Modulation of sperm tail protein tyrosine phosphorylation by pentoxifylline and its correlation with hyperactivated motility

Nassar, Ahmed; Mahony, M.C.; Morshedi, Mahmood; Lin, Mingwei; Srisombut, Chartchai; Oehninger, Sergio

doi:10.1016/s0015-0282(99)00013-8

Cited by 110 publications

(84 citation statements)

References 21 publications

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“…Cyclic AMP, in turn, is believed to stimulate the cAMP-dependent kinase (24), which itself induces sperm tail protein phosphorylation (25) with subsequent increase in sperm motility (26). Improving acrosomal reaction is another benefi cial effect of pentoxifylline drug, demonstrated that this induction is not due to a Ca 2+ -infl ux in the sperm cell but is according to increasing the intracellular level of cAMP as the second messenger of acrosomal reaction (27,28).…”

Section: Discussionmentioning

confidence: 99%

Chorionic morphine, naltrexone and pentoxifylline effect on hypophyso-gonadal hormones of male rats

Moradi¹,

Mahmoodi²,

Raoofi³

et al. 2015

BLL

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Abstract:Background: Knowledge about harmful effects of morphine on hormone secretion seems to be necessary. The aim of the present study was to evaluate the effect of pentoxifylline on side effects derived by morphine on hypophyso-gonadal hormones of male rats. Methods: 32 male rats were divided into the 4 groups of OSS: control (received 40 g Sucrose/l drinking water and intraperitoneal injection of 1 l/kg normal saline), OMS: morphine group (received 0.4 mg/l + 40 g Sucrose/l in drinking water and intraperitoneal injection of 1 l/kg normal saline), NMS: morphine+naltrexane group (received 0.4 mg/l + 40 g Sucrose/l in drinking water and IP injection dose of 10 mg/kg/ml/day Naltrexane) and PMS: morphine + pentoxifylline group (received 0.4 mg/dl + 40 g Sucrose/l in drinking water and IP injection dose of 12 mg/kg/ml/day Pentoxifylline) for 56 days, respectively. Results: Serum levels of testosterone, LH, FSH hormones were measured. Pentoxifylline increased serum levels of testosterone, LH, FSH hormones compared to control, morphine and morphine-naltrexane groups. Conclusion: Pentoxifylline has a signifi cant effi cacy for increasing serum levels of sexual hormones. Considering that Pentoxifylline is safe and cheap, with easy application, we suggest for the usage of this drug for improving semen parameter's quality before performing ART for the treatment of morphine addicts (Fig. 1, Ref. 31). Text in PDF www.elis.sk.

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Section: Discussionmentioning

confidence: 99%

Chorionic morphine, naltrexone and pentoxifylline effect on hypophyso-gonadal hormones of male rats

Moradi¹,

Mahmoodi²,

Raoofi³

et al. 2015

BLL

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“…In most species studied, sperm tyrosine-phosphorylated proteins are mainly located at the flagellum during the course of capacitation and fertilization (Carrera et al 1996, Leclerc et al 1997, Mahony & Gwathmey 1999, Si & Okuno 1999, Lewis & Aitken 2001, Urner et al 2001, Petrunkina et al 2003, Pommer et al 2003, and the presence of tyrosine phosphorylation in the principal piece has been correlated with the acquisition of hyperactivated motility (Nassar et al 1999, Si & Okuno 1999, Petrunkina et al 2003. In addition, a capacitation-associated redistribution of phosphotyrosine residues to the acrosome has been reported in boars (Petrunkina et al 2001, Tardif et al 2001, bulls (Cormier & Bailey 2003) and buffalo (Roy & Atreja 2008), and specific phosphotyrosine proteins localized over the acrosomal region have been postulated to be involved in the zona pellucida interaction and/or fusion events (Leyton & Saling 1989, Naz et al 1991, Ficarro et al 2003, Dube et al 2005.…”

Section: Introductionmentioning

confidence: 99%

Changes in content and localization of proteins phosphorylated at tyrosine, serine and threonine residues during ram sperm capacitation and acrosome reaction

Grasa

Colás²,

Gallego³

et al. 2009

Reproduction

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Previously, we reported the involvement of tyrosine phosphorylation in events that lead to ram sperm capacitation. In this study, we carried out a comparative analysis of the localization of tyrosine, serine and threonine phosphoproteins in different functional stages of ram spermatozoa (after the swim-up procedure, in vitro capacitation, and ionophore-induced acrosome reaction) by immunofluorescence, immunocytochemistry and confocal microscopy. Capacitation increased protein tyrosine, serine and threonine phosphorylation whereas the induction of the acrosome reaction resulted in significantly decreased phosphorylation, mainly in those proteins that increased following capacitation. Control samples showed tyrosine-phosphorylated proteins restricted to the head, mainly distributed at the equatorial region with some cells also displaying an acrosomal and/or post-acrosomal localization. In vitro capacitation promoted both tail and acrosome phosphorylation, and the acrosome reaction induced the loss of labeling on the acrosome and the subsequent increase in the post-acrosomal region and flagellum. The preferential localization of serine-and threonine-phosphorylated proteins in the equatorial and acrosomal regions found in control samples changed during capacitation, which induced tail phosphorylation in a sequential manner. After the acrosome reaction, the labeling of both phosphoamino acids decreased in the acrosome and increased in the post-acrosome. The obtained results were proved by two immunodetection techniques and strengthened by confocal microscopy, and indicate that changes in phosphorylated proteins during capacitation and acrosome reaction of ram spermatozoa may have physiological significance in consolidating certain phosphorylated proteins to specific sperm regions involved in acrosomal exocytosis and zona pellucida recognition, binding and penetration. Reproduction (2009) 137 655-667

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“…PTX has been considered to stimulate flagellar motility by increasing sperm intracellular cAMP (Stefanovich, 1973;Garbers & Kopf, 1980;Tash & Means, 1983;Ward & Clissold, 1987) as well as by reducing sperm intracellular superoxide anion and reactive oxygen species known to damage DNA (Lopes et al, 1998;Twigg et al, 1998). In particular PTX in both in vivo and in vitro studies, appears to increase significantly beat cross frequency, curvilinear velocity, and the percentage of hyperactivated spermatozoa (Rees et al, 1990;Shen et al, 1991;Lewis et al, 1993;Moohan et al, 1993;Pang et al, 1993;Tournaye et al, 1994;Centola et al, 1995;Paul et al, 1996;Nassar et al, 1999).…”

Section: The Development Of In Vivo Studiesmentioning

confidence: 99%

The Role of PDE5 Inhibitors in the Treatment of Testicular Dysfunction

Dimitriadis¹,

Baltogiannis²,

Koukos³

et al. 2012

Male Infertility

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Modulation of sperm tail protein tyrosine phosphorylation by pentoxifylline and its correlation with hyperactivated motility

Cited by 110 publications

References 21 publications

Chorionic morphine, naltrexone and pentoxifylline effect on hypophyso-gonadal hormones of male rats

Chorionic morphine, naltrexone and pentoxifylline effect on hypophyso-gonadal hormones of male rats

Changes in content and localization of proteins phosphorylated at tyrosine, serine and threonine residues during ram sperm capacitation and acrosome reaction

The Role of PDE5 Inhibitors in the Treatment of Testicular Dysfunction

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