Molecular and serological detection and of Toxoplasma gondii in small ruminants of southwest Iran and the potential risks for consumers

Yousefvand, Amin; Mirhosseini, Seyed Ali; Ghorbani, Masoud; Mohammadzadeh, Tahereh; Moghaddam, Mehrdad Moosazadeh; Mohammadyari, Shirin

doi:10.1007/s00003-020-01306-w

Cited by 14 publications

(10 citation statements)

References 64 publications

(59 reference statements)

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“…A higher percentage of positive samples was found using PCR (performed in different matrices) than ELISA (on serum samples). This agrees with other studies where a higher T. gondii prevalence was found through PCR compared to serological methods (Rasti et al 2017;Yousefvand et al 2021). Such differences most likely result from molecular methods having a higher precision, sensitivity, and specificity than serological methods (Martínez-Flores et al 2017;Abd El-Razik et al 2018).…”

Section: Discussionsupporting

confidence: 93%

“…In addition to skeletal and cardiac muscles, the central nervous system has been proven to be a preference site for tissue cysts (Tenter 2009). However, the distribution of cysts and the parasite burden in different tissues may depend on the T. gondii strain, the infective stage (oocysts, tachyzoites and bradyzoites), and the time of infection, increasing in heart and skeletal muscles over time (Dadimoghaddam et al 2014;Juránková et al 2014;Swierzy et al 2014;Yousefvand et al 2021).…”

Section: Discussionmentioning

confidence: 99%

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A survey on Apicomplexa protozoa in sheep slaughtered for human consumption

et al. 2022

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Infections with the Apicomplexa Toxoplasma gondii, Neospora caninum, and Sarcocystis spp. are common causes of reproductive disorders in sheep. However, few epidemiological studies regarding co-infections with these three protozoa are reported in sheep in Italy. For this reason, this study aims to evaluate possible co-infections with T. gondii, N. caninum, and Sarcocystis spp. in sheep slaughtered for human consumption. From April to July 2019, individual blood, brain, heart, and diaphragm samples were collected from 138 sheep after slaughtering. The presence of IgG anti-Toxoplasma in serum samples was evaluated through ELISA. DNA of the three protozoa was investigated using specific PCRs. Co-infection with T. gondii, N. caninum and Sarcocystis spp. was found in 66.7% of the examined sheep. Antibodies against T. gondii were found in the 36.2% of serum samples. The presence of T. gondii DNA was detected in the 67.4%, 77.5%, and 21.7% of the brain, heart, and diaphragm samples, respectively. Neospora caninum DNA was found in 72.5% of the examined brain samples. Sarcocystis spp. DNA was detected in 92% and 52.2% of the heart and diaphragm samples, respectively. Sequence analysis of the Sarcocystis spp. revealed the sole presence of Sarcocystis tenella. The present study demonstrates that sheep have a high risk of infection with the three Apicomplexa investigated, suggesting the need to adopt adequate measures to prevent the spread of these parasitic infections considering their clinical and economic impact on ovine production. Furthermore, the possible role sheep play in the zoonotic transmission of toxoplasmosis to humans was highlighted.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

A survey on Apicomplexa protozoa in sheep slaughtered for human consumption

et al. 2022

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“…varía entre los países según los hábitos alimentarios locales y la tasa de infección en los animales productores 9 , 37 . Los porcentajes de detección del parásito también pueden variar porque la carga parasitaria es un factor importante para detectar con métodos moleculares los protozoos en muestras de tejido 38 . En Colombia, se ha encontrado que la carne contaminada puede ser la fuente de infección en el 25 % de los casos de toxoplasmosis durante el embarazo 58 .…”

Section: Discussionunclassified

“…A nivel mundial, los rangos de prevalencia de T. gondii varían ampliamente dependiendo de la muestra, las condiciones biosanitarias, los hábitos o costumbres de la zona y la metodología de detección empleada, entre otros 9 , 37 , 38 . Pérez-Grisales, et al .…”

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Detección molecular de Toxoplasma gondii en carnes para consumo humano en Ibagué, Colombia

et al. 2022

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Introducción. Toxoplasma gondii es un parásito con gran potencial zoonótico que puede infectar un amplio rango de huéspedes de sangre caliente, incluidos los animales del sector pecuario, lo que causa pérdidas a la industria. En el humano, es patógeno en personas inmunosuprimidas y afecta el desarrollo del feto en infecciones congénitas. Además, se asocia con diversos trastornos del comportamiento en personas sanas. El humano puede adquirir T. gondii al consumir carnes contaminadas mal cocidas.Objetivo. Determinar la positividad de T. gondii en carnes de consumo humano (res, pollo y cerdo) en Ibagué, Colombia.Materiales y métodos. Se utilizó la PCR convencional anidada y la secuencia del gen B1 de T. gondii como blanco de amplificación. Se tomaron 186 muestras de carne comercializada en la zona urbana de Ibagué (62 de res, 62 de pollo y 62 de cerdo) y se obtuvo el porcentaje de positividad en cada tipo de carne evaluada.Resultados. Se encontró un porcentaje de positividad de 18,8 % en las muestras, siendo la carne de cerdo la del mayor porcentaje (22,5 %; 14/62), seguida por las muestras de carne de res (19,3 %; 12/62) y de pollo (14,5 %; 9/62). Los mejores productos amplificados fueron secuenciados en Macrogen, y alineados con las secuencias del gen B1 depositadas en el GenBank, con lo que se confirmó su identidad.Conclusiones. Este es el primer estudio sobre prevalencia de T. gondii en carnes para consumo humano en Ibagué y el departamento del Tolima. Se demostró que los tres tipos de carne representan un riesgo para la infección en humanos a nivel local.

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“…Sheep and goats present T. gondii in their consumable organs [ 89 ]. Serological and molecular methods have demonstrated that around of 10–24% of liver, meat, and heart samples were positive for sheep and goat [ 89 ].…”

Section: Prevalence Of Toxoplasma Gondii In Food P...mentioning

confidence: 99%

Toxoplasma gondii in Foods: Prevalence, Control, and Safety

Marín-García

Planas

Llobat

2022

Foods

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Toxoplasma gondii is an obligate intracellular parasite that causes toxoplasmosis, with approximately one third of the population around the world seropositive. The consumption of contaminated food is the main source of infection. These include meat products with T. gondii tissue cysts, and dairy products with tachyzoites. Recently, contamination has been detected in fresh products with oocysts and marine products. Despite the great health problems that are caused by T. gondii, currently there are no standardized methods for its detection in the food industry. In this review, we analyze the current detection methods, the prevalence of T. gondii in different food products, and the control measures. The main detection methods are bioassays, cell culture, molecular and microscopic techniques, and serological methods, but some of these do not have applicability in the food industry. As a result, emerging techniques are being developed that are aimed at the detection of multiple parasites simultaneously that would make their application more efficient in the industry. Since the prevalence of this parasite is high in many products (meat and milk, marine products, and vegetables), it is necessary to standardize detection methods, as well as implement control measures.

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Molecular and serological detection and of Toxoplasma gondii in small ruminants of southwest Iran and the potential risks for consumers

Cited by 14 publications

References 64 publications

A survey on Apicomplexa protozoa in sheep slaughtered for human consumption

A survey on Apicomplexa protozoa in sheep slaughtered for human consumption

Detección molecular de Toxoplasma gondii en carnes para consumo humano en Ibagué, Colombia

Toxoplasma gondii in Foods: Prevalence, Control, and Safety

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