Molecular and Morphological Diversity of Fusarium Species in Finland and North-Western Russia

Yli-Mattila, Tapani; Paavanen-Huhtala, Sari; Parikka, Päivi; Konstantinova, Pavlina; Gagkaeva, T. Yu.

doi:10.1023/b:ejpp.0000032397.65710.69

Cited by 82 publications

(41 citation statements)

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“…Phylogenetic analysis of F. avenaceum strains from different regions of Russia using the PHO gene sequences showed four of them to form an independent cluster phylogenetically closer to F. torulosum, than to F. avenaceum, which is conventionally called F. avenaceum-V. Perhaps this group of strains can be treated as a separate phylogenetic species, but this fact has not been confirmed in the analysis of other markers and requires further study. At the same time, we have not found a correlation between the phylogeny of the strains and their division into conventional groups I and II proposed by T. Yli-Mattila et al [31,38]. Moreover, these markers did not allow to identify F. arthrosporioides strain as a separate taxa other than F. avenaceum, thereby confirming the hypothesis that F. arthrosporioides is not a separate species.…”

supporting

confidence: 62%

MOLECULAR GENETIC APPROACHES FOR INVESTIGATION OF TAXONOMY AND SPECIFIC IDENTIFICATION OF TOXIN-PRODUCING Fusarium SPECIES: ACHIEVEMENTS AND PROBLEMS (review)

Stakheev¹,

Samokhvalova²,

Ryazantsev³

et al. 2016

S-h. biol.

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A b s t r a c tAscomycetous fungi of the genus Fusarium, which was separated as a single taxonomic group in 1809 by Heinrich Friedrich Link, are distributed worldwide and have different ecological niches. They have been found in soil and plants as endophytes, saprotrophs and parasites. Infections of agricultural plants, caused by Fusarium fungi, lead to annual damage, estimated in hundred million dollars. In addition to decrease harvest quality and quantity, Fusarium fungi are able to produce toxic metabolites (fusariotoxins). The consumption of fusariotoxin-contaminated winter grain led to deaths of tens of thousands of people in Volga and Ural regions in 1930s-1940s. These factors make necessary investigation of morphology, biochemistry and genetics of those organisms. Nowadays there is no universal taxonomic system of the genus Fusarium, so it is not possible to identify an isolate to species level in many cases. The high variability of morphological structures, and, on the other side, their similarity for closely related species, are major problems for researchers which use classical methods for identification and systematization of this group of fungi. Today, the molecular methods, based on the use of specific DNA sequence, are playing an increasingly important role in Fusarium systematics. The application of this approach has led to establishment of a number of new species in the genus. T. Yli-Mattila et al. (2009, 2011 used multilocus phylogenetic analysis to describe the novel species F. sibiricum and F. ussurianum which were found in Siberia and Far East and proved to be morphologically similar to F. graminearum and F. langsethiae, respectively. The authors of this paper identified the species F. torulosum (morphologically similar to F. avenaceum) in Russia for the first time based on the use of DNA markers. It is no less important that the analysis of interand intraspecific polymorphism makes possible the development of highly-specific assays for molecular detection of Fusarium fungi, including ones which infect plants and produce mycotoxins. The use of these assays (for example, ones described by M. Nicolaisen et al., 2009 and A.A. Stakheev et al., 2011) allows not only to detect the contamination of plants by mycotoxin producers, but also to estimate the quantity of their content in a probe. At the same time, a lot of questions are still unanswered: particularly, simultaneous application of different species concepts (morphological, biological and phylogenetic) makes it difficult to develop universal taxonomic system of the genus; also the most informative and reliable marker, which would reflect the evolutionary history of the genus, has not been found. The main achievements and problems of Fusarium phylogenetics, and perspectives of the application of molecular approach for studies of this group of organisms are discussed in this review.

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supporting

confidence: 62%

MOLECULAR GENETIC APPROACHES FOR INVESTIGATION OF TAXONOMY AND SPECIFIC IDENTIFICATION OF TOXIN-PRODUCING Fusarium SPECIES: ACHIEVEMENTS AND PROBLEMS (review)

Stakheev¹,

Samokhvalova²,

Ryazantsev³

et al. 2016

S-h. biol.

