with DEV UL24 protein.Electronic supplementary materialThe online version of this article (doi:10.1186/s12985-017-0830-5) contains supplementary material, which is available to authorized users.

Development and application of multiplex PCR method for simultaneous detection of seven viruses in ducks

“…Because of the development of mixed culture models, enhanced mobility of humans and animals, and environment pollution, viral infection in ducks is getting serious in recent years [1]. Among the popular viruses that infected ducks, AIV, NDV, NDPV, DHAV, DTMUV, FAdV, and DEV were very common, seriously endangering ducks’ health and causing great economic losses [5, 7, 10, 11, 28–30]. Although virus isolation is considered to be the gold standard for viral detection, it is unsuitable for clinical rapid detection [31].…”

“…DEV could also cause considerable economic losses to the commercial duck industry due to the high mortality and decreased egg production rate. Therefore, it poses a continuous threat to wild and migratory waterfowl populations [11].…”

Co-localization of and interaction between duck enteritis virus glycoprotein H and L

“…Co-IP is an available and useful approach to test the interaction between two different proteins currently [ 25 ]. Some research has proved the protein-protein interaction through Co-IP assay [ 15 , 37 ], although the quality of specific antibodies are significantly essential [ 25 ]. Basically, massive cellular protein-protein interactions remained, after non-denaturing cell lysis.…”

“…Then, gB, as a fusogen, is activated by gH/gL through interaction, therefore the fusogenic function of gB is promoted [ 23 , 24 , 30 ]. The function of connection between gB and gH/gL, also known as “core fusion machinery,” is essential for herpesviruses to initiate membrane fusion [ 15 ]. For example, the first step of EBV entry into Epithelial cells is tethering gH/gL to its cellular membrane receptors, including integrin αγβ5, αγβ6 and αγβ8, which will change the conformation of gH/gL delicately due to receptor-binding.…”

Duck Enteritis Virus VP16 Antagonizes IFN-β-Mediated Antiviral Innate Immunity

“…The characteristics of some genes from DEV had been reported [45][46][47][48][49][50][51][52][53][54][55][56][57][58][59][60][61][62][63][64]. However, the function of VP16 of duck enteritis virus encoded by the UL48 gene was hardly studied.…”