Modulatory Activity of Bee Pollen Against the Toxicity of Antituberculosis Drugs Rifampicin and Isoniazid in Testis of Sprague Dawley Rats

Abstract

Objective: The aim of this study is assessment of protective role of bee pollen in antituberculosis drug (rifampicin and isoniazid)-induced toxicity in testis of Sprague Dawley rats.Methods: Healthy rats weighing 180±20 g were selected for the study. Rats were divided into five groups, i.e., Group A (control), Group B (100 mg/kg body weight/day rifampicin-treated), Group C (rifampicin 100 mg/kg body weight and bee pollen 100 mg/kg body weight), Group D (isoniazid 50 mg/kg body/day treated), and Group E (isoniazid 50 mg/kg body weight/day with bee pollen 100 mg/kg body weight/day) serve as experimental groups.Results: Aqueous extract of bee pollen when administered along with the antituberculosis drugs (rifampicin, isoniazid) showed significant reduction in the level of malondialdehyde while the activity of superoxide dismutase, glutathione (GSH) reductase, glutathione peroxidase, glutathioneStransferase, catalase, and GSH was elevated representing the antioxidant potential of bee pollen against the drug-treated groups. Supplementation of bee pollen significantly reduced histological changes in the testis of drug-induced groups such as smaller epithelial height, germ cell loss, and irregular seminiferous tubules to near normal. Conclusion:Bee pollen has shown the modulatory effect against damage and oxidative stress induced by antituberculosis drugs (rifampicin and isoniazid) in rat testis.

Antioxidant and hepatorenal protective effects of bee pollen fractions against propionic acid‐induced autistic feature in rats

“…Reduced levels of antioxidants such as GSH, GST, and CAT were reported in rat testis after oral administration of tuberculosis (TB) drugs. In their study, a significant increase in GSH, GST, and CAT activities was reported after BP administration along with TB drugs (Bharti, Kumar, & Kaur, 2017). Another study conducted by Shayakhmetova, Bondarenko, and Kovalenko (2012) reported that rats administered with TB drugs showed increased levels of epithelium exfoliation into seminiferous tubules (Shayakhmetova et al, 2012).…”

Effect of bee pollen extract on lead-induced toxicity in rat testis

“…Extract of Phyllanthus emblica has a protective effect against lead acetate (Saha et Verma, 2015). In bee pollen treated group, the thickness of germinal epithelium was also improved and recovery of germ cell population, in Sprague Dawley rats treated with antituberculotic drugs (Bharti et al, 2017). So far, Bee pollen and date palm pollen showed an ameliorative effect on rat testis of diabetic rats.…”

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“…Bharti et al, 23 supplemented the diet of rats with bee pollen which showed antioxidant and protective effect when given along with antituberculosis drugs (rifampicin as well as isoniazid). It is thus observed that bee pollen has strong potential to provide protection against antituberculosis drugs-induced toxicity in rats as evidenced by improved histological parameters and rebuilding of the antioxidant/ oxidative status.…”