Molecular and antigenic characterization of bovine Coronavirus circulating in Argentinean cattle during 1994–2010

Bok, Marina; Miño, S.; Rodriguez, D.; Badaracco, Alejandra; Nunes, Isabella Paziam Fernandes; Souza, Samilly Evangelista; Bilbao, G.; Uriarte, Enrique Leopoldo Louge; Galarza, R.; Vega, Celina G.; Odeón, Anselmo Carlos; Saif, Linda J.; Parreño, Viviana

doi:10.1016/j.vetmic.2015.10.017

Cited by 32 publications

(57 citation statements)

References 49 publications

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“…This study also aimed to further define BCoV pathogenesis, and to better understand BCoV-specific Ab kinetics. In Argentina, we previously reported a significantly higher rate of BCoV diarrhea in dairy herds compared to beef herds 6 . We detected viral shedding in a small number of animals but high viral circulation manifested through BCoV seroconversion.…”

Section: Discussionmentioning

confidence: 89%

“…As previously suggested, BCoV outbreaks may occur in calves from beef and dairy herds 28,37 . However, in a previous study conducted in Argentina, BCoV infection was mostly associated with diarrhea in dairy husbandry systems 6 . This difference may be due to the close interaction between calves in dairy farms, since these animals were reared under intensive management systems and fed milk replacers lacking Abs, in stark contrast with beef cattle farms, where herds were reared under extensive management systems and calves were fed directly from the dams' milk until they reached 6 months of age 4 .…”

Section: Introductionmentioning

confidence: 86%

“…In Argentina, the BCoV detection rate by ELISA was 1.71% in calves with diarrhea, corresponding to 5.95% of the herds analyzed from 1994 to 2010. Additionally, those Argentina-specific strains were distantly related to the Mebus reference strain in a phylogenetic analysis 6,30 .…”

Section: Introductionmentioning

confidence: 99%

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Passive immunity to control Bovine coronavirus diarrhea in a dairy herd in Argentina

Bok¹,

Alassia²,

Frank³

et al. 2018

Revista Argentina de Microbiología

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Bovine coronavirus (BCoV) is a viral enteric pathogen associated with calf diarrhea worldwide being, in Argentina, mostly detected in dairy husbandry systems. The aim of the present work was to study if maternal IgG1 antibodies (Abs) to BCoV acquired by colostrum intake modulate the development of BCoV infection in calves reared in a dairy farm in Argentina. Thirty Holstein calves were monitored during their first 60 days of age. Animals were classified into two groups depending on their initial BCoV IgG1 Ab titers. The "failure of passive transfer" (FPT) group had significantly lower IgG1 Abs to BCoV than the "acceptable passive transfer" (APT) group of calves (log10 1.98 vs. 3.38 respectively) (p<0.0001). These differences were also observed when the total protein levels in both groups were compared (p=0.0081). Moreover, 71% (5/7) of calves from the FPT group showed IgG1 seroconversion to BCoV compared to 29.4% (5/17) of animals from the APT group. Regarding viral circulation, BCoV was detected in 10% (3/30) of all calves and BCoV IgG1 Ab seroconversion was detected in 42% of the total animals showing that almost half of the calves were infected with BCoV. In conclusion, calves with high titers of specific BCoV IgG1 (≥1024) were mostly protected against viral infection, while animals with low titers of IgG1 (<1024) were mostly infected with BCoV. IgG1 Abs from colostrum origin are critical for prevention of BCoV infection.

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Section: Discussionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 86%

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Passive immunity to control Bovine coronavirus diarrhea in a dairy herd in Argentina

Bok¹,

Alassia²,

Frank³

et al. 2018

Revista Argentina de Microbiología

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“…Screening for bovine RVA was performed using an antigen capture ELISA (Cornaglia et al, 1989) while screening for BCoV was done using an ELISA based on monoclonal antibodies previously described (Smith et al, 1996). These results were reported elsewhere (Badaracco et al, 2012;Bok et al, 2015).…”

Section: Fecal Samplesmentioning

confidence: 99%

Molecular detection of bovine Noroviruses in Argentinean dairy calves: Circulation of a tentative new genotype

