Molecular, Biochemical, and Electrophysiological Characterization of Drosophila norpA Mutants

Pearn, Michael T.; Randall, Lydia L.; Shortridge, Randall D.; Burg, Martin G.; Pak, William L.

doi:10.1074/jbc.271.9.4937

Cited by 136 publications

(44 citation statements)

References 50 publications

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“…In contrast, the optic lobes are widely labelled but a role for visual information in the female in this behavioural context has not been reported. To investigate whether the inability to process visual stimuli could explain the reduced mating, we tested the no receptor potential A (norpA ) mutant flies, that have no phototransduction43. We observed that the mating of these flies is not changed (Figure S1c).…”

Section: Resultsmentioning

confidence: 99%

“…Fly strains and sources are as follows: ap md544 60; UAS - Kir2 .140; tub-GAL80 TS 41; UAS-CD8-GFP 61; 20xUAS-CD8-GFP 62; UAS-AUG-DsRed (Kasuya & Iverson, Flybase); Otd-nls:FLPo 44, provided by D. Anderson ; UAS > stop > CD8-GFP 63 ; UAS > stop > Kir2 20, provided by Y.N. Jan ; fru LexA 52, provided by D. Mellert ; LexAop-CD2-GFP 64; 8xLexAop2-FLP L 65; UAS-dcr-2 66; UAS-tra-2 IR 66; elav-GAL80 20; repo-GAL80 67, provided by T. Lee ; OK107-GAL4 68; norpA 3643; tub > stop > GAL80 45; actin5C-GAL4 69; VGlut MI04979 -LexA:QFAD, GAD1 MI09277 -LexA:QFAD, Cha MI04508 -LexA:QFAD 70. Wild-type: Canton-S (CS) and Dickinson Lab (DL).…”

Section: Methodsmentioning

confidence: 99%

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apterous Brain Neurons Control Receptivity to Male Courtship in Drosophila Melanogaster Females

Aranha

Herrmann

Cachitas

et al. 2017

Sci Rep

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Courtship behaviours allow animals to interact and display their qualities before committing to reproduction. In fly courtship, the female decides whether or not to mate and is thought to display receptivity by slowing down to accept the male. Very little is known on the neuronal brain circuitry controlling female receptivity. Here we use genetic manipulation and behavioural studies to identify a novel set of neurons in the brain that controls sexual receptivity in the female without triggering the postmating response. We show that these neurons, defined by the expression of the transcription factor apterous, affect the modulation of female walking speed during courtship. Interestingly, we found that the apterous neurons required for female receptivity are neither doublesex nor fruitless positive suggesting that apterous neurons are not specified by the sex-determination cascade. Overall, these findings identify a neuronal substrate underlying female response to courtship and highlight the central role of walking speed in the receptivity behaviour.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

apterous Brain Neurons Control Receptivity to Male Courtship in Drosophila Melanogaster Females

Aranha

Herrmann

Cachitas

et al. 2017

Sci Rep

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“…The predicted mutant protein therefore interrupts the catalytic domain (α/β-barrel, extending from residue 318–682) and lacks the C-terminal tail required for Gq protein interaction and localization of PLC-β to the Rhabdomere (Katan, 1998). In contrast, the other frequently used null allele norpA P24 (or norpA 36 ) carries a 28-bp deletion within exon 10, which results in the absence of the same C-terminal domains, except for retaining the complete catalytic domain (Pearn et al, 1996) (Fig. 5).…”

