Molecular and biochemical investigations of key antioxidant/oxidant molecules in Saudi patients with recurrent miscarriage

Al‐Sheikh, Yazeed A.; Ghneim, Hazem K.; Alharbi, Adel F.; Alshebly, Mashael M.; Aljaser, Feda; Aboul‐Soud, Mourad A. M.

doi:10.3892/etm.2019.8082

Cited by 19 publications

(29 citation statements)

References 61 publications

(88 reference statements)

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“…A recent study reported significant increase of SOA in plasma samples of RPL patients compared to the control samples (45.2 ± 6.10 nmol/ml vs 35.3 ± 5.45 nmol/ml). Similar difference was observed for the placental tissue SOA between the two groups (5.93 ± 0.78 µmol/min/mg in RPL patients and 4.67 ± 0.62 µmol/min/mg in healthy pregnant women) [ 8 ].…”

Section: Main Textsupporting

confidence: 79%

“…Reported plasma MDA levels were 5.95 ± 0.91 pM for recurrent miscarriage patients and 5.12 ± 0.81 pM for healthy controls [ 9 ]. Similarly in another study, placental MDA levels were highly significantly increased in recurrent miscarriage subjects compared to the controls (334 ± 45.8 nmol/g wet weight versus 258 ± 35.7 nmol/g wet weight) [ 8 ].…”

Section: Main Textmentioning

confidence: 75%

“…Ghneim et al, reported increased levels of plasma H 2 O 2 in RPL group compared to healthy women and non-pregnant women (7.4 ± 0.70 pM; 6.70 ± 0.70 pM; 6.20 ± 0.70 pM; respectively) [ 9 ]. Comparably, Al-Sheikh and coauthors found increased levels of this marker in placental tissues of recurrent miscarriage patients 3.38 ± 0.46 nmol/min/mg in RM group and 2.41 ± 0.35 nmol/min/mg in healthy pregnant subjects [ 8 ].…”

Section: Main Textmentioning

confidence: 99%

“…Importantly, increased levels of SOA and H 2 O 2 in both studies were associated with depletion of enzymatic antioxidants such as superoxide dismutase (SOD) catalase (CAT), glutathione reductase (GR) and glutathione peroxidase (GPx) as well as decreased expression of all examined antioxidant genes (GPx, GR, SOD and CAT) [ 8 , 9 ].…”

Section: Main Textmentioning

confidence: 99%

“…Recent studies highlight the role of oxidative stress and oxidative biomarkers in the pathophysiology of recurrent pregnancy loss [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

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The role of oxidative stress in patients with recurrent pregnancy loss: a review

Zejnullahu

Kosumi

2021

Reprod Health

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Background Recurrent pregnancy loss (RPL) presents one of the main problems in the field of reproductive medicine, due to the unknown etiology in 50% of cases, as well as limited evidence-based diagnostic and therapeutic modalities. Recent studies indicate that systemic and placental oxidative stress (OS) represents an essential factor in the etiopathogenesis of RPL. This article is a comprehensive narrative synthesis of previously published studies concerning the role of oxidative stress in the etiology of recurrent pregnancy loss. Methods We conducted literature search of published studies in the English language focusing on oxidative stress and its association with recurrent pregnancy loss (RPL) utilizing the Medline and Cochrane databases from 2000 through January 2021. The keywords used were “recurrent pregnancy loss” “oxidative stress and recurrent pregnancy loss” and “oxidative stress biomarkers and recurrent pregnancy loss”. Results The search yielded 1116 publications, of which 92 were included in the final analysis. Reactive oxygen species (ROS) and reactive nitrogen species (RNS) at basal levels have various physiological functions whereas deviation from redox window is associated with different pathologies including early pregnancy loss. The currently available studies support the concept that increased oxidative stress (OS) and deficient antioxidant protection is implicated in the etiology of recurrent pregnancy loss (RPL) but underlying mechanisms through which OS affects pregnancy outcome remains largely indefinable. Conclusions Future research in this field can provide new insights regarding the OS-mediated damage in recurrent pregnancy loss as well as potential applications of antioxidant therapy in this group of patients.

