Modulation of inflammation and oxidative stress in canine chondrocytes

Dycus, David L.; Au, A.Y.; Grzanna, M.W.; Wardlaw, Jennifer L.; Frondoza, Carmelita G.

doi:10.2460/ajvr.74.7.983

Cited by 12 publications

(11 citation statements)

References 57 publications

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“…However, higher than normal intracellular ROS concentrations can still overpower the homeostatic proteins and cause oxidative damage to the cell and contribute to the onset and progression of OA by inducing chondrocyte death and matrix degradation [65]. It has been shown that MDA, as a marker of lipid peroxidation, is increased in inflamed cartilage tissue and synovial fluid [23, 28, 66] as well as in blood of humans [29, 67] and dogs [32] suffering from OA. In vitro assays have demonstrated cartilage degradation mediated by lipid peroxidation [8].…”

Section: Discussionmentioning

confidence: 99%

“…L-carnitine protects cells against oxidative damage since it acts as a free radical scavenger and has an important function in lipid metabolism by transporting fatty acids across the inner mitochondrial membrane [79]. Abundant evidence indicates the importance of GSH in maintaining the antioxidant homeostasis in cartilage and during articular pathological conditions [23, 27, 29, 67, 80–83]. Glutathione is a tripeptide (y-Glutamyl-cysteinyl-glycine) thiol present in virtually all animal cells – in normal conditions, both the reduced (GSH) and oxidized (GSSG) forms of glutathione remain in a balanced state [53].…”

Section: Discussionmentioning

confidence: 99%

“…Reactive oxygen species (ROS), associated with pro-inflammatory cytokines and prostaglandins, have been shown to play a deleterious role in the course of joint diseases, leading to cartilage damage [20, 21] and progressive chronic inflammation [22, 23]. Comparable changes in the synovia immune cellularity have been described in dogs as those occurring in humans [24, 25].…”

Section: Introductionmentioning

confidence: 99%

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Evaluating oxidative stress, serological- and haematological status of dogs suffering from osteoarthritis, after supplementing their diet with fish or corn oil

et al. 2016

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BackgroundOxidative stress plays an important role in the pathogenesis of disease, and the antioxidant physiological effect of omega-3 from fish oil may lead to improvement of canine spontaneous osteoarthritis (OA).MethodsIn this prospective randomized, controlled, double-blinded study, we assessed haematological and biochemical parameters in dogs with OA following supplementation with either a concentrated omega-3 deep sea fish oil product or corn oil. Blood samples from 77 client-owned dogs diagnosed as having OA were taken before (baseline) and 16 weeks after having orally ingested 0.2 ml/Kg bodyweight/day of deep sea fish oil or corn oil. Circulating malondialdehyde (MDA), glutathione (GSH), non-transferrin bound iron (NTBI), free carnitine (Free-Car), 8-hydroxy-2-deoxyguanosine (8-OH-dG), and serum fatty acids, haemograms and serum biochemistry were evaluated. Differences within and between groups from baseline to end, were analysed using repeated samples T-test or Wilcoxon rank test and independent samples T-test or a Mann-Whitney test.ResultsSupplementation with fish oil resulted in a significant reduction from day 0 to day 112 in MDA (from 3.41 ± 1.34 to 2.43 ± 0.92 μmol/L; P < 0.001) and an elevation in Free-Car (from 18.18 ± 9.78 to 21.19 ± 9.58 μmol/L; P = 0.004) concentrations, whereas dogs receiving corn oil presented a reduction in MDA (from 3.41 ± 1.34 to 2.41 ± 1.01 μmol/L; P = 0.001) and NTBI (from −1.25 ± 2.17 to −2.31 ± 1.64 μmol/L; P = 0.002). Both groups showed increased (albeit not significantly) GSH and 8-OH-dG blood values. Dogs supplemented with fish oil had a significant reduction in the proportions of monocytes (from 3.84 ± 2.50 to 1.77 ± 1.92 %; P = 0.030) and basophils (from 1.47 ± 1.22 to 0.62 ± 0.62 %; P = 0.012), whereas a significant reduction in platelets counts (from 316.13 ± 93.83 to 288.41 ± 101.68 × 109/L; P = 0.029), and an elevation in glucose (from 5.18 ± 0.37 to 5.32 ± 0.47 mmol/L; P = 0.041) and cholesterol (from 7.13 ± 1.62 to 7.73 ± 2.03 mmol/L; P = 0.011) measurements were observed in dogs receiving corn oil.ConclusionsIn canine OA, supplementation with deep sea fish oil improved diverse markers of oxidative status in the dogs studied. As corn oil also contributed to the reduction in certain oxidative markers, albeit to a lesser degree, there was no clear difference between the two oil groups. No clinical, haematological or biochemical evidence of side effects emerged related to supplementation of either oil. Although a shift in blood fatty acid values was apparent due to the type of nutraceutical product given to the dogs, corn oil seems not to be a good placebo.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluating oxidative stress, serological- and haematological status of dogs suffering from osteoarthritis, after supplementing their diet with fish or corn oil

et al. 2016

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“…Furthermore, it regulates transcription as a co-activator [ 37 , 38 ]. The chondrocyte apoptosis induced by reactive oxygen species (ROS) has been considered important in OA pathogenesis [ 39 , 40 ]. Our results have demonstrated that DJ-1 is up-regulated in the early stages of OA, which suggested its involvement in the regeneration of cartilage due to the prevention chondrocyte death via the removal of ROS.…”

