Molecular characterization and genetic diversity in some Egyptian wheat (Triticum aestivum L.) using microsatellite markers

El-Fiki, Ayman; Adly, Mohamed

doi:10.5219/978

Cited by 5 publications

(2 citation statements)

References 41 publications

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“…al., (2016);ŽIAROVSKÁ et. al., (2017); ANSARI et al, (2018); EL-FIKI et al, (2019); ŠTIASNA et al, (2019). The present study is focused on estimation of genetic distance between 56 Tunisian castor genotypes, based on 20 SSR markers.…”

Section: Introductionmentioning

confidence: 96%

Genetic diversity and relationship of Tunisian castor (Ricinus communis L.) genotypes revealed by SSR markers

Vivodík¹,

Saadaoui²,

Balážová³

et al. 2020

Genetika

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The aim of this study was to assess genetic diversity within the set of 55 Tunisian castor genotypes using 20 SSR primers. PCR amplification of DNA using 20 primers for SSR analysis produced 141 DNA fragments that could be scored in all 55 genotypes of castor. The number of amplified fragments varied from 4 (Rco 15) to 10 (Rco 29 and Rco 33). Of the 141 amplified bands, all 141 were polymorphic, with an average of 7.05 polymorphic bands per primer. The polymorphism information content (PIC) value varied from 0.719 (Rco 15) to 0.879 (Rco 29), with an average of 0.825 and index diversity (DI) value varied from 0.745 (Rco 15) to 0.881 (Rco 29) with an average of 0.832. 100% of used SSR markers had PIC and DI values higher than 0.7 that means high polymorphism of chosen markers used for analysis. Probability of identity (PI) was low ranged from 0.002 (Rco 29 and Rco 41) to 0.018 (Rco 15) with an average of 0.007. A dendrogram based on UPGMA analysis separated 55 Tunisian castor genotypes into 4 clusters (1, 2, 3, 4). Knowledge on the genetic diversity of castor can be used for future breeding programs for increased oil production to meet the ever increasing demand of castor oil for industrial uses as well as for biodiesel production.

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Section: Introductionmentioning

confidence: 96%

Genetic diversity and relationship of Tunisian castor (Ricinus communis L.) genotypes revealed by SSR markers

Vivodík¹,

Saadaoui²,

Balážová³

et al. 2020

Genetika

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“…They are abundant and scattered all over the eukaryotic genomes with a high polymorphism rate (Kesawat and Kumar, 2009). Different studies have reported the use of SSRs to reveal polymorphisms in the wheat population (Khan et al 2015;Zarei., et al, 2016;Ya Narantsetseg et al, 2017;Salehi et al 2018;Yadav and Chand, 2018;El-Fiki and Adly, 2019). The results of these investigations indicated that wheat populations had high genetic diversity that can be used in wheat conservation and breeding programs, as well most SSR markers used showed a high level of polymorphism in wheat.…”

Section: Introductionmentioning

confidence: 97%

Assessment of Genetic Diversity and Population Structure of Some Soft and Hard Wheat Varieties Based on SSR Marker

Fattah

Tayib

2020

SJUOZ

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Wheat (Triticum spp.) is one of the most important cereal crops in Iraq and the world. It includes many species and varieties. The two major cultivated species of wheat are, durum wheat (Tritium durum Desf.) which is tetraploid (2n= 28) and the common wheat (Triticum aestivum L.) which is hexaploid (2n = 42). Ten wheat varieties from both species were examined using ten Simple sequence repeat (SSR) markers (WMC17, WMC20, WMC21, WMC24, WMC25, WMC48, WMC50, WMC283, Xgwm11 and Xgwm626). Various genetic parameters were calculated using Power Marker V3.25 software. A total of 156 alleles were detected in both species. The gene diversity in wheat varieties from both species collectively varied from 0.85 to 1.00, which indicates considerable genetic diversity in the examined varieties. All markers used in this study were highly informative and the polymorphic information content (PIC) values were higher than 0.50 in all loci. Hence all markers are considered useful for genetic diversity studies in wheat’s populations. The dendrogram separated the populations into two main clades and many subgroups. Azadi variety was simplicifolious. This study confirms the discriminating power of SSR typing and its usefulness for comparison within hard and soft wheat populations.

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Mutations induced in some Egyptian cowpea varieties with yield characteristics and high nutritional value using gamma rays and evaluation by microsatellite markers

Adly,

Ezzat,

Awad

et al. 2024

International Journal of Radiation Biology

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Molecular characterization and genetic diversity in some Egyptian wheat (Triticum aestivum L.) using microsatellite markers

Cited by 5 publications

References 41 publications

Genetic diversity and relationship of Tunisian castor (Ricinus communis L.) genotypes revealed by SSR markers

Genetic diversity and relationship of Tunisian castor (Ricinus communis L.) genotypes revealed by SSR markers

Assessment of Genetic Diversity and Population Structure of Some Soft and Hard Wheat Varieties Based on SSR Marker

Mutations induced in some Egyptian cowpea varieties with yield characteristics and high nutritional value using gamma rays and evaluation by microsatellite markers

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