Modulatory effects of EPA and DHA on proliferation and apoptosis of pancreatic cancer cells

Abstract

In order to investigate the effects of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on the proliferation, apoptosis of pancreatic cancer cell line SW1990 cells and the expression of cyclin E mRNA, the SW1990 cells were treated with different concentrations of EPA or DHA (20, 40, 60 microg/mL) for 0, 12, 24, 36 and 48 h respectively. By using MTT method, the inhibitory effects of EPA or DHA on the cell growth were assayed. Real time PCR was used to detect the expression changes of cyclin E mRNA after the SW1990 cells were treated with 40 microg/mL EPA or DHA for different time. Flow cytometry was used to test the changes of apoptostic rate in the SW1990 cells treated with different concentrations of EPA or DHA for 24 h. The results showed that EPA and DHA could inhibit the growth of SW1990 cells in a time-and concentration-dependent manner (P<0.01). EPA or DHA could also significantly inhibit the expression of cyclin E mRNA in a time-dependent manner (P<0.05). EPA or DHA could induce the apoptosis of SW1990 cells in a concentration-dependent manner (P<0.01). It was concluded that omega-3 fatty acid could inhibit the proliferation of pancreatic cancer cell line SW1990 cells and promote their apoptosis. The down-regulation of the cyclin E expression by omega-3 fatty acid might be one of the mechanisms for its anti-tumor effect on pancreatic cancer.

Omega-3-Polyunsaturated Fatty Acids Suppress Pancreatic Cancer Cell Growth in vitro and in vivo via Downregulation of Wnt/Beta-Catenin Signaling

“…ω3-PUFAs potently inhibited cell proliferation and induced apoptosis, as indicated by the increased sub-G 1 population, caspase-3 activity and PARP cleavage in human pancreatic cancer cells. These data are in line with studies showing that ω3-PUFAs induce apoptosis [31,32,33,34] in human pancreatic cancer cells.…”

Effect of ω-3 polyunsaturated fatty acid on toll-like receptors in patients with severe multiple trauma

“…Suppression of the excessive inflammatory reaction and improvement of the immune function are critical for the early stage of severe trauma healing process. ω-3PUFA, comprised of linoleic acid, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), is one of the essential fatty acids for human beings, and the two components of ω-3PUFA, EPA and DHA, were found in some studies to provide immune nutrition and inhibit proliferation of cancer cells [3][4][5] . Previous studies showed that ω-3PUFA…”

Optimization of the Lipase Catalyzed Production of Structured Acylglycerols With Polyunsaturated Fatty Acids Isolated From Sardine Oil

“…DHA has proven capable to reduce the risk of heart disease and inflammatory cytokines (Simopoulos, 2002;Von Schacky, 2007). Additionally, DHA inhibits tumour cells growth (Zhang, Long, Zhang, & Wang, 2007) and it can be consumed as acylglycerol for its moderate absorption and less oxidation compared to the free fatty acid form (Valenzuela, Valenzuela, Sanhueza, & Nieto, 2005). Different methods have been developed to obtain n-3 PUFA extracts (as acylglicerides or free fatty acids) from marine oils; some of those methods have been combined to increase both efficiency and yield of the extraction (Gamez, Noriega, Medina, Ortega, García, & Angulo, 2003).…”