Proteomic analysis of rat cartilage: the identification of differentially expressed proteins in the early stages of osteoarthritis

Parra-Torres, Nancy Marbella; Cázares-Raga, Febe Elena; Kourí, Juan B.

doi:10.1186/s12953-014-0055-0

Cited by 6 publications

(4 citation statements)

References 42 publications

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“…The cells were lysed using ice-cold RIPA buffer containing protease inhibitor (Boster Biological Technology, China). Proteins from cartilage samples were extracted as previous reports [16,17]. Briefly, the frozen tissues were mechanically pulverized, sonicated, and stored in cold extraction buffer (Roche) at 4°C overnight.…”

Section: Western Blottingmentioning

confidence: 99%

Icariin alleviates osteoarthritis by inhibiting NLRP3-mediated pyroptosis

Zu¹,

Mu²,

Li³

et al. 2019

J Orthop Surg Res

123

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Background Osteoarthritis (OA) is the common chronic degenerative joint bone disease that is mainly featured by joint stiffness and cartilage degradation. Icariin (ICA), an extract from Epimedium, has been preliminarily proven to show anti-osteoporotic and anti-inflammatory effects in OA. However, the underlying mechanisms of ICA on chondrocytes need to be elucidated. Methods LPS-treated chondrocytes and monosodium iodoacetate (MIA)-treated Wistar rats were used as models of OA in vitro and in vivo, respectively. LDH and MTT assays were performed to detect cytotoxicity and cell viability. The expression levels of NLRP3, IL-1β, IL-18, MMP-1, MMP-13, and collagen II were detected by qRT-PCR and Western blotting. The release levels of IL-1β and IL-18 were detected by ELISA assay. Caspase-1 activity was assessed by flow cytometry. Immunofluorescence and immunohistochemistry were used to examine the level of NLRP3 in chondrocytes and rat cartilage, respectively. The progression of OA was monitored with hematoxylin-eosin (H&E) staining and safranin O/fast green staining. Results ICA could suppress LPS-induced inflammation and reduction of collagen formation in chondrocytes. Furthermore, ICA could inhibit NLRP3 inflammasome-mediated caspase-1 signaling pathway to alleviate pyroptosis induced by LPS. Overexpression of NLRP3 reversed the above changes caused by ICA. It was further confirmed in the rat OA model that ICA alleviated OA by inhibiting NLRP3-mediated pyroptosis. Conclusion ICA inhibited OA via repressing NLRP3/caspase-1 signaling-mediated pyroptosis in models of OA in vitro and in vivo, suggesting that ICA might be a promising compound in the treatment of OA.

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Section: Western Blottingmentioning

confidence: 99%

Icariin alleviates osteoarthritis by inhibiting NLRP3-mediated pyroptosis

Zu¹,

Mu²,

Li³

et al. 2019

J Orthop Surg Res

123

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“…just normal vs early RA) without any validation. The genes which were selected for the models are not unknown: LXN is known to be upregulated in early OA [53] and not only as part of the inflammatory response but also influencing the perception of pain [54]; several CXCL genes (chemokines) have been shown to be upregulated at RA, also CXCL8 [11, 12]; MAB21L2 is upregulated especially in OA [55]; about the RP11-* genes less is known as these labels are still their original clone ID [56].…”

Section: Resultsmentioning

confidence: 99%

Analysis of gene expression in rheumatoid arthritis and related conditions offers insights into sex-bias, gene biotypes and co-expression patterns

et al. 2019

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The era of next-generation sequencing has mounted the foundation of many gene expression studies. In rheumatoid arthritis research, this has led to the discovery of important candidate genes which offered novel insights into mechanisms and their possible roles in the cure of the disease. In the last years, data generation has outstripped data analysis and while many studies focused on specific aspects of the disease, a global picture of the disease is not yet accomplished. Here, we analyzed and compared a collection of gene expression information from healthy individuals and from patients suffering under different arthritis conditions from published studies containing the following clinical conditions: early and established rheumatoid arthritis, osteoarthritis and arthralgia. We show comprehensive overviews of this data collection and give new insights specifically on gene expression in the early stage, into sex-dependent gene expression, and we describe general differences in expression of different biotypes of genes. Many genes that are related to cytoskeleton changes (actin filament related genes) are differently expressed in early rheumatoid arthritis in comparison to healthy subjects; interestingly, eight of these genes reverse their expression ratio significantly between men and women compared early rheumatoid arthritis and healthy subjects. There are some slighter changes between men and woman between the conditions early and established rheumatoid arthritis. Another aspect are miRNAs and other gene biotypes which are not only promising candidates for diagnoses but also change their expression grossly in average at rheumatoid arthritis and arthralgia compared to the healthy condition. With a selection of intersecting genes, we were able to generate simple classification models to distinguish between healthy and rheumatoid arthritis as well as between early rheumatoid arthritis to other arthritides based on gene expression.

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“…Recently, numerous studies have found that abnormal expression of LXN can cause inflammatory diseases in vivo, such as colitis and acute pancreatitis [ 24 , 25 ]. In OA-related studies, LXN had been shown to be highly expressed at the early stage of OA and was associated with articular cartilage mineralization [ 26 , 27 ]. Importantly, LXN a downstream regulator of the circ_0094742/miR-127-5p axis, has been reported to mediate OA development by regulating chondrocyte viability [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

Circ_0002715 promotes the development of osteoarthritis through regulating LXN by sponging miR-127-5p

et al. 2023

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Background Our study aims to investigate the role and mechanism of circular RNA_0002715 (circ_0002715) in osteoarthritis (OA) progression. Methods IL-1β-induced CHON-001 cells were used to mimic OA cell model. Circ_0002715, microRNA (miR)-127-5p and Latexin (LXN) expression was detected by quantitative real-time PCR. Cell functions were determined by MTT assay, flow cytometry and ELISA assay. Protein expression was examined by western blot. Results Circ_0002715 was highly expressed in OA cartilage tissues. Circ_0002715 silencing inhibited inflammation, apoptosis, and ECM degradation in IL-1β-interfered CHON-001 cells. Circ_0002715 could sponge miR-127-5p, and miR-127-5p could target LXN. The effect of circ_0002715 down-regulation on chondrocyte injury was partially restored by miR-127-5p inhibitor. MiR-127-5p could suppress chondrocyte injury by inhibiting LXN expression. Conclusion Circ_0002715 might be a new therapeutic target for OA, which regulated miR-127-5p/LXN axis to promote IL-1β-induced chondrocyte injury.

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Proteomic analysis of rat cartilage: the identification of differentially expressed proteins in the early stages of osteoarthritis

Cited by 6 publications

References 42 publications

Icariin alleviates osteoarthritis by inhibiting NLRP3-mediated pyroptosis

Icariin alleviates osteoarthritis by inhibiting NLRP3-mediated pyroptosis

Analysis of gene expression in rheumatoid arthritis and related conditions offers insights into sex-bias, gene biotypes and co-expression patterns

Circ_0002715 promotes the development of osteoarthritis through regulating LXN by sponging miR-127-5p

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