Molecular characterization of field isolates and vaccine strains of infectious bursal disease virus

Juneja, Surbhi; Ramneek,; Deka, Dipak; Oberoi, M. S.; Singh, Amarjit

doi:10.1016/j.cimid.2007.03.001

Cited by 9 publications

(6 citation statements)

References 26 publications

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“…The serological results detected viral antigen in the bursa at the early stages of the infection. The RT-PCR/RFLP pattern was used for differential pathotyping of the isolates (15,18 In immunosupression test GMT HI titers of the vaccinated and the control groups were calculated 5.07 vs 5.14 and no significant difference was found between them. The B:B index was estimated 1.14 following vaccination with the developed IBD live vaccine.…”

Section: Discussionmentioning

confidence: 99%

Isolation, Characterization and Standardization of New Infectious Bursal Disease Virus for Development of a Live Vaccine

et al. 2013

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Background and Aims: Infectious bursal disease (IBD) is an acute contagious viral disease of birds worldwide. The causative virus induces a persistent immune suppression following destroy B lymphocytes precursors in bursal lymphoid follicles. Vaccination is the main strategy for prevention of the disease in commercial poultry industry. Materials and Methods:To produce a live vaccine against the disease, an new virus strain was isolated from the affected bursa of Fabricius. Diagnostic serological tests, histopathology and molecular examinations were done for pathotyping the isolate. Results:The results indicated that the virus is a new strain and named IBD07IR. In case of developing live IBD vaccine, the bacteriology, purity, titration, reversion to virulence, bursabody index, immunosupression and efficacy tests were performed on the candidate vaccine virus seed. The live vaccine was produced following propagation of the IBDV seed in SPF embryonated eggs. The proper dose of the vaccine was found by administration of SPF chicken groups. Afterward, the laboratory trial was scaled and the clinical trials were done three times with different administration routs. Conclusion: According to serological assays and challenge, the developed live IBD vaccine induces an adequate immunity in both SPF and commercial chickens and had no adverse effects.

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Section: Discussionmentioning

confidence: 99%

Isolation, Characterization and Standardization of New Infectious Bursal Disease Virus for Development of a Live Vaccine

et al. 2013

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“…Our results are also in correspondence with Shoshtari et al (2004), who reported the presence of single RE site for StuI in vvIBDV. Juneja et al (2008) also reported that StuI restriction site was present only in vvIBDV and not found in vaccine strains.…”

Section: Figmentioning

confidence: 99%

“…De Paula et al (2004) reported that an amino acid substitution was observed in the SspI site in non-vvIBDV strains. Juneja et al (2008) reported the presence of SspI restriction enzyme site in all the fi eld isolates and not in vaccine strain (Lin et al, 1993;Hoque et al, 2001). Th e vvIBDV isolates, 88180 and HK46 did not have site for SspI (Eterradossi et al, 1999;Lim et al, 1999).…”

Section: Figmentioning

confidence: 99%

Genotypic characterization of Indian isolates of infectious bursal disease virus strains by reverse transcription-polymerase chain reaction combined with restriction fragment length polymorphism analysis

