Molecular characterization and genetic relationship among almond cultivars assessed by RAPD and SSR markers

Shiran, Behrouz; Amirbakhtiar, Nazanin; Kiani, S H; Mohammadi, Shima; Sayed-Tabatabaei, Badraldin Ebrahim; Moradi, H.

doi:10.1016/j.scienta.2006.10.024

Cited by 70 publications

(55 citation statements)

References 44 publications

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“…Penanda ISSR (Chezhian et al, 2010), kombinasi antara RAPD dan ISSR (Josiah et al, 2008), RAPD dan mikrosatelit (Shiran et al, 2007), serta mikrosatelit (Butcher et al, 2004;Zelener et al, 2005;Jones et al, 2006;Nurtjajaningsih et al, 2007;Millar et al, 2008) telah digunakan untuk mendeteksi keragaman genetik di kebun benih. Penanda mikrosatelit umumnya digunakan karena penanda ini sangat polimorfik, bersifat codominan, netral dan dapat diamplifikasi dengan sejumlah kecil DNA (Millar et al, 2008).…”

Section: Pendahuluanunclassified

KERAGAMAN GENETIK TETUA DAN ANAKAN DARI KEBUN BENIH SEMAI Acacia mangium GRUP D (AM004) DI SUMATERA SELATAN, INDONESIA

Yuskianti¹

2014

JPKW

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ABSTRAKInformasi keragaman genetik dari satu generasi ke generasi selanjutnya merupakan faktor penting untuk pengelolaan dan konservasi genetik kebun benih. Tujuan dari penelitian ini adalah untuk mengevaluasi tingkat keragaman genetik tetua sebagai generasi pertama (F1) dan anakannya (F2) dari kebun benih Akasia mangium yang ada di kebun benih semai Grup D (AM004) di Sumatera Selatan. Analisis dilakukan terhadap 251 tetua dan ± 200 biji dari 10 pohon induk. Analisis menggunakan 12 penanda mikrosatelit menunjukkan bahwa rata-rata jumlah alel yang terdeteksi (A) dari semua tetua dan anakannya adalah 8,23 dan 7.08. Secara umum, tingkat keragaman genetik tetua dan anakan tidak berbeda (He=0.609 untuk tetua dan He=0.606 untuk anakan). Penelitian ini mendeteksi keberadaan alel baru pada anakan. Kondisi ini menunjukkan kemungkinan adanya kontaminasi serbuk sari dari luar kebun benih.Kata kunci: Keragaman genetik, kebun benih semai Sumatera Selatan, tetua, anakan, penanda mikrosatelit ABSTRACT Information on genetic diversity from one generation to its next generation is an important factor for management and conservation in a seed orchard. The purpose of this study was to evaluate the level of genetic diversity of parental as the first generation (F1) and offspring (F2) of Acacia mangium in the

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Section: Pendahuluanunclassified

KERAGAMAN GENETIK TETUA DAN ANAKAN DARI KEBUN BENIH SEMAI Acacia mangium GRUP D (AM004) DI SUMATERA SELATAN, INDONESIA

Yuskianti¹

2014

JPKW

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“…These markers have been used for the molecular characterization and estimation of genetic diversity among different Prunus species, including peach Bouhadida et al, 2007Bouhadida et al, , 2011Dirlewanger et al, 2002 andMartínez-Gómez et al, 2003a), sweet cherry (Wunsch and Hormaza, 2002) and apricot cultivars (Hormaza, 2002;Maghuly et al, 2005). Moreover, SSRs are currently being employed for molecular characterization, estimation of genetic diversity and genetic relationships among almond cultivars (Gouta et al, 2010) and other related species (Martínez-Gómez et al, 2003b;Sánchez-Pérez et al, 2006;Shiran et al, 2007;Xu et al, 2004;Zeinalabedini et al, 2008), but to our knowledge, no population identification of the Tunisian almond germplasm was carried out and molecular variance (AMOVA) analysis is still lacking.…”

Section: Introductionmentioning

confidence: 99%

Molecular variance of the Tunisian almond germplasm assessed by simple sequence repeat (SSR) markers

Gouta¹,

Ksia²,

Ahmed³

et al. 2013

Afr. J. Biotechnol.

