Molecular Characterization of Porcine Rotaviruses from the Southern Region of Brazil: Characterization of an Atypical Genotype G[9] Strain

Rácz, Maria Lucia; Kroeff, Suzana S.; Munford, Veridiana; Caruzo, Thabata Alessandra Ramos; Durigon, Edison Luiz; Hayashi, Yasuyoshi; Gouvêa, Vera; Palombo, Enzo A.

doi:10.1128/jcm.38.6.2443-2446.2000

Cited by 43 publications

References 19 publications

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Evidence for zoonotic transmission of group C rotaviruses among children in Belém, Brazil

Gabbay

Borges

Oliveira

et al. 2008

Journal of Medical Virology

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The prevalence and potential zoonotic transmission of group C rotavirus (RVC) were examined by testing fecal samples collected from children during a longitudinal study that was carried out in the outskirts of Belém, Brazil, from December 1982 to March 1986. The study involved a group of 30 children who were followed from birth to 3 years. Of the 77 samples tested from 29 children, 5 (6.5%) were positive for human and 3 (4%) for porcine RVC by using nested PCR assay with primers specific for VP6 gene of human or porcine RVC and by Southern hybridization using a probe specific for VP6 gene of both human and porcine RVC. In addition, a total of 59 fecal specimens from the 30th child were tested, 1 (1.7%) and 14 (23.7%) were positive for human and porcine RVC, respectively. Partial nucleotide sequences of VP6 gene demonstrated that the six human strains detected in Brazil were homologous with other human RVC, and 14 of the 17 porcine RVC strains examined showed a complete homology among themselves but differed slightly from the porcine Cowden strain, suggesting that a single porcine RVC strain was circulating in Belém. This study is the first to provide evidence for transmission of RVC from swine to human. They also indicate that both human and porcine RVC were endemic in Belém.

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Evidence for zoonotic transmission of group C rotaviruses among children in Belém, Brazil

Gabbay

Borges

Oliveira

et al. 2008

Journal of Medical Virology

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Molecular Epidemiology of Human Rotaviruses in Latin America

Degiuseppe

Stupka

Parra

2017

Human Virology in Latin America

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Molecular characterization of a porcine Group A rotavirus strain with G12 genotype specificity

et al. 2006

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A porcine Group A rotavirus strain (RU172) was detected and molecularly characterized during a surveillance study conducted for rotavirus infection in a pig farm located in a suburban area of Kolkata City, India. The G12 genotype specificity of RU172 was revealed by PCR-based genotyping assays following addition of a G12 type-specific primer (designed in our laboratory to pick up G12 isolates from field samples) and was confirmed by sequence analysis of the VP7-encoding gene. The RU172 strain exhibited maximum VP7 identities of 93.6% to 94.5% with human G12 strains at the deduced amino acid level. In spite of its G12 genotype nature, RU172 appeared to be distinct from human G12 rotaviruses and, on phylogenetic analysis, formed a separate lineage with human G12 strains. Among the other gene segments analyzed, RU172 belonged to NSP4 genotype B, had a NSP5 and VP6 of porcine origin, and shared maximum VP4 identities with porcine P[7] rotaviruses (94.3%-95.4% at the deduced amino acid level). Therefore, to the best of our knowledge, this is the first report of detection of an animal rotavirus strain with G12 genotype specificity. Detection of strains like RU172 provides vital insights into the genomic diversity of Group A rotaviruses of man and animals.

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Molecular Characterization of Porcine Rotaviruses from the Southern Region of Brazil: Characterization of an Atypical Genotype G[9] Strain

Cited by 43 publications

References 19 publications

Evidence for zoonotic transmission of group C rotaviruses among children in Belém, Brazil

Evidence for zoonotic transmission of group C rotaviruses among children in Belém, Brazil

Molecular Epidemiology of Human Rotaviruses in Latin America

Molecular characterization of a porcine Group A rotavirus strain with G12 genotype specificity

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