Chorionic morphine, naltrexone and pentoxifylline effect on hypophyso-gonadal hormones of male rats

Abstract

Abstract:Background: Knowledge about harmful effects of morphine on hormone secretion seems to be necessary. The aim of the present study was to evaluate the effect of pentoxifylline on side effects derived by morphine on hypophyso-gonadal hormones of male rats. Methods: 32 male rats were divided into the 4 groups of OSS: control (received 40 g Sucrose/l drinking water and intraperitoneal injection of 1 l/kg normal saline), OMS: morphine group (received 0.4 mg/l + 40 g Sucrose/l in drinking water and intraperitoneal injection of 1 l/kg normal saline), NMS: morphine+naltrexane group (received 0.4 mg/l + 40 g Sucrose/l in drinking water and IP injection dose of 10 mg/kg/ml/day Naltrexane) and PMS: morphine + pentoxifylline group (received 0.4 mg/dl + 40 g Sucrose/l in drinking water and IP injection dose of 12 mg/kg/ml/day Pentoxifylline) for 56 days, respectively. Results: Serum levels of testosterone, LH, FSH hormones were measured. Pentoxifylline increased serum levels of testosterone, LH, FSH hormones compared to control, morphine and morphine-naltrexane groups. Conclusion: Pentoxifylline has a signifi cant effi cacy for increasing serum levels of sexual hormones. Considering that Pentoxifylline is safe and cheap, with easy application, we suggest for the usage of this drug for improving semen parameter's quality before performing ART for the treatment of morphine addicts (Fig. 1, Ref. 31). Text in PDF www.elis.sk.

Tribulus terrestris Hydroalcoholic Extract Administration Effects on Reproductive Parameters and Serum Level of Glucose in Diabetic Male Rats

“…The serum samples were then kept in deep freezer (-18 ºC) until measurement of testosterone hormone by ELISA method. Also, the testes were separated and weighed separately and then preserved in neutral buffered formalin 10 % (Keshtmand et al, 2015;Moradi et al, 2015).…”

Therapeutic Effect of Resveratrol on Morphine-Induced Damage in Male Reproductive System of Mice by Reducing Nitric Oxide Serum Level

“…Many studies have revealed the infertility effects of morphine administration on males by decreasing testosterone serum level and inducing histological damage on testis and sperms (Yilmaz et al, 1999;Moradi et al, 2014). Studies have shown the existence of morphine receptors in sertoli and leydig cells of testis and have further addressed the involvement of inflammation and oxidation during male reproductive system exposure to morphine by producing NO and regulating inflammatory cascades (Jalili et al, 2016;Chehrei et al, 2017).…”

Pentoxifylline besides naltrexone recovers morphine-induced inflammation in male reproductive system of rats by regulating Toll-like receptor pathway

“…on fertility by decreasing serum level of pituitary-gonad axis as we previously have represented in male rats (Bliesener et al, 2005;Cicero, Davis, LaRegina, Meyer, & Schlegel, 2002;Moradi et al, 2015;Sokolowska-Mikolajczyk, Socha, & Mikolajczyk, 2005). α: Statistical difference between control (OS)and experimental groups (p < 0.001).…”

“…Regarding male sex hormone secretions, it have been shown that prescription of morphine reduces plasma levels of testosterone and LH in rats (Moradi, Mahmoodi, Raoofi, & Ghanbari, 2015;Yilmaz et al, 1999) and the activation of kisspeptin-GPR54 system promoting hypothalamus-pituitary-gonadal (HPG) axis offered to explain the reduction in testosterone level of morphine-treated subjects (Mahmoudi, Khazali, & Janahmadi, 2014). The dissimilarities of histological features, seen following morphine utilisation, have been described by free radical production leading to oxidative stress in tissues such as testis (Liu et al, 2014) and kidney (Patel et al, 2003).…”

“…However, we previously observed that the effect of pentoxifylline (PTX) drug, a phosphodiesterase inhibitor, on hypophysial-gonadal axis hormones (LH, testosterone) of male morphine-treated rats was higher than opioid antagonist (naltrexone; Moradi et al, 2015). Moreover, studies established the anti-inflammatory effects of pentoxifylline through inhibition of pro-inflammatory cytokine (interleukin-1-beta, interleukin-6 and other mediators) secretion and reduction in superoxide anion production by increasing intracellular levels of cAMP (cyclic adenosine monophosphate) that demonstrates the efficacy of the drug on male reproductive system according to anti-inflammatory characteristics of the drug (Aliabadi, Karimi, Rasti, Akmali, & Esmaeilpour, 2013;Dorazil-Dudzik et al, 2004;Esteves, Spaine, & Cedenho, 2007;Liu et al, 2007;Mika, Wawrzczak-Bargiela, Osikowicz, Makuch, & Przewlocka, 2009;Moslemi Mehni, Ketabchi, & Hosseini, 2014;Tardif et al, 2014;Wei et al, 2009).…”