Characterization of full genome sequences of chicken anemia viruses circulating in Egypt reveals distinct genetic diversity and evidence of recombination

Erfan, Ahmed M.; Selim, Abdullah; Naguib, Mahmoud M.

doi:10.1016/j.virusres.2018.05.008

Cited by 24 publications

(17 citation statements)

References 26 publications

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“…Furthermore, strains CIAV/IT/CK/1180/19, CIAV/IT/CK/1155/19 and CIAV/IT/CK/1195/19, clustering with the Del-Ros vaccine strain and having 98.8% mean nucleotide sequence identity in the VP1 gene with the Del-Ros vaccine strain, were interestingly detected in non-vaccinated broilers. Detection of strains with sequences close to the Del-Ros vaccine has been previously reported in Egyptian broilers [34], allowing us to suppose that a vaccine-derived strain or a field strain with a sequence close to this vaccine can circulate. Commercially available CIA vaccines are derived from field strains serially passaged in cells or chicken embryos for attenuation [48].…”

Section: Discussionmentioning

confidence: 75%

Genetic Heterogeneity among Chicken Infectious Anemia Viruses Detected in Italian Fowl

Quaglia

Mescolini

Catelli

et al. 2021

Animals

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Chicken infectious anemia virus (CIAV) is a pathogen of chickens associated with immunosuppression and with a disease named chicken infectious anemia. The present survey reports an epidemiological study on CIAV distribution in Italian broiler, broiler breeder and backyard chicken flocks. Twenty-five strains were detected by a specifically developed nested PCR protocol, and molecularly characterized by partial VP1 gene or complete genome sequencing. Viral DNA amplification was successfully obtained from non-invasive samples such as feathers and environmental dust. Sequence and phylogenetic analysis showed the circulation of field or potentially vaccine-derived strains with heterogeneous sequences clustered into genogroups II, IIIa, and IIIb. Marker genome positions, reported to be correlated with CIAV virulence, were evaluated in field strains. In conclusion, this is the first survey focused on the molecular characteristics of Italian CIAVs, which have proved to be highly heterogeneous, implementing at the same time a distribution map of field viruses worldwide.

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Section: Discussionmentioning

confidence: 75%

Genetic Heterogeneity among Chicken Infectious Anemia Viruses Detected in Italian Fowl

Quaglia

Mescolini

Catelli

et al. 2021

Animals

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“…These hypervariable regions are the most variable region in the VP1 gene of chicken anemia virus as previously mentioned by Islam et al (2002). Phylogenetically, the VP1 gene was divided into many groups in the world that can easily identify the field and vaccines' strains (Eltahir et al, 2011) As previously recorded the CAV circulated in Egypt from different genotypes may be due to multiple introductions (AboElkhair et al, 2014;Abdel-Mawgod et al, 2018;Erfan et al, 2018). In the current study, ten viruses were selected from six different governorates to be sequenced for partial VP1 gene and to be compared with viruses isolated from many countries, such as Germany, India, Japan, Nigeria, Bangladesh, USA, Korea, Taiwan, and China (Schat, 2009).…”

Section: Discussionmentioning

confidence: 88%

“…Chicken anemia virus worldwide is an immunosuppressive disease that circulates in several countries (Natesan et al, 2006;Zhang et al, 2013). In Egypt, the CAV was spread in different governorates causing high mortality and economic losses (Erfan et al, 2018). In the current study, 26 positive samples out of 86 samples were collected from different broiler chicken farms located in six out of 14 governorates in Egypt with a high incidence rate (30%) in Lower Egypt, especially in Sharkia (78%), Ismailia (62.5%), and Alexandria (60%) and most of them were vaccinated.…”

Section: Discussionmentioning

confidence: 99%

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Molecular Characterization of Chicken Anaemia Virus Circulating in Commercial Poultry Flocks in Egypt during 2020