Section: Resultsmentioning

confidence: 99%

Proteomic analysis of rat cartilage: the identification of differentially expressed proteins in the early stages of osteoarthritis

2014

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BackgroundOsteoarthritis (OA) is a chronic degenerative disease of the articular cartilage, and its diagnosis is based on symptoms and radiological signs that are only present in the late stages of the disease. Due to the limitations in diagnosing OA before the onset of symptoms, such as pain, little is known about the molecular mechanisms involved in the pathogenesis of OA. Experimental OA models are often used to study the kinetics of the progression of this disease. In this report, we conducted a proteomic study of osteoarthritic cartilage during the early stages of OA using an experimental rat model.ResultsTen proteins that are differentially expressed under early OA conditions were identified by 2-DE and MALDI-TOF/MS. These proteins mediated many processes, such as glycolysis and energy production (Nme2 and Pnp), cartilage matrix (Col2a1), transcription and protein synthesis (Eef1a1 and DJ-1), signal transduction (CaM and Pebp1), transport (Alb and Hba1), and latexin (Lxn). In addition, changes in Lxn expression in early OA were observed and validated by western blot and immunofluorescence analysis.ConclusionsThe proteins that we identified indicate that energy metabolism, cartilage matrix remodelling, and protective cellular mechanisms are associated with early OA. In addition, latexin expression during the early stages of OA could be implicated in cartilage repair.Electronic supplementary materialThe online version of this article (doi:10.1186/s12953-014-0055-0) contains supplementary material, which is available to authorized users.

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“…Moreover, it has been applied in diabetes [23], cardiovascular disease [24] and liver-kidney disease [25]. ALA can eliminate ROS and reduce the production of ROS by targeting NADPH oxidase [26][27][28][29]. Therefore, compared with general antioxidants, ALA can not only more comprehensively eliminate active substances in vivo but also target NADPH oxidase.…”

Section: Introductionmentioning

confidence: 99%

Alpha-Lipoic Acid Prevents Atrial Electrical and Structural Remodeling via Inhibition of NADPH Oxidase in a Rabbit Rapid Atrial Pacing Model

Chen

Zhang

et al. 2020

Preprint

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Background The pathogenesis of atrial fibrillation(AF) is complex, and the treatment method is still not satisfactory. A rapid atrial pacing (RAP) model was constructed to study the effects of alpha-lipoic acid (ALA) on electrical and structural remodeling, as well as its possible mechanism in rabbits.Methods A total of 30 rabbits were randomly divided into a sham-operated group (SHAM group), a rapid atrial pacing model group (RAP group) and an alpha-lipoic acid+rapid atrial pacing model group (ALA+RAP group). Their right atriums were paced at a speed of 600 beats/min for 12 h in the RAP and ALA+RAP groups, and the atrial effective refractory period (AERP) and AERP frequency adaptability were determined during the pace. In each group, malondialdehyde (MDA), superoxide dismutase (SOD) and reactive oxygen species (ROS) were detected to observe the effects of oxidative stress. The pathological structure of the atrial tissue was observed through HE and Masson staining. Ultrastructural changes in the atrial myocytes were observed by transmission electron microscopy (TEM), and the expression levels of Nox2 and Nox4 were detected by immunohistochemistry, western blot and ELISA.Conclusion ALA can inhibit atrial electrical remodeling and structural remodeling by reducing ROS production and alleviating oxidative stress injury induced by rapid right atrial pacing, and its mechanism may be related to inhibiting the activity of NADPH oxidase.

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Modulation of inflammation and oxidative stress in canine chondrocytes

Abstract: iii ACKNOWLEDGEMENTS

Cited by 12 publications

References 57 publications

Evaluating oxidative stress, serological- and haematological status of dogs suffering from osteoarthritis, after supplementing their diet with fish or corn oil

Evaluating oxidative stress, serological- and haematological status of dogs suffering from osteoarthritis, after supplementing their diet with fish or corn oil

Proteomic analysis of rat cartilage: the identification of differentially expressed proteins in the early stages of osteoarthritis

Alpha-Lipoic Acid Prevents Atrial Electrical and Structural Remodeling via Inhibition of NADPH Oxidase in a Rabbit Rapid Atrial Pacing Model

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