Molecular Characterization of Fowl Adenovirus D Species in Broiler Chickens with Inclusion Body Hepatitis in Egypt

Elbestawy, Ahmed R.; Ibrahim, Mahmoud; Hammam, H. M.; Noreldin, Ahmed E.; Bahrawy, Amanallah El; Ellakany, Hany F.

doi:10.5455/ajvs.74411

Cited by 10 publications

(15 citation statements)

References 33 publications

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“…IBDV and CIAV have been reported as common co-infection of FAdVs positive flocks which exacerbate the death rates as described by (Elbestawy et al, 2020). Despite this, there have been reports that IBH can cause mortality even when no other infective agent is present (Mohamed et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…The obtained sequences were clustered with fowl Adenoviruses strains isolated from Europe, Brazil, Malaysia, China, Japan, and India. Many countries all over the world reported detection of FAdV-D including Canada (Ojkic et al, 2008), South Africa (Maartens et al, 2014), China (Changjing et al, 2016), Poland (Niczyporuk, 2016), Lebanon (Shaib et al, 2017), Saudi Arabia (Mohamed et al, 2018) and Egypt (El-Tholoth and Abou El-Azm, 2019; Elbestawy et al, 2020). In addition, Radwan et al, 2019 previously detected two strains of serotype FAdV-8a (FAdV-E) in Egyptian chicken farms during 2015.…”

Section: Discussionmentioning

confidence: 99%

“…Detection of accompanied infections with infectious bursal disease virus (IBDV) and chicken infectious anemia virus (CIAV) was performed using the extracted viral DNA/RNA from the liver for CIAV and bursa of Fabricius tissues for IBDV from Adenovirus positive cases by conventional PCR assay as previously reported by Metwally et al (2009) and Elbestawy et al (2020).…”

Section: Detection Of Concurrent Infection With Infectious Bursal Disease Virus and Chicken Infectious Anemia Virus By Conventional Pcrmentioning

confidence: 99%

“…Hexon is the main adenovirus protein and is typically used to detect FAdVs molecularly. Further FAdV typing was carried out using restriction enzyme length polymorphism (RFLP) and/or DNA sequencing (Hess, 2000) In Egypt, FAdVs outbreaks have been reported and confirmed by the sequence analysis of the viruses in collected samples (El-Tholoth and Abou El-Azm, 2019;Radwan et al, 2019;Elbestawy et al, 2020). Therefore, the current study was conducted for isolation, molecular characterization, typing, and histopathological picture of the most recent FAdVs circulating in broiler farms suffering from IBH/HPS in different Egyptian governorates.…”

Section: Introductionmentioning

confidence: 99%

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Isolation and Molecular Characterization of Fowl Adenoviruses Associated with Inclusion Body Hepatitis-hydropericardium Syndrome in Broiler Chickens in Egypt

Naguib¹,

Morsy²,

Samir³

et al. 2021

WVJ

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Avian adenoviruses are an extremely diversified group of pathogens that recently triggering a variety of problems for poultry production. In particular, Inclusion Body Hepatitis-Hydropericardium Syndrome (IBH-HPS), which has been observed in broiler birds from 3 to 6 weeks of age and is associated with anemia, hemorrhagic disorders, hydropericardium, and high mortality. The disease has been reported worldwide, and recently it was reported in many Egyptian farms, causing severe economic losses. Therefore, the current study aimed to isolate, and genetically type the most common Adenovirus serotypes associated with this syndrome in Egyptian farms. A total of 50 broiler chicken farms (3-6 weeks old) located in different Egyptian governorates were examined. Macroscopically, the diseased flock revealed hydropericardium, enlarged friable livers with ecchymotic hemorrhages, and varying mortality rates (1 to 7.5%). Histopathologically, severe diffuse necrotizing enteritis, hepatitis, pericarditis, and diffuse lymphoid depletion of the spleen were the most prominent lesions. Liver tissues and cloacal swabs were collected from all examined flocks for FAdVs detection by conventional polymerase chain reaction (PCR) targeting the L1 loop in the hexon gene. The PCR products were sequenced for typing of the detected viruses. It was found that 10 out of 50 flocks examined were PCR positive for FAdVs (20%). Phylogenetic analysis of the sequenced genes revealed that the obtained viruses clustered with reference strains belonging to FAdV type D and E serotype 2, 11, and 8a respectively. The isolation of both FAdV type D and FAdV type E were carried out on a primary cell culture chicken embryo liver cell (CEL) and the presence of these viruses was confirmed by PCR after the appearance of cytopathic effect (CPE). From this study, it could be concluded that both FAdVs types D and E are the most common adenoviruses circulating in poultry farms suffering from hydropericardium and inclusion body hepatitis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Detection Of Concurrent Infection With Infectious Bursal Disease Virus and Chicken Infectious Anemia Virus By Conventional Pcrmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Isolation and Molecular Characterization of Fowl Adenoviruses Associated with Inclusion Body Hepatitis-hydropericardium Syndrome in Broiler Chickens in Egypt

