apterous Brain Neurons Control Receptivity to Male Courtship in Drosophila Melanogaster Females

Aranha, Márcia M.; Herrmann, Dennis; Cachitas, Hugo; Neto-Silva, Ricardo M.; Dias, Sophie; Vasconcelos, Maria Luísa

doi:10.1038/srep46242

Cited by 24 publications

(16 citation statements)

References 71 publications

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“…It has previously been reported that Apterous -GAL4 (Apt-GAL4) drives expression in the LHLK neurons, as well as neurons in the optic lobe and antennal mechanosensory and motor centers (AMMCs), and a small population of mushroom-body neurons (S2A Fig) [27,32]. Immunostaining with Lk antibody in Apt-GAL4>UAS-mCD8::GFP flies confirmed colocalization exclusively within the LHLK neurons (S2A and S2B Fig).…”

Section: Resultsmentioning

confidence: 78%

A single pair of leucokinin neurons are modulated by feeding state and regulate sleep–metabolism interactions

et al. 2019

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Dysregulation of sleep and feeding has widespread health consequences. Despite extensive epidemiological evidence for interactions between sleep and metabolic function, little is known about the neural or molecular basis underlying the integration of these processes. D . melanogaster potently suppress sleep in response to starvation, and powerful genetic tools allow for mechanistic investigation of sleep–metabolism interactions. We have previously identified neurons expressing the neuropeptide leucokinin (Lk) as being required for starvation-mediated changes in sleep. Here, we demonstrate an essential role for Lk neuropeptide in metabolic regulation of sleep. The activity of Lk neurons is modulated by feeding, with reduced activity in response to glucose and increased activity under starvation conditions. Both genetic silencing and laser-mediated microablation localize Lk-dependent sleep regulation to a single pair of Lk neurons within the Lateral Horn (LHLK neurons). A targeted screen identified a role for 5′ adenosine monophosphate-activated protein kinase (AMPK) in starvation-modulated changes in sleep. Knockdown of AMPK in Lk neurons suppresses sleep and increases LHLK neuron activity in fed flies, phenocopying the starvation state. Further, we find a requirement for the Lk receptor in the insulin-producing cells (IPCs), suggesting LHLK–IPC connectivity is critical for sleep regulation under starved conditions. Taken together, these findings localize feeding-state–dependent regulation of sleep to a single pair of neurons within the fruit fly brain and provide a system for investigating the cellular basis of sleep–metabolism interactions.

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Section: Resultsmentioning

confidence: 78%

A single pair of leucokinin neurons are modulated by feeding state and regulate sleep–metabolism interactions

et al. 2019

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“…; Aranha et al. ), there is much potential to use this genetic model to gain access into the neuronal architecture of female control over paternity. Moreover, the propensity of D. melanogaster females to remate is not only influenced by food availability (Gorter et al.…”

Section: Discussionmentioning

confidence: 99%

Last male sperm precedence is modulated by female remating rate inDrosophila melanogaster

2018

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Following multiple matings, sperm from different males compete for fertilization within the female reproductive tract. In many species, this competition results in an unequal sharing of paternity that favors the most recent mate, termed last male sperm precedence (LMSP). Much of our understanding of LMSP comes from studies in Drosophila melanogaster that focus on twice‐mated females with standardized latencies between successive matings. Despite accumulating evidence indicating that females often mate with more than two males and exhibit variation in the latency between matings, the consequences of mating rate on LMSP are poorly understood. Here, we developed a paradigm utilizing D. melanogaster in which females remated at various time intervals with either two or three transgenic males that produce fluorescent sperm (green, red, or blue). This genetic manipulation enables paternity assessment of offspring and male‐specific sperm fate examination in female reproductive tracts. We found that remating latency had no relationship with LMSP in females that mated with two males. However, LMSP was significantly reduced in thrice‐mated females with short remating intervals; coinciding with reduced last‐male sperm storage. Thus, female remating rate influences the relative share of paternity, the overall clutch paternity diversity, and ultimately the acquisition of indirect genetic benefits to potentially maximize female reproductive success.