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The genetic variance analysis of 82 almond (Prunus dulcis Mill.) genotypes was performed using ten genomic simple sequence repeats (SSRs). A total of 50 genotypes from Tunisia including local landraces identified while prospecting the different sites of Bizerte and Sidi Bouzid (Northern and central parts) which are the most important locations of almond diversity in Tunisia were included. Analysis of molecular variance (AMOVA) was performed for 11 populations from these different regions and foreign countries to examine the distribution of genetic variation of the accessions studied. Results show that the major variation occurred within populations in each geographic site. Additionally, this analysis demonstrates that the genetic diversity within local almond cultivars was important, with a clear geographic distinction between the Northern and the Southern Tunisian cultivars. The value of prospecting new sites, preserving genetic diversity and encouraging on farmers almond collections is also discussed.

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“…The markers provide a very reliable and convenient tool for the analyzing genetic diversity of P. davidiana. Genetic diversity studies have been performed in peach and other species of the subgenus Amygdalus (Aranzana et al, ,2010Bouhadida et al, 2007;Cheng, 2007a,b;Cheng & Huang, 2009;Cipriani et al, 1999;Dirlewanger et al, 2002;Shiran, 2007;Sosinski et al, 2000;Testolin et al, 2000) however, there are no studies on the population structure, LD and selection of natural populations of P. davidiana.…”

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confidence: 99%

Population Structure, Linkage Disequilibrium and Selective Loci in Natural Populations of Prunus davidiana

Cheng¹,

Gašić²,

Wang³

2013

JAS

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Prunus davidiana (Carrie're) Franch is a very important resource for the restoration in dry and arid areas, genetic improvement of peach, and extraction of health-promoting components. To effectively use the resource, we must have a measure of genetic diversity of P. davidiana and its population structure. LD (Linkage disequilibrium) provides information for association mapping underlying the phenotypic variation observed. Selective loci reveal adaptive evolution processes resulting from natural selection. A set of 190 genotypes from seven natural populations（SXTB, SIYQ, SXFX, NXXJ, SIJC, GSHT, GSHS) of P. davidiana collected from the range of P. davidiana in China was fingerprinted with 23 SSR markers, and analyzed with spatial structure, pairwise Fst (differentiation coefficient), PCA (principal coordinate analysis), estimation of groups of populations with STRUCTURE software, selective loci obtained from lnRH tested by standardization distribution and Grubbs. Our results demonstrate that population structure of four groups existed among populations through complementary analyses of the above mentioned methods; significant LD numbers from 22 to 129 between loci within unstructured populations were detected; there were five selective loci in all populations and two common selective loci for local natural selection between populations. We should conserve four populations among seven populations; these selective loci may provide information for disclosing adaption evolution and candidate genes according to selective loci and alleles; LDs inform how to use them for association analysis.

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Molecular characterization and genetic relationship among almond cultivars assessed by RAPD and SSR markers

Cited by 70 publications

References 44 publications

KERAGAMAN GENETIK TETUA DAN ANAKAN DARI KEBUN BENIH SEMAI Acacia mangium GRUP D (AM004) DI SUMATERA SELATAN, INDONESIA

KERAGAMAN GENETIK TETUA DAN ANAKAN DARI KEBUN BENIH SEMAI Acacia mangium GRUP D (AM004) DI SUMATERA SELATAN, INDONESIA

Molecular variance of the Tunisian almond germplasm assessed by simple sequence repeat (SSR) markers

Population Structure, Linkage Disequilibrium and Selective Loci in Natural Populations of Prunus davidiana

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