Ferragut

Vega

Mauroy

et al. 2016

Infection, Genetics and Evolution

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Bovine noroviruses are enteric pathogens detected in fecal samples of both diarrheic and non-diarrheic calves from several countries worldwide. However, epidemiological information regarding bovine noroviruses is still lacking for many important cattle producing countries from South America. In this study, three bovine norovirus genogroup III sequences were determined by conventional RT-PCR and Sanger sequencing in feces from diarrheic dairy calves from Argentina (B4836, B4848, and B4881, all collected in 2012). Phylogenetic studies based on a partial coding region for the RNA-dependent RNA polymerase (RdRp, 503 nucleotides) of these three samples suggested that two of them (B4836 and B4881) belong to genotype 2 (GIII.2) while the third one (B4848) was more closely related to genotype 1 (GIII.1) strains. By deep sequencing, the capsid region from two of these strains could be determined. This confirmed the circulation of genotype 1 (B4848) together with the presence of another sequence (B4881) sharing its highest genetic relatedness with genotype 1, but sufficiently distant to constitute a new genotype. This latter strain was shown in silico to be a recombinant: phylogenetic divergence was detected between its RNA-dependent RNA polymerase coding sequence (genotype GIII.2) and its capsid protein coding sequence (genotype GIII.1 or a potential norovirus genotype). According to this data, this strain could be the second genotype GIII.2_GIII.1 bovine norovirus recombinant described in literature worldwide. Further analysis suggested that this strain could even be a potential norovirus GIII genotype, tentatively named GIII.4. The data provides important epidemiological and evolutionary information on bovine noroviruses circulating in South America.

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“…as no such study has been conducted previously in neonatal goat kids no literature could be traced for comparative studies. however, BOk et al (2015) detected a high percentage of samples (34.79%) infected with both BCV and GarV in calves. this was somewhat expected, as GarV is responsible for the majority of neonatal calf diarrhea worldwide (kaPIkIaN and ChaNOCk, 1996).…”

Section: Discussionmentioning

confidence: 83%

Molecular detection of Clostridium perfringens toxinotypes, Enteropathogenic Escherichia coli, rotavirus and coronavirus in diarrheic fecal samples of neonatal goat kids

et al. 2018

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In the present study, out of 1156 neonatal goat kids, 238 showing clinical diarrhea were used for detection of toxinotypes of Clostridium perfringens, enteropathogenic E. coli (ePeC), Group a rotavirus (GarV) and Bovine coronavirus (BCV). Isolation and toxinotyping of isolates were done by multiplex Polymerase chain reaction (PCr) using primers for cpa, cpb, cpb2, etx and iap genes. For EPEC, isolation and identification were done using bfpa gene and SYBr green based real time PCr (qPCr). GarV and BCV were detected, by onestep rt-PCr (osrt-PCr). the incidence of C. perfringens was 15.13% with 75% isolates toxinotype a, 25% type D and 61.11% of isolates carrying the β2-toxin gene. The incidence of EPEC was 68.07% based on qPCr, whereas 21.85% were positive for GarV and 15.97% for BCV by osrt-PCr. there was mixed infection of C. perfringens and ePeC in 11.76% and 3.78% for C. perfringens and GarV and 2.1% of C. perfringens and BCV. ePeC and GarV was 19.74% and ePeC plus BCV positivity was 11.34%. GarV and BCV was 5.88%, and 4.20% had mixed infection of ePeC, GarV and BCV. Of the total diarrheic kids sampled, 0.84% had mixed infection of C. perfringens, GARV, BCV and EPEC. On the basis of the above findings, it may be concluded that isolation, multiplex PCr and real time PCr facilitated the characterization of circulating C. perfringens toxinotypes and ePeC in goats reared under semi-arid conditions. the importance of enteritis caused by GarV and BCV and their role in mixed infection in goats requires extensive screening and pathogenicity studies to associate the symptoms with disease.

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Molecular and antigenic characterization of bovine Coronavirus circulating in Argentinean cattle during 1994–2010

Cited by 32 publications

References 49 publications

Passive immunity to control Bovine coronavirus diarrhea in a dairy herd in Argentina

Passive immunity to control Bovine coronavirus diarrhea in a dairy herd in Argentina

Molecular detection of bovine Noroviruses in Argentinean dairy calves: Circulation of a tentative new genotype

Molecular detection of Clostridium perfringens toxinotypes, Enteropathogenic Escherichia coli, rotavirus and coronavirus in diarrheic fecal samples of neonatal goat kids

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