Molecular characterization of Portuguese strains of <i>Yersinia ruckeri</i> isolated from fish culture systems

Sousa, Leonel; Magariños,; Eiras, J. C.; Toranzo,; Romalde, Jesús L.

doi:10.1046/j.1365-2761.2001.00281.x

Cited by 15 publications

(15 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The different LPS patterns exhibited by the Chilean isolates corresponded to those of serotypes O1a, O1b and O2b. Differences in the number of bands and the interband distances among serotypes are consistent with results previously reported for Y. ruckeri by other authors (Romalde et al 1993, Sousa et al 2001. Results of OMP analysis of the Chilean Y. ruckeri isolates supported those from previous studies (Romalde et al 1993, Sousa et al 2001, in which observations of distinct protein profiles were observed related to the different serotypes of Y. ruckeri.…”

Section: Discussionsupporting

confidence: 91%

“…Differences in the number of bands and the interband distances among serotypes are consistent with results previously reported for Y. ruckeri by other authors (Romalde et al 1993, Sousa et al 2001. Results of OMP analysis of the Chilean Y. ruckeri isolates supported those from previous studies (Romalde et al 1993, Sousa et al 2001, in which observations of distinct protein profiles were observed related to the different serotypes of Y. ruckeri.In addition, genetic variability was observed in Chilean Yersinia ruckeri isolates using REP-and ERIC-PCR techniques, establishing, respectively, 2 and 3 groups related with the serotype. ERIC-PCR showed higher discriminatory power than REP-PCR, but both techniques could have some value as tools to study ERM epidemiology.…”

supporting

confidence: 91%

“…Some studies reported the ability to ferment sorbitol as a distinguishing characteristic for strains of Y. ruckeri belonging to serotype O2 (Romalde et al 2003, Austin & Austin 2007. However, some isolates, including the different serotypes O1, O3 and O4, are also able to ferment sorbitol (Stevenson & Airdrie 1984, Sousa et al 2001. In this study, 1 Chilean isolate (6807) was able to ferment sorbitol and was confirmed by serological tests as belonging to serotype O2b.…”

Section: Discussionmentioning

confidence: 51%

See 2 more Smart Citations

Serological and molecular heterogeneity among Yersinia ruckeri strains isolated from farmed Atlantic salmon Salmo salar in Chile

Bastardo¹,

Bohle²,

Ravelo³

et al. 2011

Dis. Aquat. Org.

View full text Add to dashboard Cite

We investigated 11 strains of Yersinia ruckeri, the causative agent of enteric redmouth disease (ERM), that had been isolated from Atlantic salmon Salmo salar L. farmed in Chile and previously vaccinated against ERM. Phylogenetic analysis of the 16S rRNA gene sequences confirmed the identification of the salmon isolates as Y. ruckeri. A comparative analysis of the biochemical characteristics was made by means of traditional and commercial miniaturised methods. All studied isolates were motile and Tween 80 positive, and were identified as biotype 1. In addition, drug susceptibility tests determined high sensitivity to sulphamethoxazole/trimethroprim, oxytetracycline, ampicillin and enrofloxacin in all isolates. Serological assays showed the presence of O1a, O1b and O2b serotypes, with a predominance of the O1b serotype in 9 strains. Analysis of the lipopolysaccharide profiles and the correspondent immunoblot confirmed these results. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) of the outer membrane proteins revealed that all Chilean strains had profiles with a molecular weight range between 34 and 55 kDa, with 3 distinct groups based on differences in the major bands. Genotyping analyses by enterobacterial repetitive intergenic consensus (ERIC-) and repetitive extragenic palindromic (REP-)PCR techniques clearly indicated intraspecific genetic diversity among Chilean Y. ruckeri strains. KEY WORDS: Yersinia ruckeri · Enteric redmouth disease · ERM · Atlantic salmon · Serology · Outer membrane proteins · Lipopolysaccharide · GenotypingResale or republication not permitted without written consent of the publisher Dis Aquat Org 93: 207-214, 2011 can be grouped into clonal types on the basis of biotype, serotype and outer membrane protein (OMP) profiles (Davies 1991). Strains of serotypes O1a (classic serovar I) and O2b (classic serovar II) cause most epizootic outbreaks, and serotype O1a is predominant in cultured salmonids (Stevenson & Airdrie 1984, Romalde et al. 1993.ERM has been successfully controlled for decades by vaccination with monovalent killed whole cell commercial vaccines. Although formulations of most commercial vaccines are based only on serovar I (Hagerman strain), different degrees of cross-protection among serotypes have been reported (Stevenson & Airdrie 1984). In recent years, reports of ERM vaccine breakdown have emerged in Europe and the USA, which were mostly attributed to biotype 2 strains (Austin et al. 2003, Fouz et al. 2006, Arias et al. 2007, Wheeler et al. 2009).In Northern Europe and Chile, both of which are major production areas of farmed Atlantic salmon, the fry are routinely vaccinated with commercial rainbow trout ERM vaccines (Bravo & Midtlyng 2007, Wheeler et al. 2009 In this study we report the full phenotypic, serological and molecular characterisation of a group of Yersinia ruckeri strains that were isolated from these ERM epizootics in Atlantic salmon farmed in Chile. MATERIALS AND METHODSBacterial isolates. Several episodes of ERM with mo...

