The Role of PDE5 Inhibitors in the Treatment of Testicular Dysfunction

Dimitriadis, Fotios; Baltogiannis, D.; Koukos, Sotirios; Giannakis, Dimitrios; Tsounapi, Panagiota; Seminis, G.; Saito, Motoaki; Takenaka, Atsushi; Sofikitis, Nikolaos

doi:10.5772/32501

Cited by 1 publication

(2 citation statements)

References 145 publications

(172 reference statements)

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“…Repair of erectile dysfunction is done by detecting the PDE5 gene [12], then giving treatment with chemical compounds that believe to act as PDE5 inhibitors [13]. Erectile dysfunction is the inability to achieve or maintain penile erection sufficient to have sexual intercourse with a partner [14].…”

Section: Introductionmentioning

confidence: 99%

“…The erection process normally depends on the balance process between the contraction phase and the relaxation of the smooth muscle cells of the penis that is influenced by the production of cyclic guanosine monophosphate (cGMP) [13]. Cyclic guanosine monophosphate is not continuously present because it will subsequently be degraded by PDE5 [12].…”

Section: Introductionmentioning

confidence: 99%

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The Analysis of Primer Gene of Phosphodiesterase Type 5 (PDE5) on Erectyle Dysfungction

Watuguly¹,

Samsuria²,

Astuti³

et al. 2018

OJAppS

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Backround: Erectile dysfunction (ED) is the inability of male reproductive organs within sexual intercourse caused by neurogenic and hormonal disorders. There are some causes of ED such as hypertension, stress, neurological disorders, stroke, diabetes, atherosclerosis, lifestyle, alcohol, smoking, and age-related hormonal decline can cause infertility. The natural treatment of sexual dysfunction through aphrodisiac activity of the plant is to increase sexual hormones, spermatogenesis activity and through PDE5 inhibitors such as sildenafil, verdenafil, and tadalafil which can inhibit the hydrolysis of second messenger cGMP of penis smooth muscle cells. Primer Design for amplification of several PDE5 gene nucleotide sequences obtained from NCBI gen banks and tested directly through explosions at NCBI and also using MEGA 6, primer 3 plus, and fast PCR software. The natural treatment of sexual dysfunction through aphrodisiac activity from plants to increase sexual hormone, spermatogenesis activity and inhibitor PDE5 such as sildenafil, vardenafil, and tadalafil can inhibit hydrolysis second messenger of cells cGMP smooth penis muscles. Method: Primer design stages for several sequences are data supply, multiple sequence alignment, sequence trimming, primer design (fastPCR input), in silico PCR analysis, and primer evaluation (Primer Test, OligoCalc and BLAST). Results: Primer of PDE5 that is choosen is with reverse sequence 5-TGCATTGACCATGTCTCTCGTT-3, forward 5-CGCCGATCTGGGCTGAACTA-3 able to amplify template DNA at temperature 67.2˚C, 65.8˚C, 63.7˚C, however, the DNA band fragment looks not very clear, while it is more clearly seen at Tm temperature 61.2˚C, 59.1˚C, 57.8˚C and 57˚C. Conclusion:

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%