Isolation and identification of fetuin-B-like protein from rainbow trout seminal plasma and its localization in the reproductive system

Nynca, Joanna; Słowińska, Mariola; Dietrich, Mariola A.; Bilińska, Barbara; Kotula–Balak, Małgorzata; Ciereszko, Andrzej

doi:10.1016/j.cbpb.2010.10.002

Cited by 18 publications

(10 citation statements)

References 45 publications

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“…Although rainbow trout ( Oncorhynchus mykiss ) is one of the most important species in the world's aquaculture, the knowledge about seminal plasma proteins is limited to the identification of only a few proteins, such as α 1 ‐antiproteinase , fetuin‐B‐like protein , lipocalin‐type prostaglandin D synthase , and apolipoproteins (ApoC‐Ia,b,c) . These proteins were isolated and purified from seminal plasma using chromatographic methods followed by identification via Edman degradation.…”

Section: Proteins Identified In Rainbow Trout Seminal Plasma By Lc‐msmentioning

confidence: 99%

Proteomic identification of rainbow trout seminal plasma proteins

et al. 2013

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In the study, the combination of protein fractionation by 1DE and HPLC-ESI-MS/MS was used to characterize the rainbow trout seminal plasma proteome. Our results led to the creation of a catalogue of rainbow trout seminal plasma proteins (152 proteins) and significantly contributed to the current knowledge regarding the protein composition of fish seminal plasma. The major proteins of rainbow trout seminal plasma, such as transferrin, apolipoproteins, complement C3, serum albumin, and hemopexin-, alpha-1-antiproteinase-, and precerebellin-like protein, were recognized as acute-phase proteins (proteins that plasma concentration changes in response to inflammation). This study provides the basis for further functional studies of fish seminal plasma proteins, as well as for the identification of novel biomarkers for sperm quality. The MS data have been deposited in the ProteomeXchange with identifier PXD000306 (http://proteomecentral.proteomexchange.org/dataset/PXD000306).

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Section: Proteins Identified In Rainbow Trout Seminal Plasma By Lc‐msmentioning

confidence: 99%

Proteomic identification of rainbow trout seminal plasma proteins

et al. 2013

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“…This is in agreement with previous data indicating that most rainbow trout seminal proteins are antigenically related to serum proteins . Moreover, these findings were confirmed in our studies of individual major proteins in trout SP, including protease inhibitors (α 1 ‐antiproteinase and fetuin‐B‐like protein) and TF . Those proteins are suggested to be transported to the semen from the blood and/or partially synthesised and secreted by reproductive tract cells, since their immunohistochemical staining was found in reproductive tissues (within the testis in Sertoli and Leydig cells, as well as in the efferent duct) and liver.…”

Section: Discussionmentioning

confidence: 99%

“…The Western blot was performed as described by Nynca et al. . Polyclonal antibodies against TF and PGDS were diluted with TBS‐T at ratios of 1:40 000 and 1:3000, respectively.…”

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Proteomic identification of rainbow trout blood plasma proteins and their relationship to seminal plasma proteins

et al. 2017

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The characterisation of fish blood proteomes is important for comparative studies of seminal and blood proteins as well as for the analysis of fish immune mechanisms and pathways. In this study, LC-MS/MS and 2D-DIGE were applied to compare rainbow trout seminal (SP) and blood plasma (BP) proteomes. The 54 differentially abundant proteins identified in SP are involved in a variety of signalling pathways, including protein ubiquitination, liver X receptor/retinoid X receptor (LXR/RXR) and farnesoid X receptor activation, cell cycle and acute phase signalling. These findings may indicate the prevalence of acute phase signalling pathways in trout SP, and its essential role in protecting spermatozoa and reproductive tissues. Our study provides the first in-depth analysis of the trout BP proteome, with a total of 119 proteins identified. The major proteins of rainbow trout BP were recognised as acute phase proteins. Analysis of BP proteins indicated that acute phase response signalling, the complement system, liver X receptor/retinoid X receptor and farnesoid X receptor activation and the coagulation system are the top canonical pathways. This study enhances knowledge of the blood origin of trout SP proteins and understanding of fish reproductive biology. Our results provide new insight into blood proteins specifically important for fish physiology and innate immunity. The mass spectrometry data are available via ProteomeXchange with the identifier PXD005988 and https://doi.org/10.6019/PXD005988.

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“…We have focused on the most abundant (major) proteins that are already visible through protein staining after one‐dimensional electrophoresis. For rainbow trout seminal plasma, the following proteins have been identified: a serpin of α 1 ‐antitrypsin type (Mak et al., ), apolipoproteins C‐I (ApoC‐Ia, Ib, Ic; Nynca et al., ), fetuin‐B‐like protein (Nynca et al., ) and lipocalin‐type protein (Nynca et al., ). For common carp seminal plasma the following proteins have been identified: a serpin of α 1 ‐antitrypsin type (Wojtczak et al., ), transferrin (Wojtczak et al., ; Dietrich et al., ), and parvalbumin (Dietrich et al., ).…”

Section: The Identification Of Fish Seminal Proteins Using a Traditiomentioning

confidence: 99%

The identification of seminal proteins in fish: from a traditional approach to proteomics

2012

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Summary This contribution presents an overview of the potential capabilities of evolving methodologies to characterize seminal protein profiles and identify interactions while simultaneously analyzing the complex but comprehensive protein matrix. The traditional approach to protein characterization in fish semen is based on two major steps: the purification of proteins using numerous liquid preparative chromatographic methods and the identification of proteins using sequencing. In using this approach major proteins of fish seminal plasma were identified, including apolipoprotein A‐I, transferrin, α1 – antiproteinase and parvalbumins for carp; and fetuin B‐like protein, α1 – antiproteinase, lipocalin‐type protein and apolipoprotein C‐I for rainbow trout. Proteomics provides for a great advance in protein studies. Proteomics is concerned with the study of protein products expressed by the genome, with the term ‘proteome’ originating from PROTEin complement of the genOME. Proteomics emerged as a leading technology in the post‐genomic era because of the central role of proteins and protein–protein interactions in cell physiology. The first applications of proteomic research in the study of fish reproduction have already been made, and include the effects of cryopreservation on semen proteome, molecular mechanisms determining sperm motility initiation and the effects of domestication of cultured species on semen quality. The introduction of proteomics in the study of male reproduction in fish creates a unique opportunity to unravel, using an integrative approach, the physiological mechanisms important for sperm function, such as motility and fertilizing ability. It is anticipated that proteomic studies will greatly increase the chances for reliably identifying new biomarkers in male fertility studies. However, a better understanding on the opportunities and limitations of the new disciplines or subject areas are required.

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Isolation and identification of fetuin-B-like protein from rainbow trout seminal plasma and its localization in the reproductive system

Cited by 18 publications

References 45 publications

Proteomic identification of rainbow trout seminal plasma proteins

Proteomic identification of rainbow trout seminal plasma proteins

Proteomic identification of rainbow trout blood plasma proteins and their relationship to seminal plasma proteins

The identification of seminal proteins in fish: from a traditional approach to proteomics

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