Molecular characterization and interviral relationships of a flexuous filamentous virus causing mosaic disease of sugarcane (Saccharum officinarum L.) in India

Abstract

A virus isolate causing mosaic disease of commercial sugarcane was purified to homogeneity. Electron microscopy revealed flexuous filamentous virus particles of ca 890 x 15 nm. The virus isolate reacted positively with heterologous antiserum to narcissus latent virus form UK, but failed to react with potyvirus group specific antiserum. N-terminal sequencing of the intact coat protein (CP) and the tryptic peptides indicated that the virus was probably a potyvirus but distinct from several reported potyviruses. Comparison of the 3'-terminal 1084 nucleotide sequence of the RNA genome of this virus revealed 93.6% sequence identity in the coat protein coding region with the recently described sugarcane streak mosaic virus (Pakistani isolate). The molecular weight of the coat protein (40 kDa) was higher than that deduced from the amino acid sequence (34 kDa). The apparent increase in size was shown to be due to glycosylation of the coat protein which has not been reported thus far in the family, Potyviridae. This is the first report on the molecular characterization of a virus causing mosaic disease of sugarcane in India and the results demonstrate that the virus is a strain of sugarcane streak mosaic virus, a member of the Tritimovirus genus of the Potyviridae. We have named it sugarcane streak mosaic virus--Andhra Pradesh isolate (SCSMV-AP).

First occurrence of Sugarcane streak mosaic virus infecting sugarcane in Indonesia

“…1a), and PS 864 variety was predominantly affected with from 1 to 62% of the plants having symptoms. SCSMV, a virus that causes streak mosaic of sugarcane, was first reported by Hall et al (1998) and has been found in Pakistan, India, Bangladesh, Sri-Lanka, Vietnam and Thailand (Chatenet et al 2005;Hema et al 1999). Based on phylogenetic analyses, Rabenstein et al (2002) and Hema et al (2002) considered that SCSMV is not in the genus Tritimovirus, but should be placed in a new genus in Potyviridae.…”

Genetic variability and potential recombination events in the HC-Pro gene of sugarcane streak mosaic virus

“…In India, SCSMV was first reported during 1999 [9], and since then, it is reported to have spread into different geographical locations, limiting sugarcane production [10,11]. Earlier reports on the genetic diversity of SCSMV based on coat protein (CP) gene sequences revealed the existence of high levels of genetic variation among Indian SCSMV isolates [11], and the isolates were differentiated into three clusters.…”

Detección del Virus del mosaico de la caña de azúcar (SCMV) en Saccharum spp. en México y origen filogenético de un aislado de Jalisco

“…Recently, Balarabe et al (2014) mentioned that the Johnsongrass mosaic virus (JGMV) and the Maize dwarf mosaic virus (MDMV) cause mosaic disease in sugarcane in Nigeria. To this day, the presence of the SrMV has been confirmed in the United States, China, and Vietnam (Yang and Mirkov, 1997;Grisham and Pan, 2007;Ha et al, 2008;Zhang et al, 2016), whereas SCSMV is limited to the sugarcane producing areas of the Asian continent (Hall et al, 1998;Hema et al, 1999;Rao et al, 2006). However, due to the proximity of Mexico to the United States, it is likely that SrMV is infecting sugarcane plants in our country.…”

“…(Grisham, 1994;Xie et al, 2009), o incluso aquellas muestras con mosaicos severos y positivas al SCMV contengan infecciones virales mixtas. Recientemente Balarabe et al (2014) , 1997;Grisham y Pan, 2007;Ha et al, 2008;Zhang et al, 2016), mientras que el SCSMV está limitado para las zonas cañeras del continente asiático (Hall et al, 1998;Hema et al, 1999;Rao et al, 2006). Sin embargo, debido a la cercanía de Estados Unidos con México sería muy probable que el SrMV se encuentre infectando a las plantas de caña de azúcar en nuestro país.…”