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“…It has previously been reported that Apterous -GAL4 drives expression in the LHLK neurons, as well as neurons in the optic lobe, antennal mechanosensory and motor centers (AMMC), and a small population of mushroom body neurons (S2A Fig) [25,30]. Immunostaining with Lk antibody in Apterous -GAL4>UAS-CD8::GFP flies revealed co-localization exclusively localized to the LHLK neurons (S2A and S2B Fig).…”

Section: Resultsmentioning

confidence: 86%

A single pair of leucokinin neurons are modulated by feeding state and regulate sleep-metabolism interactions

Yurgel

Kakad

Zandawala

et al. 2018

Preprint

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1Dysregulation of sleep and feeding has widespread health consequences. Despite 2 extensive epidemiological evidence for interactions between sleep and metabolic 3 function, little is known about the neural or molecular basis underlying the integration of 4 these processes. Drosophila melanogaster potently suppress sleep in response to 5 starvation, and powerful genetic tools allow for mechanistic investigation of sleep-6 metabolism interactions. We have previously identified neurons expressing the 7 neuropeptide leucokinin (Lk) as being required for starvation-mediated changes in sleep. 8Here, we demonstrate an essential role for Lk neuropeptide in metabolic regulation of 9 sleep. Further, we find that the activity of Lk neurons is modulated by feeding state and 10 circulating nutrients, with reduced activity in response to glucose and increased activity 11 under starvation conditions. Both genetic silencing and laser-mediated microablation 12 localize Lk-mediated sleep regulation to a single pair of Lk neurons within the lateral 13 horn (LHLK) that project near primary sleep and metabolic centers of the brain. A 14 targeted screen identified a critical role for AMP-activated protein kinase (AMPK) in 15 starvation-modulated changes in sleep. Disruption of AMPK function in Lk neurons 16 suppresses sleep and increases LHLK activity in fed flies, phenocopying the starvation 17 state. Taken together, these findings localize feeding state-dependent regulation of 18 sleep to a single pair of neurons within the fruit fly brain and provide a system for 19 investigating the cellular basis of sleep-metabolism interactions. 20 21 Introduction 1 Dysregulation of sleep and feeding has widespread health consequences and reciprocal 2 interactions between these processes underlie a number of pathologies [1-4]. Sleep loss 3 correlates with increased appetite and insulin insensitivity, while short-sleeping 4 individuals are more likely to develop obesity, metabolic syndrome, type II diabetes, and 5 cardiovascular disease [1,3,4]. Although the neural basis for sleep regulation has been 6 studied in detail, little is known about how feeding state and changes in metabolic 7 function modulate sleep [5,6]. Understanding how sleep and feeding states are 8 integrated may provide novel insights into the co-morbidity of disorders linked to sleep 9 and metabolic regulation.10 Animals balance nutritional state and energy expenditure in order to achieve metabolic 11 homeostasis [7,8]. In both flies and mammals, diet potently affects sleep regulation, 12 strengthening the idea that sleep and metabolic state interact [5,6,9]. Starvation leads to 13 sleep loss, or disrupted sleep architecture, presumably to induce foraging behavior, 14 while high-calorie diets have complex effects on sleep depending on macronutrient 15 content [10-13]. Behavioral and physiological responses to changes in feeding state are 16 modulated both by cell autonomous nutrient centers in the brain that sense changes in 17 circulating nutrients and through communication b...

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apterous Brain Neurons Control Receptivity to Male Courtship in Drosophila Melanogaster Females

Cited by 24 publications

References 71 publications

A single pair of leucokinin neurons are modulated by feeding state and regulate sleep–metabolism interactions

A single pair of leucokinin neurons are modulated by feeding state and regulate sleep–metabolism interactions

Last male sperm precedence is modulated by female remating rate inDrosophila melanogaster

A single pair of leucokinin neurons are modulated by feeding state and regulate sleep-metabolism interactions

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