Molecular and phylogenetic analysis of bovine coronavirus based on the spike glycoprotein gene

Martínez, Nadia; Brandão, Paulo Eduardo; Souza, Samilly Evangelista; Barrera, Maritza; Santana, N. F. C.; Arce, Heidy Díaz de; Pérez, Lester J.

doi:10.1016/j.meegid.2012.05.007

Cited by 31 publications

(43 citation statements)

References 46 publications

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“…In addition, all the Argentinean strains sequenced grouped separately from Mebus reference strain, which has been used for systematic vaccination of Argentinean cattle (Dr. Parreño, personal communication). Furthermore, Argentinean strains form a well-defined cluster, separate of the other geographical clusters like most of the Brazilian strains as was previously observed (Bidokhti et al, 2012;Chung et al, 2011;Fulton et al, 2013;Martinez et al, 2012;Takiuchi et al, 2008). The length of the branch indicates Argentinean cluster divergence from all the other groups of BCoVs.…”

Section: Whiɵn a European Strains Bsupporting

confidence: 71%

“…In contrast, little epidemiological information is available regarding BCoV detection, incidence and characterization in cattle from Central and South American countries. In Cuba, BCoV sequences clustered with BCoV strains from USA, suggesting a common origin for these viruses (Martinez et al, 2012). In South America, most of the information comes from studies conducted in Brazil (Asano et al, 2010;Barros et al, 2013;Brandao et al, 2008Brandao et al, , 2006Takiuchi et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Molecular and antigenic characterization of bovine Coronavirus circulating in Argentinean cattle during 1994–2010

Bok

Miño

Rodriguez

et al. 2015

Veterinary Microbiology

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Bovine coronavirus (BCoV) is an important viral pathogen associated with neonatal calf diarrhea. Our aim was to investigate the incidence of BCoV in diarrhea outbreaks in beef and dairy herds from Argentina during 1994-2010. A total of 5.365 fecal samples from diarrheic calves were screened for BCoV diagnosis by ELISA. The virus was detected in 1.71% (92/5365) of the samples corresponding to 5.95% (63/1058) of the diarrhea cases in 239 beef and 324 dairy farms. The detection rate of BCoV was significantly higher in dairy than in beef herds: 12.13% (29/239) vs. 4.32% (14/324) respectively. Phylogenetic analysis of the hypervariable S1 region of seven representative samples (from different husbandry systems, farm locations and years of sampling) indicated that BCoV strains circulating in Argentinean beef and dairy herds formed a cluster distinct from other geographical regions. Interestingly, Argentinean strains are distantly related (at both the nucleotide and amino acid levels) with the Mebus historic reference BCoV strain included in the vaccines currently available in Argentina. However, Mebus-induced antibodies were capable of neutralizing the BCoV Arg95, a field strain adapted to grow in vitro, and vice versa, indicating that both strains belong to the same CoV serotype reported in cattle. This work represents the first large survey describing BCoV circulation in Argentinean cattle.

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Section: Whiɵn a European Strains Bsupporting

confidence: 71%

Section: Introductionmentioning

confidence: 99%

Molecular and antigenic characterization of bovine Coronavirus circulating in Argentinean cattle during 1994–2010

Bok

Miño

Rodriguez

et al. 2015

Veterinary Microbiology

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“…To determine the genomic sequence of nsp12, S, and, N genes, a set of 17 overlapping RT-PCR products, from 553 to 1260 nucleotides, were generated using a total of 42 primers (Lau et al, 2011;Hasoksuz et al, 2002;Martínez et al, 2012). RT-PCR reactions were performed using One Step RT-PCR System (Qiagen, Holden, Germany), from 2.5 μl of nucleic acid in a final volume of 25 μl, according the manufacturer's instructions.…”

Section: Sequencing Of the Three Complete Genes Nsp12 S And N And Gmentioning

confidence: 99%

“…An additional maximum likelihood tree based on 143 complete sequences of S gene of BCoV, BCoV-like, and HCoV-OC43 from USA, Brazil, South Korea, China, Italy, Swedish, Denmark and France was constructed. To this end, 81S gene sequences from BCoVs and BCoVs-like were added to the previous S gene alignment (Martínez et al, 2012;Beaudeau et al, 2010;Decaro et al, 2009a;Jeong et al, 2005;Zhang et al, 1991;Park et al, 2007;Chung et al, 2011;Park et al, 2006). The accession numbers corresponding to these sequences are provided in Tables S2 and S3 (supplementary material).…”