Naguib¹,

Morsy²,

Samir³

et al. 2021

WVJ

View full text Add to dashboard Cite

show abstract

“…In Egypt, FAdVs in the poultry population have drawn little interest, which has resulted in no vaccination measures have been developed against such viruses in the various poultry sectors. While there are limited studies about the current situation of FAdVs, there have been reports on the spread of the serotypes which induce IBH, including FAdV-8a ( Radwan et al., 2019 ) and FAdVs 2/11, which belong to the FAdVs species D ( El-Tholoth and Abou El-Azm, 2019 ; Elbestawy et al., 2020 ). The need to increase awareness about the consequences of FAdV infections in Egypt makes it essential to investigate their impact and evolution.…”

Section: Introductionmentioning

confidence: 99%

Epidemiological and molecular analysis of circulating fowl adenoviruses and emerging of serotypes 1, 3, and 8b in Egypt

Adel

Halim

Samir

et al. 2021

Heliyon

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Fowl adenoviruses (FAdVs) are a large group of viruses of different serotypes. They are responsible for inclusion body hepatitis, adenoviral gizzard erosion, and hepatitis hydropericardium syndrome. The present study presents a comprehensive overview of FAdVs in Egypt, with a focus on the epidemiological features of virus serotypes across the country. We conducted molecular investigation of multiple FAdV species based on the genetic signature of hypervariable regions 1–4 in the loop1 (L1) region of the hexon gene. Epidemiologically, the Nile Delta governorates showed high positivity of FAdVs, which were more commonly found in broilers than in layers. Genetically, species D and serotype 8a/E dominated, and the findings also revealed the emergence of new FAdV serotypes 1, 3, and 8b. The comparative analysis of hypervariable regions in the L1 region of the hexon gene revealed variables specific to each virus serotype. In silico predictions of L1 region revealed variations in the molecular structure and predicted the antigenic epitopes which may affect the cross-antigenicity between the different FAdV species and serotypes.

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Molecular characterization of aviadenovirus serotypes and pathogenicity of the identified adenovirus in broiler chickens

Lebdah¹,

Alshaya²,

Jalal³

et al. 2022

Poultry Science

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Molecular Characterization of Fowl Adenovirus D Species in Broiler Chickens with Inclusion Body Hepatitis in Egypt

Cited by 10 publications

References 33 publications

Isolation and Molecular Characterization of Fowl Adenoviruses Associated with Inclusion Body Hepatitis-hydropericardium Syndrome in Broiler Chickens in Egypt

Isolation and Molecular Characterization of Fowl Adenoviruses Associated with Inclusion Body Hepatitis-hydropericardium Syndrome in Broiler Chickens in Egypt

Epidemiological and molecular analysis of circulating fowl adenoviruses and emerging of serotypes 1, 3, and 8b in Egypt

Molecular characterization of aviadenovirus serotypes and pathogenicity of the identified adenovirus in broiler chickens

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