show abstract

Section: Discussionsupporting

confidence: 91%

supporting

confidence: 91%

Section: Discussionmentioning

confidence: 51%

See 1 more Smart Citation

Serological and molecular heterogeneity among Yersinia ruckeri strains isolated from farmed Atlantic salmon Salmo salar in Chile

Bastardo¹,

Bohle²,

Ravelo³

et al. 2011

Dis. Aquat. Org.

View full text Add to dashboard Cite

show abstract

“…This approach allowed six distinct clonal groups to be distinguished among serotype O1 isolates and was able to differentiate between the classical Hagerman and the newly emerged biotype 2 strains (21). Subsequently, OMP analysis has played an important role in various epidemiological analyses of Y. ruckeri (9,15,24,(29)(30)(31).…”

mentioning

confidence: 99%

Yersinia ruckeri Isolates Recovered from Diseased Atlantic Salmon (Salmo salar) in Scotland Are More Diverse than Those from Rainbow Trout (Oncorhynchus mykiss) and Represent Distinct Subpopulations

Ormsby

Caws

Burchmore

et al. 2016

Appl Environ Microbiol

View full text Add to dashboard Cite

Yersinia ruckeri is the etiological agent of enteric redmouth (ERM) disease of farmed salmonids. Enteric redmouth disease is traditionally associated with rainbow trout (Oncorhynchus mykiss, Walbaum), but its incidence in Atlantic salmon (Salmo salar) is increasing. Yersinia ruckeri isolates recovered from diseased Atlantic salmon have been poorly characterized, and very little is known about the relationship of the isolates associated with these two species. Phenotypic approaches were used to characterize 109 Y. ruckeri isolates recovered over a 14-year period from infected Atlantic salmon in Scotland; 26 isolates from infected rainbow trout were also characterized. Biotyping, serotyping, and comparison of outer membrane protein profiles identified 19 Y. ruckeri clones associated with Atlantic salmon but only five associated with rainbow trout; none of the Atlantic salmon clones occurred in rainbow trout and vice versa. These findings suggest that distinct subpopulations of Y. ruckeri are associated with each species. A new O serotype (designated O8) was identified in 56 biotype 1 Atlantic salmon isolates and was the most common serotype identified from 2006 to 2011 and in 2014, suggesting an increased prevalence during the time period sampled. Rainbow trout isolates were represented almost exclusively by the same biotype 2, serotype O1 clone that has been responsible for the majority of ERM outbreaks in this species within the United Kingdom since the 1980s. However, the identification of two biotype 2, serotype O8 isolates in rainbow trout suggests that vaccines containing serotypes O1 and O8 should be evaluated in both rainbow trout and Atlantic salmon for application in Scotland. IMPORTANCEVaccination plays an important role in protecting Atlantic salmon against the bacterial pathogen Yersinia ruckeri, but, in recent years, there has been an increasing incidence of vaccine breakdown in salmon. This is largely because current vaccines are aimed at rainbow trout and are based on serotypes specific for this species. A wider range of serotypes is responsible for infection in Atlantic salmon, but very little is known about the diversity of these strains and their relationships to those recovered from rainbow trout. In the present study, we demonstrate that Y. ruckeri isolates recovered from