Molecular and Pathological Analysis of Chicken Anemia Virus Isolated from Field Infection in Three Egyptian Provinces

Hussein, Eid; Arafa, Abd El-Satar; Anwar, Nesrin; Khafaga, Asmaa F.

doi:10.14737/journal.aavs/2016/4.5.218.229

Cited by 4 publications

(3 citation statements)

References 31 publications

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“…Many studies reported genetic variations among CAV isolates (Van Santen et al, 2007;Mohamed, 2010;Eltahir, et al, 2011;Hussein et al, 2016) that supports the finding of present study that the detected DNA PCR fragment of 850 bp rather than 675 bp of PCR kit might be attributed to genetic variation that allows the production of different DNA fragment size. Oluwayelu et al (2008) reported genetic differences among Nigerian CAV isolates that grouped them into four clusters.…”

Section: Detection Of the Chicken Anemia Virus Dnasupporting

confidence: 91%

Serological and Molecular Detection of Chicken Anemia Virus in Broiler and Layer Chickens in Iraq.

Al-Ajeeli¹,

Alazawy²,

Ghazuan³

2020

jwpr

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Chicken Anemia Virus (CAV) infects many bird species worldwide and causes immunosuppression. This condition can facilitate the infection of affected birds with other pathogens including bacteria, viruses, and fungi. No data were available on detection or isolation of CAV from birds in Iraq, therefore this study was designed to detect CAV antibodies in broilers and layers in some poultry farms. Accordingly, 200 samples were collected from broiler and layer farms (100 samples each) from different districts of Diyala province and subjected to the ELISA test. Also, 50 tissue samples from embryonated eggs from different hatcheries, four commercial viral vaccines, and 30 ELISA positive samples were subjected to PCR assay to detect the CAV DNA. The results showed that all of broiler and layer farms sampled were serologically positive for CAV antibodies. The overall seropositivity for CAV antibodies for both chicken breeds was 51.5%. In broilers, 43 out of 100 serum samples were positive for CAV antibodies, whereas 60 out of 100 serum samples from layers were CAV antibody-positive. According to age groups, significant differences were observed among one-week-old broilers (30.2%) compared to other age groups. In layers, the age group of 30 weeks showed a seropositivity rate of 33.3%. Conventional PCR test indicated that all tissue samples collected from suspected birds and embryonated eggs were negative for CAV DNA, but only 2 out of 30 serum samples were PCR positive. It is concluded that CAV is endemic in poultry farms of Iraq and may facilitate the vaccination failure against other viruses.

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Section: Detection Of the Chicken Anemia Virus Dnasupporting

confidence: 91%

Serological and Molecular Detection of Chicken Anemia Virus in Broiler and Layer Chickens in Iraq.

Al-Ajeeli¹,

Alazawy²,

Ghazuan³

2020

jwpr

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“…Likewise, Wani et al (2013) also reported higher prevalence (73.3%) of CIAV from clinical samples from 12 different states of India. Higher prevalence of CIAV (51% -93.7%) from the poultry affected with respiratory disease complex was reported from different states of India (Gowthaman et al 2014;Krishan et al 2015;Ganar et al 2016;Hussein et al 2016). This difference in prevalence of CIAV could be due to difference in types of sample collected, geographical distribution of the disease and detection method used.…”

Section: Resultsmentioning

confidence: 95%

“…Haryana ranks 4th in broiler production (0.34 million tonnes per year) in India with an annual growth rate of 12% and per-capita availability of 188 eggs/person/year which is three times higher than the national average (Basic Animal Husbandry Statistics, 2015-2016. A high prevalence (73.3%) of CIA was reported in Haryana (Wani et al 2013).…”

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confidence: 99%

Detection and molecular characterization of chicken infectious anaemia virus in poultry flocks in Haryana

Brar¹,

Bahnot²,

Mahajan³

et al. 2020

Indian J of Anim Sci

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Chicken infectious anaemia virus (CIAV) is an economically important pathogen affecting poultry industry worldwide. Commercial poultry flocks (26) suspected to be affected with chicken infectious anaemia (CIA) were processed for Polymerase Chain Reaction (PCR) using VP2 gene primers and characterized by partial gene sequencing. The PCR revealed 26.9% (7/26) poultry flocks positive for CIAV. Eleven PCR products of CIAV DNA were sequenced. The partial nucleotide sequence analysis of VP2 genes revealed that 11 field strains had 99.2 to 100% similarity among themselves and with the Indian strains. The VP2 gene sequences of 11 field strains showed 97.5 to 100% similarity to the field strains sequences reported from all over the world. On the basis of partial nucleotide sequencing analysis of VP2 gene, our findings suggest that the viral strains circulating in Haryana have similarity to other Indian strains.

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Characterization of full genome sequences of chicken anemia viruses circulating in Egypt reveals distinct genetic diversity and evidence of recombination

Erfan¹,

Selim²,

Naguib

2018

Virus Research

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Molecular and Pathological Analysis of Chicken Anemia Virus Isolated from Field Infection in Three Egyptian Provinces

Cited by 4 publications

References 31 publications

Serological and Molecular Detection of Chicken Anemia Virus in Broiler and Layer Chickens in Iraq.

Serological and Molecular Detection of Chicken Anemia Virus in Broiler and Layer Chickens in Iraq.

Detection and molecular characterization of chicken infectious anaemia virus in poultry flocks in Haryana

Characterization of full genome sequences of chicken anemia viruses circulating in Egypt reveals distinct genetic diversity and evidence of recombination

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