Protective effect of bee pollen in acute kidney injury, proteinuria, and crystalluria induced by ethylene glycol ingestion in rats

Abstract

Oxidative stress plays a role in hyperoxaluria-induced kidney injury and crystallization. Bee pollen is a hive product with a high content of antioxidants. The antioxidant content and protective effect of bee pollen extract (BPE) against ethylene glycol (EG) induced crystalluria, and acute kidney injury (AKI) were investigated. The effect of BPE on the EG-induced liver injury and proteinuria was also examined. Ten groups of male Wister rats were treated daily with vehicle, cystone, BPE (100, 250, and 500 mg/kg b.wt.), and group 6–9 treated with EG, EG + BPE (100, 250, and 500 mg/kg b.wt.) and group 10 EG + cystone. The dose of EG was 0.75% v/v, and the dose of cystone was 500 mg/kg b.wt. On day 30, blood and urine samples were collected for analysis. Kidneys were removed for histopathological study. The antioxidant activity of BPE was assessed, and its total phenols and flavonoids were determined. EG significantly increased urine parameters (pH, volume, calcium, phosphorus, uric acid, and protein), blood urea, creatinine, and liver enzymes (P < 0.05). EG decreased creatinine clearance and urine magnesium and caused crystalluria. Treatment with BPE or cystone mitigates EG's effect; BPE was more potent than cystone (P < 0.05). BPE increases urine volume, sodium, and magnesium compared to the control and EG treated groups. BPE reduces proteinuria and prevents AKI, crystalluria, liver injury, and histopathological changes in the kidney tissue caused by EG. BPE might have a protective effect against EG-induced AKI, crystalluria, proteinuria, and stone deposition, most likely by its antioxidant content and activity.

Ameliorative Effects of Propolis and Royal Jelly against CCl4‐Induced Hepatotoxicity and Nephrotoxicity in Wistar Rats

“…Histopathological studies of the liver and kidneys showed an improvement in cellular architecture after propolis therapy and confirmed its hepatoprotective efficacy [46] . Bee pollen reduces proteinuria and prevents ethylene glycol‐induced acute kidney injury, crystalluria, liver injury, and histopathological changes in kidney tissue [47] …”

“…[46] Bee pollen reduces proteinuria and prevents ethylene glycol-induced acute kidney injury, crystalluria, liver injury, and histopathological changes in kidney tissue. [47]…”

Translational Research on Bee Pollen as a Source of Nutrients: A Scoping Review from Bench to Real World

“…In addition, studies of BP extracts obtained by diverse solvents, including ethanolic [ 148 , 156 , 167 , 168 ], hydroethanolic [ 15 , 154 , 171 , 179 ], and diverse organic solvent-mediated extracts [ 150 , 151 , 153 , 175 ], have noted important antioxidant activities regardless of the extraction media. These bioeffects were also widely reported in animal models by studies that focused on different anatomical organs and physiological systems including renal [ 174 ], nervous [ 139 , 174 ], digestive [ 144 , 151 ], and reproductive [ 172 , 185 ] systems, as well as on different biochemical, hematological, and toxicological markers at systemic levels [ 137 , 165 , 184 ].…”

The Ameliorating Effect of Artificial Coffee from Mangrove Fruits (Rhizophora mucronata) on T Lymphocyte Cells and Renal Histopathology of BALB/c Mice Induced by Lipopolysaccharide

“…When related to the compound class of mangrove fruit artificial coffee detected by GC-MS analysis, previous studies have reported antioxidant compounds of the fatty aldehyde class can provide protective effects against oxidative stress-related damage in the renal (Zhang et al, 2021). The phenolic aldehyde-like class of compounds from bee pollen extract was reported to exert protective effects against histological changes in the renal, including crystal deposition, collecting tubule dilatation, tubular epithelial necrosis, inflammation, edema, and interstitium congestion (Elghouizi et al, 2022). The fatty acid class of compounds was also reported to attenuate toxicity in the renal by modulating oxidative stress and cell apoptosis (Elsafty et al, 2023).…”