diseased Atlantic salmon in Scotland are more diverse than those from rainbow trout; furthermore, isolates from the two species represent distinct subpopulations. In addition, a new O serotype was identified that is responsible for a significant proportion of the disease in Atlantic salmon. Our findings are likely to have important implications for the development of improved vaccines against Y. ruckeri. The Gram-negative bacterium Yersinia ruckeri is the etiological agent of enteric redmouth (ERM) disease of cultured salmonids and causes significant economic losses to the fish-farming industry. Yersinia ruckeri was first isolated in 1956 from diseased rainbow trout (Oncorhynchus mykiss, Walbaum) in the Hagerman Valle...

show abstract

“…In the sampling carried out at day seven after the injection, Y. ruckeri was re-isolated from the visceral organs and blood of moribund fish using a tryptic soy agar (TSA) medium (Austin et al, 2003, Sousa et al, 2001. Hematological and non-specific immune parameters, oxidative stress indices (OSI) and histopathological examinations were analyzed in twenty-one fish from each experimental group (the total number of fish: 105).…”

Section: Experimental Infection With Yersinia Ruckerimentioning

confidence: 99%

Untitled

Şahan¹,

Duman²,

COLAK³

et al. 2017

Turk. J. Fish. Aquat. Sci.

View full text Add to dashboard Cite

The effect of the Yersinia ruckeri infection in the different tissues of 225 rainbow trout (Oncorhynchus mykiss) fed with rosehip (Rosa canina) was researched by evaluating a range of factors such as hematological and histopathological findings, non-specific immune parameters, liver antioxidant parameters, and then determined mortality and relative percentage survival (RPS) rates.The study comprises control groups (C+, C−) and rosehip experimental groups (REGs): R10 (10%), R20 (20%) and R30 (30%). Following the 50-day feeding period, the fish were infected with Y. ruckeri and then blood, liver, spleen and kidney samples were taken. RBC, WBC, Hb, Hct and RBC indices, non-specific immune parameters, NBT, phagocytic activity and leukocyte formulas all significantly increased in R20 compared to other groups. Antioxidant parameters SOD, CAT and GSH values increased in the R20 and R30 groups, and the lowest RPS value was detected in R30 at 26.31%, while it increased in the R20 group to 42.09%. R30 showed no significant hematological and immunological effects, however, the immune cells were suppressed. In this research, R20 was determined to be the ideal dose, taking into account both health indicators and hematological parameters, as a defense mechanism against Y. ruckeri, showing the best antibacterial and antioxidant effects.

show abstract

Molecular characterization of Portuguese strains of Yersinia ruckeri isolated from fish culture systems

Cited by 15 publications

References 27 publications

Serological and molecular heterogeneity among Yersinia ruckeri strains isolated from farmed Atlantic salmon Salmo salar in Chile

Serological and molecular heterogeneity among Yersinia ruckeri strains isolated from farmed Atlantic salmon Salmo salar in Chile

Yersinia ruckeri Isolates Recovered from Diseased Atlantic Salmon (Salmo salar) in Scotland Are More Diverse than Those from Rainbow Trout (Oncorhynchus mykiss) and Represent Distinct Subpopulations

Untitled

Contact Info

Product

Resources

About