Section: Resultsmentioning

confidence: 99%

Rhodopsin 5– and Rhodopsin 6–Mediated Clock Synchronization in Drosophila melanogaster Is Independent of Retinal Phospholipase C-β Signaling

et al. 2012

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Circadian clocks of most organisms are synchronized with the 24-hour solar day by the changes of light and dark. In Drosophila, both the visual photoreceptors in the compound eyes as well as the blue-light photoreceptor Cryptochrome expressed within the brain clock neurons contribute to this clock synchronization. A specialized photoreceptive structure located between the retina and the optic lobes, the Hofbauer-Buchner (H-B) eyelet, projects to the clock neurons in the brain and also participates in light synchronization. The compound eye photoreceptors and the H-B eyelet contain Rhodopsin photopigments, which activate the canonical invertebrate phototransduction cascade after being excited by light. We show here that 2 of the photopigments present in these photoreceptors, Rhodopsin 5 (Rh5) and Rhodopsin 6 (Rh6), contribute to light synchronization in a mutant (norpAP41) that disrupts canonical phototransduction due to the absence of Phospholipase C-β (PLC-β). We reveal that norpAP41 is a true loss-of-function allele, resulting in a truncated PLC-β protein that lacks the catalytic domain. Light reception mediated by Rh5 and Rh6 must therefore utilize either a different (nonretinal) PLC-β enzyme or alternative signaling mechanisms, at least in terms of clock-relevant photoreception. This novel signaling mode may distinguish Rhodopsin-mediated irradiance detection from image-forming vision in Drosophila.

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“…Loss of external eyes and ocelli or eliminating opsin-based photoreception via vitamin A depletion or phototransduction mutants reduces light sensitivity of the clock but doesn’t abolish entrainment of locomotor activity rhythms by light (Blaschke et al, 1996; Hu et al, 1978; Ohata et al, 1998; Pearn et al, 1996), which suggests that other photoreceptors function to entrain circadian oscillators in brain pacemaker cells. A screen for mutants that disrupted rhythms in bioluminescence produced by per -luc identified CRYPTOCHROME (CRY) (Emery et al, 1998; Stanewsky et al, 1998), which is an ortholog of blue light photoreceptor cryptochromes in plants (Ahmad and Cashmore, 1993; Lin et al, 1998).…”

Section: Light Entrainment Of the Drosophila Circadian Feedback Lmentioning

confidence: 99%

Molecular Genetic Analysis of Circadian Timekeeping in Drosophila

Hardin

2011

The Genetics of Circadian Rhythms

317

375

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A genetic screen for mutants that alter circadian rhythms in Drosophila identified the first clock gene - the period (per) gene. The per gene is a central player within a transcriptional feedback loop that represents the core mechanism for keeping circadian time in Drosophila and other animals. The per feedback loop, or core loop, is interlocked with the Clock (Clk) feedback loop, but whether the Clk feedback loop contributes to circadian timekeeping is not known. A series of distinct molecular events are thought to control transcriptional feedback in the core loop. The time it takes to complete these events should take much less than 24h, thus delays must be imposed at different steps within the core loop. As new clock genes are identified, the molecular mechanisms responsible for these delays have been revealed in ever-increasing detail, and provide an in depth accounting of how transcriptional feedback loops keep circadian time. The phase of these feedback loops shift to maintain synchrony with environmental cycles, the most reliable of which is light. Although a great deal is known about cell-autonomous mechanisms of light-induced phase shifting by CRYPTOCHROME (CRY), much less is known about non-cell autonomous mechanisms. CRY mediates phase shifts through an uncharacterized mechanism in certain brain oscillator neurons, and carries out a dual role as a photoreceptor and transcription factor in other tissues. Here I will review how transcriptional feedback loops function to keep time in Drosophila, how they impose delays to maintain a 24h cycle, and how they maintain synchrony with environmental light:dark cycles. The transcriptional feedback loops that keep time in Drosophila are well conserved in other animals, thus what we learn about these loops in Drosophila should continue to provide insight into the operation of analogous transcriptional feedback loops in other animals.

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Molecular, Biochemical, and Electrophysiological Characterization of Drosophila norpA Mutants

Cited by 136 publications

References 50 publications

apterous Brain Neurons Control Receptivity to Male Courtship in Drosophila Melanogaster Females

apterous Brain Neurons Control Receptivity to Male Courtship in Drosophila Melanogaster Females

Rhodopsin 5– and Rhodopsin 6–Mediated Clock Synchronization in Drosophila melanogaster Is Independent of Retinal Phospholipase C-β Signaling

Molecular Genetic Analysis of Circadian Timekeeping in Drosophila

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