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“…The DNA quality of each strain was confirmed by using ITS1/ITS4 primers [26]. The species-specific primer pair PfusF/FlanR for F. langsethiae [4] was used in PCR reactions. The primers CNL12/PulvIGS [3] were used to distinguish two F. langsethiae IGS-subgroups based on the PCR product sizes.…”

Section: Dna Isolation and Pcr Amplificationmentioning

confidence: 99%

“…Later, molecular methods used in combination with traditional morphological methods revealed that F. langsethiae resembles F. poae, F. sporotrichioides and F. sibiricum phylogenetically [2][3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Identification and Characterization of Spontaneous Auxotrophic Mutants in <em>Fusarium langsethiae</em>

Gavrilova¹,

Skritnika²,

Gagkaeva³

2017

Preprint

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Analysis of 49 strains of F. langsethiae originating from northern Europe (Russia, Finland, Sweden, UK, Norway, and Latvia) revealed the presence of spontaneous auxotrophic mutants that reflect natural intraspecific diversity. Our investigations detected that 49.0% of F. langsethiae strains were auxotrophic mutants for biotin, and 8.2% of the strains required thiamine as a growth factor. They failed to grow on vitamin-free media. For both prototrophic and auxotrophic strains, no growth defect was observed in rich organic media. Without essential vitamins, a significant reduction in the growth of the auxotrophic strains results in a decrease of the formation of T-2 toxin and diacetoxyscirpenol. In addition, all analysed F. langsethiae strains were distinguished into two subgroups based on PCR product sizes. According to our results, 26 and 23 strains of F. langsethiae belong to subgroups I and II respectively. We determined that the deletion in the IGS region of the rDNA of F. langsethiae belonging to subgroup II is linked with temperature sensitivity and causes a decrease in strain growth at 30 °C. Four thiamine auxotrophic strains were found in subgroup I, while 21 biotin auxotrophic strains were detected in subgroups II. To the best of our knowledge, the spontaneous mutations in F. langsethiae observed in the present work have not been previously reported.

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“…However, Fusarium diversity after Pichia treatment was not analyzed in this study, and therefore we cannot conclude which speciWc species were inhibited during malting. In Finland, the most common Fusarium species in barley during the recent years have been F. avenaceum, F. athrosporioides, F. sporotrichioides, and F. culmorum [72]. Our further studies will be directed toward investigating the eVect of biocontrol yeasts on Fusarium diversity during processing.…”

Section: B)mentioning

confidence: 99%

Yeasts isolated from industrial maltings can suppress Fusarium growth and formation of gushing factors

Laitila

Sarlin

Kotaviita

et al. 2007

J Ind Microbiol Biotechnol

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Fusarium infection of barley and malt can cause severe problems in the malting and brewing industry. In addition to being potential mycotoxin producers, Fusarium fungi are known to cause beer gushing (spontaneous overfoaming of beer). Cereal-derived bacteria and yeasts are potential biocontrol agents. In this study, the antifungal potential of selected yeasts (12 strains) derived from the industrial malting ecosystem was studied in vitro with a plate-screening assay. Several ascomycetous yeast strains showed antagonistic activity against field and storage moulds, Pichia anomala being the most effective strain. The effects of P. anomala VTT C-04565 (C565) were examined in laboratory scale malting with naturally contaminated barley exhibiting gushing potential. P. anomala C565 restricted Fusarium growth and hydrophobin production during malting and prevented beer gushing. Grain germination was not disturbed by the presence of yeast. Addition of P. anomala C565 into the steeping seemed to retard wort filtration, but the filtration performance was recovered when yeast culture was combined with Lactobacillus plantarum VTT E-78076. Well-characterized microbial cultures could be used as food-grade biocontrol agents and they offer a natural tool for tailoring of malt properties.

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Molecular and Morphological Diversity of Fusarium Species in Finland and North-Western Russia

Cited by 82 publications

References 0 publications

MOLECULAR GENETIC APPROACHES FOR INVESTIGATION OF TAXONOMY AND SPECIFIC IDENTIFICATION OF TOXIN-PRODUCING Fusarium SPECIES: ACHIEVEMENTS AND PROBLEMS (review)

MOLECULAR GENETIC APPROACHES FOR INVESTIGATION OF TAXONOMY AND SPECIFIC IDENTIFICATION OF TOXIN-PRODUCING Fusarium SPECIES: ACHIEVEMENTS AND PROBLEMS (review)

Identification and Characterization of Spontaneous Auxotrophic Mutants in <em>Fusarium langsethiae</em>

Yeasts isolated from industrial maltings can suppress Fusarium growth and formation of gushing factors

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