Priyadharsini¹,

Senthilkumar²,

Raja³

et al. 2016

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Summary. -Th e reverse transcription PCR (RT-PCR) combined with restriction fragment length polymorphism (RFLP) is used for the diff erentiation of classical virulent (cv), virulent (v) and very virulent (vv) strains of infectious bursal disease virus (IBDV) isolates from chicken bursal tissues in southern states of India. In the present study, six diff erent isolates (MB11, HY12, PY12, BGE14, VCN14 and NKL14) of IBDV strains were subjected for genotyping along with vaccine virus (Georgia, intermediate strain) using RT-PCR for amplifi cation of a 743 bp sequence in the hypervariable region of VP2 gene followed by restriction enzyme digestion with 5 diff erent restriction enzymes (BspMI, SacI, HhaI, StuI and SspI). Th e RT-PCR products obtained from vvIBDV strains were digested by SspI enzyme except PY12, BGE14 and MB11 isolates. Th e SacI digested the isolate MB11, PY12 and the vaccine strain, but it did not cleave the very virulent isolates of IBDV. HhaI cleaved all the isolates with diff erent restriction profi le patterns. StuI digested all the vvIBDV isolates and BspMI was not able to diff erentiate fi eld isolates from vaccine strain. Th ough RT-PCR combined with RFLP is a genotypic method, further confi rmation of serotypes to distinguish the vvIBDV from cvIBDV has to be carried out using pathogenicity studies.Keywords: infectious bursal disease virus; reverse transcription polymerase chain reaction; restriction fragment length polymorphism; VP2 gene; genotyping * Corresponding author. E-mail: tmaskumar@yahoo.com, tmaskumar@tanuvas.org.in; phone: +91-44-25369301. Abbreviations: RT-PCR = reverse transcription PCR; RFLP = restriction fragment length polymorphism; cvIBDV = classical virulent infectious bursal disease virus; vvIBDV = very virulent infectious bursal disease virus

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“…The infectious bursal disease virus has two serotypes; however, only serotype I viruses are naturally pathogenic to chickens [12]- [13]. Serotype I strains are classified as very virulent IBDV strains, classic or variant and differ in their virulent, antigenic, and pathogenic properties [14]- [15]. A second serotype (serotype II), isolated from a fowl, turkeys, and ducks.…”

Section: Introductionmentioning

confidence: 99%

Detection and Identification of Infectious Bursal Disease Virus in Broiler Farms in Sulaimani Province

2016

IJACEBS

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The bursal Infectious disease (IBD) is an acute and highly contagious viral infection of immature chickens. A total of 76 poultry farms in areas around Sulaimania region was sampled to investigate the infection with the infectious bursal disease in broiler chickens. Out of 76 poultry farms, 28 (36.8 %) were infected with IBD during the period from October 2012 to March 2013. The significant clinical symptoms were a sudden drop in feed and water consumption, severe depression, watery white droppings and high mortality, at necropsy, there are hemorrhages in leg muscles, degeneration of the pectoral muscle, white mass (urate deposit) in the kidneys and cloaca are frequently observe during a post-mortem examination. Also, hemorrhagic & swollen bursa filled with strawcolored fluid was identified in few cases. Histopathology revealed hyperplasia of the reticuloendothelial cells and expansion of interfollicular connective tissue with edema of the affected bursa of Fabricius. The nucleotide sequence of Sulaimani IBDV isolates showed %99 homology and they also showed %96-%99 similarity with other IBDV strains. Regarding phylogenetic tree, construction based on the VP2 hypervariable region of IBDV nucleotide sequence alignment of the 16 genomes. IBD virus was divided into two general groups, namely Very virulent 1 (VVI) and Very virulent two (VVII). The topology of the tree indicated that the field isolates Sulaimania 01, 2012 and Sulaimania 02 2012 belong to very virulent IBDV strains that contain Indian and Egyptian Isolate. An outbreak of infectious bursal disease affecting 20-45 days old broiler chickens was investigated for the first time in Sulaimani region. This research is the first occurrence of infectious bursal disease in Sulaimania region/Iraq by RT-PCR.

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Molecular characterization of field isolates and vaccine strains of infectious bursal disease virus

Cited by 9 publications

References 26 publications

Isolation, Characterization and Standardization of New Infectious Bursal Disease Virus for Development of a Live Vaccine

Isolation, Characterization and Standardization of New Infectious Bursal Disease Virus for Development of a Live Vaccine

Genotypic characterization of Indian isolates of infectious bursal disease virus strains by reverse transcription-polymerase chain reaction combined with restriction fragment length polymorphism analysis

Detection and Identification of Infectious Bursal Disease Virus in Broiler Farms in Sulaimani Province

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