Molecular characterization and expression pattern of AFPIV during embryogenesis in gibel carp(Carassiu auratus gibelio)

Liu, Jing‐Xia; Zhai, Yunfeng; Gui, Jian‐Fang

doi:10.1007/s11033-008-9412-3

Cited by 17 publications

(18 citation statements)

References 28 publications

(32 reference statements)

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“…AFPIV was first identified as an antifreeze protein in teleost blood plasma [14,15], and abundant expression was detected during carp embryogenesis [41] and in an Atlantic cod ovary EST library [23]. Prior research in cod, however, has found no antifreeze activity in eggs and larvae [69], and AFPIV function as an antifreeze protein is unclear, as it only renders low thermal hysteresis [22] and is structurally similar to apolipoproteins (lipid-binding proteins) from many fish species.…”

Section: Lipid Metabolismmentioning

confidence: 99%

Identification of ovarian gene expression patterns during vitellogenesis in Atlantic cod (Gadus morhua)

Breton

Anderson

Goetz

et al. 2012

General and Comparative Endocrinology

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Follicular maturational competence and ovulatory competence in teleost fish refer to the ability of the ovarian follicle to undergo final oocyte maturation and ovulation, respectively, in response to gonadotropin stimulation and other external cues. Some gene products related to competence acquisition are likely synthesized during vitellogenic growth, as these follicles gain in vivo responsiveness to exogenous gonadotropin stimulation and can be induced to undergo maturation and ovulation. In Atlantic cod (Gadus morhua), gonadotropin responsiveness has been shown to be oocyte size-dependent, and only ovaries containing late-stage vitellogenic follicles can be induced to ovulate. The purpose of the present study was to compare gene expression patterns between mid (unresponsive) and late (responsive) vitellogenic ovaries to identify genes involved in gonadotropin responsiveness and the acquisition of maturational and ovulatory competencies. Representational difference analysis was conducted in two reciprocal comparisons using intact ovarian fragments and follicle wall-enriched tissues, and genes of interest were used in real time quantitative PCR to confirm differential expression. Few differences were detected in intact ovarian fragments, but type IV ice-structuring protein and gephyrin were upregulated later in development and may be involved in lipid and sulfur metabolism, respectively. Candidate gene assays for luteinizing hormone receptor and aromatase also exhibited significant upregulation during vitellogenesis. Many genes were differentially expressed in follicle wall-enriched tissues, including endocrine maturational regulators and smooth muscle genes. Overall, maturational and ovulatory competencies during vitellogenesis in Atlantic cod are associated with up- and downregulation of many genes involved in lipid metabolism, endocrine regulation, and ovulatory preparation.

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Section: Lipid Metabolismmentioning

confidence: 99%

Identification of ovarian gene expression patterns during vitellogenesis in Atlantic cod (Gadus morhua)

Breton

Anderson

Goetz

et al. 2012

General and Comparative Endocrinology

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“…Following our systematic studies on maternal factor screening and gene function identification 9-13 in polyploid gibel carp ( Carassius auratus gibelio , Cag ) with multiple modes of unisexual and sexual reproduction 14-19, we have also identified and characterized a type-IV antifreeze protein gene ( afp4 ) 20. Antifreeze proteins (AFPs) have been found in fungi, bacteria, plants and animals, and they can bind to ice crystals to inhibit growth and recrystallization of ice 21.…”

Section: Introductionmentioning

confidence: 99%

“…According to their molecular structures, four types of AFPs, type I-IV AFPs, have been characterized in teleost fishes 22. The type-IV antifreeze protein (AFP4) was firstly discovered from longhorn sculpin ( Myoxocephalus octodecimspinosis ) in 1997 23, and its homologues had been identified in other teleost fishes, ranging from polar to tropic region and from seawater to freshwater 20, 24, 25. As a new type of AFP, their molecular structures have been characterized by the conserved four-helix bundle 24-26, and the antifreeze activity has been demonstrated in several fishes 23-26.…”

Section: Introductionmentioning

confidence: 99%

Type-IV Antifreeze Proteins are Essential for Epiboly and Convergence in Gastrulation of Zebrafish Embryos

Xiao¹,

Xia²,

Zhang³

et al. 2014

Int. J. Biol. Sci.

