Molecular characterization of Mungbean yellow mosaic India virus infecting tomato (Solanum lycopersicum L.)

Agnihotri, Aniruddha Kumar; Mishra, Sudhakar Prasad; Ansar, Mohammad; Tripathi, Ramesh C.; Singh, Ravindra; Akram, Mohammad

doi:10.1007/s13313-018-0611-7

Cited by 13 publications

(4 citation statements)

References 32 publications

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“…In all three cases the symptoms of the infections suggested that the betasatellite was enhancing symptoms –the symptoms in each case having features not usually associated with MYMIV infection. MYMIV has on one occasion been identified in tomato without, apparently, the presence of a betasatellite 47 . The MYMIV identified in tomato in that case was also of the pseudo-recombinant type, with a DNA B originating from MYMV.…”

Section: Discussionmentioning

confidence: 99%

Frequent occurrence of Mungbean yellow mosaic India virus in tomato leaf curl disease affected tomato in Oman

Shahid

Shafiq

Ilyas

et al. 2019

Sci Rep

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Next generation sequencing (NGS) of DNAs amplified by rolling circle amplification from 6 tomato (Solanum lycopersicum) plants with leaf curl symptoms identified a number of monopartite begomoviruses, including Tomato yellow leaf curl virus (TYLCV), and a betasatellite (Tomato leaf curl betasatellite [ToLCB]). Both TYLCV and ToLCB have previously been identified infecting tomato in Oman. Surprisingly the NGS results also suggested the presence of the bipartite, legume-adapted begomovirus Mungbean yellow mosaic Indian virus (MYMIV). The presence of MYMIV was confirmed by cloning and Sanger sequencing from four of the six plants. A wider analysis by PCR showed MYMIV infection of tomato in Oman to be widespread. Inoculation of plants with full-length clones showed the host range of MYMIV not to extend to Nicotiana benthamiana or tomato. Inoculation to N. benthamiana showed TYLCV to be capable of maintaining MYMIV in both the presence and absence of the betasatellite. In tomato MYMIV was only maintained by TYLCV in the presence of the betasatellite and then only at low titre and efficiency. This is the first identification of TYLCV with ToLCB and the legume adapted bipartite begomovirus MYMIV co-infecting tomato. This finding has far reaching implications. TYLCV has spread around the World from its origins in the Mediterranean/Middle East, in some instances, in live tomato planting material. The results here may suggest that begomoviruses which do not commonly infect tomato, such as MYMIV, could be spread as a passenger of TYLCV in tomato.

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Section: Discussionmentioning

confidence: 99%

Frequent occurrence of Mungbean yellow mosaic India virus in tomato leaf curl disease affected tomato in Oman

Shahid

Shafiq

Ilyas

et al. 2019

Sci Rep

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“…Begomoviruses infecting legumes are unique and genetically different from other begomoviruses infecting the rest of agricultural and horticultural crops (Agnihotri et al, 2019). These DNA viruses are endemic in distribution and present only in Indian subcontinent and Southeast Asia and proposed as a distinct group ‘‘Legumovirus’’ known to infect grain legumes and weeds.…”

Section: Discussionmentioning

confidence: 99%

“…In southern Asia, grain legume crops are suffering huge loss due to attack of different begomoviruses (Ilyas et al, 2009; Qazi et al, 2007). Begomoviruses infecting legumes are grouped under legumoviruses, which mainly consist of horsegram yellow mosaic virus (HgYMV), dolichos yellow mosaic virus (DoYMV), mungbean yellow mosaic virus (MYMV), mungbean yellow mosaic India virus (MYMIV), which are known to cause yellow mosaic disease (YMD) of leguminous crop in India (Agnihotri et al, 2019). Apart from these, other viruses which are reported on legume crops are tobacco curly shoot virus (TbCSV) (Venkataravanappa et al, 2012), french bean leaf curl virus (FbLCV) (Kamaal et al, 2013), tomato leaf curl Gujarat virus (ToLCGV) (Kamaal et al, 2015) and tomato leaf curl Joydebpur virus (ToLCJV) (Ansar et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and evidence of recombination ofHorsegram yellow mosaic virusinfecting pole bean (Phaseolus vulgarisL.) from South India

