Molecular Characterization and Expression Pattern of Fetuin-B in Gibel Carp (Carassius auratus gibelio)

Abstract

Fetuin-B has recently been cloned and identified from rats, mice, and humans; their expression patterns, however, have not been elucidated yet. In this study, Cagfetuin-B has been cloned in gibel carp. RT-PCR and Western blot detection revealed that Cagfetuin-B is first transcribed from the blastula stage and at a relatively stable level afterward during embryogenesis and the larval stage. Cagfetuin-B transcripts are predominantly distributed over the yolk syncytial layer in the early embryos and later restricted to the cells of liver and brain in newly hatched larvae. Moreover, a dynamic distribution of Cagfetuin-B protein was observed in brain, kidney, liver, and skin during morphogenesis. In adult fish, Cagfetuin-B transcripts are restricted in liver and ovary. Our work, for the first time, revealed the extra-hepatic transcription and a dynamic distribution of fetuin-B during embryogenesis and in adults, which indicates the potential roles of fetuin-B in fish organogenesis.

Molecular characterization and expression pattern of AFPIV during embryogenesis in gibel carp(Carassiu auratus gibelio)

“…And some important genes involved in embryogenesis, such as CagCNBP, Cagfetuin-B, CagApo-14 and CagMdkb have been identified in the model system [19][20][21][22]. In this study, CagAFPIV was cloned from gibel carp.…”

Expression pattern of cellular nucleic acid-binding protein (CNBP) during embryogenesis and spermatogenesis of gibel carp

“…And some important genes involved in embryogenesis and gametogenesis, such as CagApo-14, CagMdkb, CagCNBP, CagFetuin-B and CagVasa [10,[17][18][19] have been identified in the model system. In the present study, CagCNBP expression pattern was characterized in gibel carp embryos and larva.…”