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Many organisms in extremely cold environments such as the Antarctic Pole have evolved antifreeze molecules to prevent ice formation. There are four types of antifreeze proteins (AFPs). Type-IV antifreeze proteins (AFP4s) are present also in certain temperate and even tropical fish, which has raised a question as to whether these AFP4s have important functions in addition to antifreeze activity. Here we report the identification and functional analyses of AFP4s in cyprinid fish. Two genes, namely afp4a and afp4b coding for AFP4s, were identified in gibel carp (Carassius auratus gibelio) and zebrafish (Danio rerio). In both species, afp4a and afp4b display a head-to-tail tandem arrangement and share a common 4-exonic gene structure. In zebrafish, both afp4a and afp4b were found to express specifically in the yolk syncytial layer (YSL). Interestingly, afp4a expression continues in YSL and digestive system from early embryos to adults, whereas afp4b expression is restricted to embryogenesis. Importantly, we have shown by using afp4a-specific and afp4b-specifc morpholino knockdown and cell lineage tracing approaches that AFP4a participates in epiboly progression by stabilizing yolk cytoplasmic layer microtubules, and AFP4b is primarily related to convergence movement. Therefore, both AFP4 proteins are essential for gastrulation of zebrafish embryos. Our current results provide first evidence that AFP such as AFP4 has important roles in regulating developmental processes besides its well-known function as antifreeze factors.

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“…Notably, if longhorn sculpin AFP IV had been evolved only through the selective pressure of the cold Antarctic environment approximately 10-15 million years ago, then there would likely be a very slim chance of comparable AFP IV evolution in temperate, subtropical, and/or tropical fish such as P. olivaceus, which might have experienced very recent temporal glaciation approximately 2.5 to 3 million years ago. Instead, the abundant expression of AFP IVs in carp and zebra fish observed in oocytes and embryos strongly support its association with early development (Goetz et al 2006;Liu et al 2009;Brenton et al 2012). …”

Section: Discussionmentioning

confidence: 97%

“…Based on the initial characterization of two AFP IVs from longhorn and shorthorn sculpins, Gauthier et al (2008) speculated that AFP IV may not have been selected and evolved as a main antifreeze owing to the presence of the more efficient type I AFP. In addition, very recently, a Chinese group reported that two AFP IV genes, afp4a and afp4b, from the tropical fish gibel carp (Carassius auratus gibelio) and zebrafish (Danio rerio) are essential for epiboly progression and convergent movement during zebrafish embryo gastrulation (Liu et al 2009;Lee et al 2011;Xiao et al 2014). This evidence is consistent with previous findings that some apolipoproteins, which are homologues of AFP IV, play a significant role in embryonic morphogenesis and organogenesis in these fish (Xia et al 2008;Choudhury et al 2009;Zhang et al 2011;Wang et al 2013;Xiao et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Cloning, expression, and activity of type IV antifreeze protein from cultured subtropical olive flounder (Paralichthys olivaceus)

Lee

Kim

2016

Fish Aquatic Sci

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Antifreeze proteins (AFPs) lower the freezing point but not the melting point of aqueous solutions by inhibiting the growth of ice crystals via an adsorption-inhibition mechanism. However, the function of type IV AFP (AFP IV) is questionable, as its antifreeze activity is on the verge of detectable limits, its physiological concentration in adult fish blood is too low to function as a biological antifreeze, and its homologues are present even in fish from tropic oceans as well as freshwater. Therefore, we speculated that AFP IV may have gained antifreeze activity not by selective pressure but by chance. To test this hypothesis, we cloned, expressed, and assayed AFP IV from cultured subtropical olive flounder (Paralichthys olivaceus), which do not require antifreeze protein for survival. Among the identified expressed sequence tags of the flounder liver sample, a 5′-deleted complementary DNA (cDNA) sequence similar to the afp4 gene of the longhorn sculpin was identified, and its full-length cDNA and genome structure were examined. The deduced amino acid sequence of flounder AFP IV shared 55, 53, 52, and 49 % identity with those of Pleuragramma antarcticum, Myoxocephalus octodecemspinosus, Myoxocephalus scorpius, and Notothenia coriiceps, respectively. Furthermore, the genomic structure of this gene was conserved with those of other known AFP IVs. Notably, the recombinant AFP IV showed a weak but distinct thermal hysteresis of 0.07 ± 0.01°C at the concentration of 0.5 mg/mL, and ice crystals in an AFP IV solution grew star-shaped, which are very similar to those obtained from other polar AFP IVs. Taken together, our results do not support the hypothesis of evolution of AFP IV by selective pressure, suggesting that the antifreeze activity of AFP IV may have been gained by chance.

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Molecular characterization and expression pattern of AFPIV during embryogenesis in gibel carp(Carassiu auratus gibelio)

Abstract: As a new type of AFPs,

Cited by 17 publications

References 28 publications

Identification of ovarian gene expression patterns during vitellogenesis in Atlantic cod (Gadus morhua)

Identification of ovarian gene expression patterns during vitellogenesis in Atlantic cod (Gadus morhua)

Type-IV Antifreeze Proteins are Essential for Epiboly and Convergence in Gastrulation of Zebrafish Embryos

Cloning, expression, and activity of type IV antifreeze protein from cultured subtropical olive flounder (Paralichthys olivaceus)

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