Bindu

Hiremath

et al. 2023

Preprint

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The yellow mosaic disease (YMD) caused by begomoviruses is a major constraint for the production of pole bean (Phaseolus vulgaris L.) in India. Survey was carried out in the eastern dry zone of Karnataka during 2019-20 to record the incidence of yellow mosaic disease in pole bean which revealed the ubiquitous prevalence of YMD in pole bean ranging from 6.02 to 80.74 per cent. Leaf samples collected (symptomatic and asymptomatic) were subjected for begomovirus detection using specific primers. Twelve samples, representing all the 12 taluks in the surveyed region were considered for full genome amplification by RCA, cloned and sequenced. Genome length of 12 current isolates ranged from 2718 - 2744 and 2668 - 2671 nucleotides for DNA-A and DNA-B, respectively. Sequence analysis using Sequence Demarcation Tool (SDT) showed >91 per cent nucleotide identity of current isolates (DNA-A) with other horsegram yellow mosaic virus (HgYMV) isolates available in the GenBank. As per existing ICTV criteria, all the current isolates can be considered as strains of HgYMV. Further, DNA-B associated with all the 12 isolates also shared >91 per cent nucleotide identity with DNA-B of HgYMV isolates, indicating absence of component re-assortment in HgYMV. Variation in the pairwise nucleotide identity and phylogenetic analysis confirmed the existence of new strains within the current HgYMV isolates. GC plot analysis reveals potential recombination in the low GC-rich regions. Further, recombination breakpoint analysis indicated intra-species recombination in both DNA-A and DNA-B, which might have driven the origin of new strains in HgYMV. This is the first comprehensive study on begomoviruses isolates associated with the yellow mosaic disease of pole bean based on complete genome sequencing in the world.

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“…Weeds are also alternative hosts, where economically important pathogens can survive between crop cycles (Mubin et al, 2010;Papayiannis et al, 2011;Wyant et al, 2011;Jyothsna et al, 2013). For example, whitefly-transmitted geminiviruses (Begomovirus; Geminiviridae) are known to infect economically important crops as well as weed hosts (Seal et al, 2006;Mubin et al, 2009;Ansar et al, 2019;Agnihotri et al, 2019). Tomato leaf curl New Delhi virus (ToLCNDV) is an important bipartite geminivirus in Begomovirus, which infects approx.…”

Section: Introductionmentioning

confidence: 99%

Nightshade (Solanum nigrum), an intermediate host between tomato and cucurbits of Tomato leaf curl New Delhi virus

Ansar

Agnihotri

Ranjan

et al. 2021

Phytopathol. Mediterr.

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Geminiviruses infect many crop plants, and are limiting factors for vegetable crop production. Begomoviruses (Geminiviridae) cause typical symptoms of leaf curling and puckering in nightshade (Solanum nigrum), a seasonal weed in Bihar, India. To investigate if nightshade was an intermediate host for begomovirus, virus DNA was extracted and characterized. The DNA-A of the virus yielded 2737 nt and DNA-B yielded 2706 nt. The intergenic region (IR) showed a conserved nonanucleotide sequence that potentially forms a stem-loop structure. The genomic sequence of DNA-A shared 94% identity with that of Tomato leaf curl New Delhi virus (ToLCNDV)-ivy gourd isolate. However, the sequence of DNA-B showed 95% identity with a bitter gourd isolate. PCR-based detection revealed the presence ToLCNDV in bottle gourd, pumpkin, sponge gourd, and bitter gourd. The IR sequences of the viruses isolated from these cucurbits and tomato were 100% identical. Whitefly-mediated transmission of the virus to cucurbits and tomato from nightshade was also demonstrated. These results indicate that nightshade may act as reservoir of ToLCNDV, and is involved in developing epidemics in cucurbit species. The strain of ToLCNDV has probably adapted from solanaceous to cucurbitaceous hosts. This is the ﬁrst report of ToLCNDV infecting nightshade in India, highlighting this virus as a possible cause of disease epidemics in economically important cucurbits.

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Molecular characterization of Mungbean yellow mosaic India virus infecting tomato (Solanum lycopersicum L.)

Cited by 13 publications

References 32 publications

Frequent occurrence of Mungbean yellow mosaic India virus in tomato leaf curl disease affected tomato in Oman

Frequent occurrence of Mungbean yellow mosaic India virus in tomato leaf curl disease affected tomato in Oman

Genetic diversity and evidence of recombination ofHorsegram yellow mosaic virusinfecting pole bean (Phaseolus vulgarisL.) from South India

Nightshade (Solanum nigrum), an intermediate host between tomato and cucurbits of Tomato leaf curl New Delhi virus

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