Effect of bee pollen extract on lead-induced toxicity in rat testis

Bouazza, Sofiane; Demmouche, Abbassia; Toumi-Benali, Fawzia; Zouba, M; Bahri, M R; Agher, N.; Merakchi, Nacera; Ahmar, Mama El

doi:10.38150/sajeb.8(3).p91-102

Cited by 4 publications

(1 citation statement)

References 53 publications

(65 reference statements)

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“…3,4 Many studies revealed that lead acetate could cause infertility in male. 5 A study revealed that giving 0.1% lead acetate to rats had reduced testis weight, seminiferous tubulous diameter, epididymal sperm count, and serum testosterone level. 6 study revealed that 20 mg/kg BW lead acetate could caused the decrease of testis weight and diameter of tubulous seminiferous in male mice testis.…”

Section: Introductionmentioning

confidence: 99%

Protective Effect of Lemongrass Extract ( Cymbopogon nardus) on Spermatozoa Cells Induced by Lead Acetate

Setiyowati

Solekha

Irawan

et al. 2022

BHSJ

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Introduction: Male infertility can be caused by many factors. One of which is environmental pollution such as lead acetate. Lead acetate exposure can increase Reactive Oxygen Species (ROS), affecting spermatozoa quality. Natural antioxidants and flavonoid on Lemongrass (C. nardus) have a great potential for protecting the male reproductive system. This study aimed to analyze the effect of giving various dosages of C. nardus extract on the sperm quality of mice. Methods: The Balb/C male mice were divided into five equal groups. The negative control group was injected with 0.01 ml Na-CMC 0.5% within 40 days, and the positive control group was injected with 0.01 ml lead acetate 7 mg/kg BW within 5 days, the treatment groups were injected by 0.01 ml lead acetate 7 mg/kg body weight within 5 days and continuously injected by 0.01 ml of C. nardus extract with various dosage 25 mg/kg BW, 50 mg/kg BW, and 100 mg/kg BW within 35 days. At the end of the experiment, mice were sacrificed, and sperm suspensions were collected from cauda epididymal to measure the morphology, concentration, and motility.Results: The results showed that giving C. nardus extract could repair morphology, concentration, and motility of spermatozoa with significantly different (p<0.05). The 25 mg/kg BW dose has a good protective effect. Conclusion: The C. nardus extract can repair the decreasing sperm quality caused by lead acetate exposure.

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Section: Introductionmentioning

confidence: 99%

Protective Effect of Lemongrass Extract ( Cymbopogon nardus) on Spermatozoa Cells Induced by Lead Acetate

Setiyowati

Solekha

Irawan

et al. 2022

BHSJ

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Protective effect of bee pollen in acute kidney injury, proteinuria, and crystalluria induced by ethylene glycol ingestion in rats

Elghouizi

Al-Waili

Elmenyiy

et al. 2022

Sci Rep

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Oxidative stress plays a role in hyperoxaluria-induced kidney injury and crystallization. Bee pollen is a hive product with a high content of antioxidants. The antioxidant content and protective effect of bee pollen extract (BPE) against ethylene glycol (EG) induced crystalluria, and acute kidney injury (AKI) were investigated. The effect of BPE on the EG-induced liver injury and proteinuria was also examined. Ten groups of male Wister rats were treated daily with vehicle, cystone, BPE (100, 250, and 500 mg/kg b.wt.), and group 6–9 treated with EG, EG + BPE (100, 250, and 500 mg/kg b.wt.) and group 10 EG + cystone. The dose of EG was 0.75% v/v, and the dose of cystone was 500 mg/kg b.wt. On day 30, blood and urine samples were collected for analysis. Kidneys were removed for histopathological study. The antioxidant activity of BPE was assessed, and its total phenols and flavonoids were determined. EG significantly increased urine parameters (pH, volume, calcium, phosphorus, uric acid, and protein), blood urea, creatinine, and liver enzymes (P < 0.05). EG decreased creatinine clearance and urine magnesium and caused crystalluria. Treatment with BPE or cystone mitigates EG's effect; BPE was more potent than cystone (P < 0.05). BPE increases urine volume, sodium, and magnesium compared to the control and EG treated groups. BPE reduces proteinuria and prevents AKI, crystalluria, liver injury, and histopathological changes in the kidney tissue caused by EG. BPE might have a protective effect against EG-induced AKI, crystalluria, proteinuria, and stone deposition, most likely by its antioxidant content and activity.

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