Molecular characterization of outer membrane vesicles released from Acinetobacter radioresistens and their potential roles in pathogenesis

Fulsundar, Shweta; Kulkarni, Heramb M.; Jagannadham, Medicharla V.; Nair, Rashmi Ravindran; Keerthi, Sravani; Sant, Pooja; Pardesi, Karishma; Bellare, Jayesh; Chopade, Balu A.

doi:10.1016/j.micpath.2015.04.005

Cited by 29 publications

(20 citation statements)

References 71 publications

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“…Several studies on OMVs in Acinetobacter have suggested that they have many functions, including roles in horizontal gene transfer, antibiotic resistance, and virulence. A wide variety of cargo types have been identified in OMVs from different Acinetobacter strains, including virulence proteins, antibiotic resistance determinants, and DNA (96)(97)(98)(99)(100)(101)(102). An important virulence factor of A. baumannii, OmpA, has also been found to be associated with OMVs, and OMVs have been suggested to act as a mechanism for delivery for this protein to host cells (103).…”

Section: Protein Secretionmentioning

confidence: 99%

Pathogenic Acinetobacter: from the Cell Surface to Infinity and Beyond

2016

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The genus Acinetobacter encompasses multiple nosocomial opportunistic pathogens that are of increasing worldwide relevance because of their ability to survive exposure to various antimicrobial and sterilization agents. Among these, Acinetobacter baumannii, Acinetobacter nosocomialis, and Acinetobacter pittii are the most frequently isolated in hospitals around the world. Despite the growing incidence of multidrug-resistant Acinetobacter spp., little is known about the factors that contribute to pathogenesis. New strategies for treating and managing infections caused by multidrug-resistant Acinetobacter strains are urgently needed, and this requires a detailed understanding of the pathobiology of these organisms. In recent years, some virulence factors important for Acinetobacter colonization have started to emerge. In this review, we focus on several recently described virulence factors that act at the bacterial surface level, such as the capsule, O-linked protein glycosylation, and adhesins. Furthermore, we describe the current knowledge regarding the type II and type VI secretion systems present in these strains.

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Section: Protein Secretionmentioning

confidence: 99%

Pathogenic Acinetobacter: from the Cell Surface to Infinity and Beyond

2016

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“…[15][16][17][18][19][20][21] They have excellent biofilm-forming capability. [22][23][24][25] They have high degree of antibiotic and metal resistance. 16,26,27 Acinetobacter is known to synthesize gold, silver and platinum NPs.…”

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confidence: 99%

Green synthesis of selenium nanoparticles using<em> Acinetobacter</em> sp. SW30: optimization, characterization and its anticancer activity in breast cancer cells

Wadhwani

Gorain

Banerjee

et al. 2017

IJN

147

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The aim of this study was to synthesize selenium nanoparticles (SeNPs) using cell suspension and total cell protein of Acinetobacter sp. SW30 and optimize its synthesis by studying the influence of physiological and physicochemical parameters. Also, we aimed to compare its anticancer activity with that of chemically synthesized SeNPs in breast cancer cells. Cell suspension of Acinetobacter sp. SW30 was exposed to various physiological and physicochemical conditions in the presence of sodium selenite to study their effects on the synthesis and morphology of SeNPs. Breast cancer cells (4T1, MCF-7) and noncancer cells (NIH/3T3, HEK293) were exposed to different concentrations of SeNPs. The 18 h grown culture with 2.7×10 9 cfu/mL could synthesize amorphous nanospheres of size 78 nm at 1.5 mM and crystalline nanorods at above 2.0 mM Na 2 SeO 3 concentration. Polygonal-shaped SeNPs of average size 79 nm were obtained in the supernatant of 4 mg/mL of total cell protein of Acinetobacter sp. SW30. Chemical SeNPs showed more anticancer activity than SeNPs synthesized by Acinetobacter sp. SW30 (BSeNPs), but they were found to be toxic to noncancer cells also. However, BSeNPs were selective against breast cancer cells than chemical ones. Results suggest that BSeNPs are a good choice of selection as anticancer agents.

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“…To determine the size of each bacterium and the produced OMVs, a number of samples were analysed using SEM ( n = 21 ± 1) and DLS ( n = 2,500 ± 50). Both techniques have been employed in similar studies (Fulsundar et al, 2015; Hong et al, 2009; Ionescu et al, 2014; Oliver et al, 2016). In the case of measuring bacteria by SEM, a single bacterium was defined as being separated or having well‐defined borders, excluding bacteria that were agglomerated or in a state of division.…”

Section: Resultsmentioning

confidence: 97%

Identification and characterization of outer membrane vesicles from the fish pathogen Vibrio ordalii

Echeverría-Bugueño

Espinosa-Lemunao

Irgang

et al. 2020

Journal of Fish Diseases

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Vibriosis outbreaks due to Vibrio ordalii occur globally, but Chilean salmon aquaculture, in particular, has suffered significant monetary losses in the last 15 years. Little is known about the virulence mechanisms employed by V. ordalii. However, most Vibrio pathogens (e.g., Vibrio anguillarum, a very close taxonomic species) present outer membrane vesicles (OMVs) that are released extracellularly and implicated in the delivery of virulence factors to host cells. This study provides the first reported evidence of the fish pathogen V. ordalii producing and releasing OMVs under normal growth conditions. Analyses were conducted with the V. ordalii strain Vo‐LM‐18 and the type strain ATCC 33509T. For comparative purposes, the reference strain V. anguillarum ATCC 43307 was employed. The average size for the three Vibrio strains was 0.215 ± 0.6 µm (via scanning electron microscopy) or between 0.19 and 1.8 µm (via dynamic light scattering), with each bacterium presenting a wide range. SDS‐PAGE revealed similarities in OMV patterns, but neither total nor external proteins were identical. Comparing V. ordalii ATCC 33509T and Vo‐LM‐18, bands were most evident in the total proteins, and the greatest degree of similarity in OMV profiles was between 37 and 50 kDa. The purified OMVs demonstrated haemolytic enzyme activity, which could play a role during V. ordalii infection. These data represent an initial step towards gaining new insights into this virulence factor, of which a lot is known in other pathogenic microorganisms.

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Molecular characterization of outer membrane vesicles released from Acinetobacter radioresistens and their potential roles in pathogenesis

Cited by 29 publications

References 71 publications

Pathogenic Acinetobacter: from the Cell Surface to Infinity and Beyond

Pathogenic Acinetobacter: from the Cell Surface to Infinity and Beyond

Green synthesis of selenium nanoparticles using<em> Acinetobacter</em> sp. SW30: optimization, characterization and its anticancer activity in breast cancer cells

Identification and characterization of outer membrane vesicles from the fish pathogen Vibrio ordalii

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