Abdelhalim¹,

Samir²,

Yehia³

2021

WVJ

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Chicken Anemia Virus (CAV) is an extremely contagious immunosuppressive disease causing high economic losses in poultry production. In the present study, tissue samples (bone marrow, thymus, and spleen) were collected from 86 different broiler chicken farms located in fourteen governorates in Egypt during 2020. They suffered from retard growth, weakness, and a drop in egg production with an observed mortality rate ranged 5-15%. A total of 26 samples were positive for CAV using PCR in six governorates in Lower Egypt with a 30% incidence rate, especially in Sharkia (78%), Ismailia (62.5%), and Alexandria (60%). The viral protein1 (VP1) gene of CAV was genetically characterized by sequencing of 10 selected viruses in six governorates. revealing that all Egyptian strains were clustered into two groups (A, B) that was distinct from vaccine strains (Del-Ros, Cux-1, and 26PA) which were clustered in group C. The seven Egyptian viruses in this study (A-Egypt-AN1-2020 to A-Egypt-AN7-2020) were clustered with the viruses from Japan, Argentina, and Malaysia in group A, and the other three viruses (A-Egypt-AN8-2020, A-Egypt-AN9-2020, A-Egypt-AN10-2020) were clustered with the viruses from Nigeria, and India in group B. The Egyptian viruses in the current study acquired new specific mutations clustering them into new subgroups (2A, 2B). By mutation analysis comparing with Del-Rose reference strains, V75I, M97L, and K139Q, E144Q were recorded in all viruses in the group A and B. All Egyptian viruses in the current study had specific new mutations at Y13N, H22N. Moreover, mutation at G74E in Egyptian viruses recorded in the current study was related to sub group 2A, I83V in three strains (A/Egypt/AN1/2020, A/Egypt/AN2/2020, A/Egypt/AN4/2020), and S140A in the hypervariable region was found in four strains (A/Egypt/AN1/2020, A/Egypt/AN2/2020, A/Egypt/AN4/2020 and A/Egypt/AN5/2020) in subgroup 2A. Furthermore, Q139 and Q144 amino acid substitutions, which are important in viral replication, were observed in all viruses. The field viruses in the study were distinct from the vaccinal strains by phylogenetic analysis and A.A. identity. In conclusion, the CAV was continuously circulating in Egypt from different genotypes. It acquired new specific mutations clustering them in a new subgroup, and it was distinct from vaccinal strains. Therefore, it is important to conduct continuous monitoring on the genetic evolution of CAV and further studies on the pathogenicity of the virus and the vaccine efficacy.

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“…Data from whole-genome analysis across public databases reveal that CIAV is classi ed into four clusters, namely: A, B, C, and D [13]. Globally, Clusters A and B are the most common [14].…”

Section: Introductionmentioning

confidence: 99%

Epidemiological Characteristics and Genetic Diversity of Chicken Infectious Anemia Virus (CIAV) in Guangdong Province, China

Zeng¹,

Li²,

Ke³

et al. 2021

Preprint

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Chicken infectious anemia virus (CIAV) is an immunosuppressive disease that causes enormous economic loss to the poultry industry worldwide, transmitted vertically and horizontally. In recent years, CIAV outbreaks have frequently occurred in southern China since 2014, especially in the Guangdong province. However, there is a lack of epidemiological studies of CIAV in Guangdong province. We performed PCR by using the serum and tissue samples collected from seven cities of Guangdong province. The PCR-positive tissue samples were inoculated into SPF eggs and MSB1 cells for virus isolation by blind-passages three times. This study isolated and characterized 325 serum samples and four tissue samples, the total positive CIAV rate of 62.15% in serum samples, and all four tissues were positive for the CIAV. However, the virus could only be isolated from MSB1 cells. Sequence analysis revealed that the four isolated comparisons with those in the GenBank showed high nucleotide similarities and were near clade C2. Our data showed over 60% of positive cases for CIAV serological analysis consist of seven cities, Which may lead to severe immunosuppressive effects in chickens. In summary, these findings provide new insights to guide molecular epidemiological surveillance of CIAV in Guangdong province.

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Characterization of full genome sequences of chicken anemia viruses circulating in Egypt reveals distinct genetic diversity and evidence of recombination

Cited by 24 publications

References 26 publications

Genetic Heterogeneity among Chicken Infectious Anemia Viruses Detected in Italian Fowl

Genetic Heterogeneity among Chicken Infectious Anemia Viruses Detected in Italian Fowl

Molecular Characterization of Chicken Anaemia Virus Circulating in Commercial Poultry Flocks in Egypt during 2020

Epidemiological Characteristics and Genetic Diversity of Chicken Infectious Anemia Virus (CIAV) in Guangdong Province, China

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