Section: Bioinformatic Analysismentioning

confidence: 99%

Comparative molecular epidemiology of two closely related coronaviruses, bovine coronavirus (BCoV) and human coronavirus OC43 (HCoV-OC43), reveals a different evolutionary pattern

Kin

Miszczak

Diancourt

et al. 2016

Infection, Genetics and Evolution

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Bovine coronaviruses (BCoVs) are widespread around the world and cause enteric or respiratory infections among cattle. The current study includes 13 samples from BCoVs collected in Normandy during an 11-year period (from 2003 to 2014), 16 French HCoV-OC43s, and 113 BCoVs or BCoVs-like sequence data derived from partial or complete genome sequences available on GenBank. According to a genotyping method developed previously for HCoV-OC43, BCoVs and BCoVs-like are distributed on three main sub-clusters named C1, C2, and C3. Sub-cluster C1 includes BCoVs and BCoVs-like from America and Asia. Sub-cluster C2 includes BCoVs from Europe. Sub-cluster C3 includes prototype, vaccine, or attenuated BCoV strains. The phylogenetic analyses revealed the monophyletic status of the BCoVs from France reported here for the first time. Moreover, BCoV exhibits a relative genetic stability when compared to HCoV-OC43 we previously described from the same region. The numerous recombination detected between HCoV-OC43 were much less frequent for BCoV. The analysis points thus to the influence of different evolutive constraints in these two close groups.

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“…Thus, the unique evolution of BCoV in yaks may be related to the special geographical environment of the Qinghai-Tibet Plateaui.e. the high-altitude (average altitude of over 4000 m), low oxygen, low temperature and low atmospheric pressurewhere the yak live, as well as to the characteristics of the host species [28][29][30][31].…”

Section: Prevalence Of Bcov In Yaks In Chinamentioning

confidence: 99%

First detection of bovine coronavirus in Yak (Bos grunniens) and a bovine coronavirus genome with a recombinant HE gene

Guo

Zhang

et al. 2019

Journal of General Virology

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The yak (Bosgrunniens) is a unique domestic bovine species that plays an indispensable role for herdsmen in the Qinghai-Tibet Plateau. Here, 336 diarrhoeic samples were collected from yaks on 29 farms in the Qinghai-Tibet Plateau from 2015 to 2017. Approximately 69.05 % (232/336) of the diarrhoeic samples were assessed as bovine coronavirus (BCoV)-positive by RT-PCR assay, and most of the detected strains showed a unique evolution based on 40 spike (S), nucleocapsid (N) and haemagglutinin-esterase (HE) gene fragments. Notably, the 12 complete S genes detected shared 1 identical amino acid mutation (E121V) in the S1 subunit compared with the other 150 complete S genes in the GenBank database. Furthermore, a BCoV strain (designated YAK/HY24/CH/2017) was isolated from one diarrhoeic sample (virus titre : 10 8.17 TCID 50 ml À1 ), and a phylogenetic analysis based on complete genome sequences revealed that strain YAK/HY24/CH/2017 has the closest genetic relationship with the BCoV prototype strain Mebus. Interestingly, 2 significant characteristics were observed in the genome of strain YAK/HY24/CH/2017 : (1) the strain had 26 unique amino acid variations in the S gene compared with the other 150 BCoV S genes in the GenBank database and (2) a recombination event was identified between the esterase and lectin domains of the HE gene. In conclusion, this study revealed the high prevalence of BCoV in yaks in the Qinghai-Tibet Plateau. To the best of our knowledge, this is the first description of the molecular prevalence of BCoV in yaks and of a BCoV genome with an HE gene recombination.

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Molecular and phylogenetic analysis of bovine coronavirus based on the spike glycoprotein gene

Cited by 31 publications

References 46 publications

Molecular and antigenic characterization of bovine Coronavirus circulating in Argentinean cattle during 1994–2010

Molecular and antigenic characterization of bovine Coronavirus circulating in Argentinean cattle during 1994–2010

Comparative molecular epidemiology of two closely related coronaviruses, bovine coronavirus (BCoV) and human coronavirus OC43 (HCoV-OC43), reveals a different evolutionary pattern

First detection of bovine coronavirus in Yak (Bos grunniens) and a bovine coronavirus genome with a recombinant HE gene

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