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Section: Main Textsupporting

confidence: 79%

Section: Main Textmentioning

confidence: 75%

Section: Main Textmentioning

confidence: 99%

Section: Main Textmentioning

confidence: 99%

“…Recent studies highlight the role of oxidative stress and oxidative biomarkers in the pathophysiology of recurrent pregnancy loss [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

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The role of oxidative stress in patients with recurrent pregnancy loss: a review

Zejnullahu

Kosumi

2021

Reprod Health

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Effects of unpredictable chronic mild stress on the cellular redox state and mitochondrial energy homeostasis in rat adipose tissue: A comprehensive metabolic study

Abulmeaty,

Ghneim,

Almajwal

et al. 2023

Cell Biochemistry & Function

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Unpredictable chronic mild stress (UCMS) leads to variable metabolic effects. Oxidative stress (OS) of adipose tissue (AT) and mitochondrial energy homeostasis is little investigated. This work studied the effects of UCMS on OS and the antioxidant/redox status in AT and mitochondrial energy homeostasis in rats. Twenty‐four male Wistar rats (180–220 g) were divided into two equal groups; the normal control (NC) group and the UCMS group which were exposed to various stresses for 28 days. An indirect calorimetry machine was used to measure volumes of respiratory gases (VO2& VCO2), total energy expenditure (TEE), and food intake (FI). The AT depots were collected, weighed, and used for measuring activities and gene expression of key antioxidant enzymes (GPx1, SOD, CAT, GR, GCL, and GS), OS marker levels including superoxide anion (SA), peroxynitrite radical (PON), nitric oxide (NO), hydrogen peroxide (H2O2), lipid peroxides (LPO), t‐protein carbonyl content (PCC), and reduced/oxidized glutathione levels (GSH, GSSG). Additionally, AT mitochondrial fractions were used to determine the activities of the tricarboxylic acid cycle (TCA) cycle enzymes (CS, α‐KGDH, ICDH, SDH, MDH), respiratory chain complexes I–III, II–III, IV, the nicotinamide coenzymes NAD+, NADH, and ATP/ADP levels. Compared with the NC group, the UCMS group showed very significantly increased OS marker levels, lowered antioxidant enzyme activities and gene expression, as well as lowered TCA cycle and respiratory chain activity and NAD+, NADH, and ATP levels (p < .001 for all comparisons). Besides, the UCMS group had lowered TEE and insignificant FI and weight gain. In conclusion, AT of the UCMS‐subjected rats showed a state of disturbed redox balance linked to disrupted energy homeostasis producing augmentation of AT.

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Omega‐3 fatty acid supplements and recurrent miscarriage: A perspective on potential mechanisms and clinical evidence

Huo

Wang

et al. 2023

Food Science & Nutrition

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Recurrent miscarriage (RM) affects approximately 1%–5% of couples worldwide. Due to its complicated etiologies, the treatments for RM also vary greatly, including surgery for anatomic factors such as septate uterus and uterine adhesions, thyroid modulation drugs for hyperthyroidism and hypothyroidism, and aspirin and low molecular weight heparin for antiphospholipid syndrome. However, these treatment modalities are still insufficient to solve RM. Omega‐3 fatty acids are reported to modulate the dysregulation of immune cells, oxidative stress, endocrine disorders, inflammation, etc., which are closely associated with the pathogenesis of RM. However, there is a lack of a systematic description of the involvement of omega‐3 fatty acids in treating RM, and the underlying mechanisms are also not clear. In this review, we sought to determine the potential mechanisms that are highly associated with the pathogenesis of RM and the regulation of omega‐3 fatty acids on these mechanisms. In addition, we also highlighted the direct and indirect clinical evidence of omega‐3 fatty acid supplements to treat RM, which might encourage the application of omega‐3 fatty acids to treat RM, thus improving pregnancy outcomes.

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Molecular and biochemical investigations of key antioxidant/oxidant molecules in Saudi patients with recurrent miscarriage

Cited by 19 publications

References 61 publications

The role of oxidative stress in patients with recurrent pregnancy loss: a review

The role of oxidative stress in patients with recurrent pregnancy loss: a review

Effects of unpredictable chronic mild stress on the cellular redox state and mitochondrial energy homeostasis in rat adipose tissue: A comprehensive metabolic study

Omega‐3 fatty acid supplements and recurrent miscarriage: A perspective on potential mechanisms and clinical evidence

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