Modulating effect of losartan potassium on the mutagenicity and recombinogenicity of doxorubicin in somatic cells of Drosophila melanogaster

Silva-Oliveira, R.G.; Orsolin, Priscila Capelari; Nepomuceno, Júlio César

doi:10.1016/j.fct.2016.07.003

Cited by 8 publications

(6 citation statements)

References 45 publications

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“…Comparison of the frequencies of wing spots in the MH flies (mwh/flr 3 genotype) and BH flies (mwh/TM3 genotype) from both ST an HB crosses indicated that induced recombination was the major response for the treatments with DXR alone. These findings were further supported by previous investigations with DXR in Drosophila wing SMART (Valadares et al, 2008;De Rezende et al, 2009;Orsolin et al, 2016;Silva-Oliveira et al, 2016;Oliveira et al, 2017).…”

Section: Discussionsupporting

confidence: 88%

Modulating effect of vitamin D3 on the mutagenicity and carcinogenicity of doxorubicin in Drosophila melanogaster and in silico studies

Vasconcelos

Orsolin

Oliveira

et al. 2020

Food and Chemical Toxicology

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Vitamin D3 (VD3) deficiency increases DNA damage, while supplementation may exert a pro-oxidant activity, prevent viral infections and formation of tumors. The aim of this study was to investigate the mutagenicity and carcinogenicity of VD3 alone or in combination with doxorubicin (DXR) using the Somatic Mutation and Recombination Test and the Epithelial Tumor Test, both in Drosophila melanogaster. For better understanding of the molecular interactions of VD3 and receptors, in silico analysis were performed with molecular docking associated with molecular dynamics. Findings revealed that VD3 alone did not increase the frequency of mutant spots, but reduced the frequency of mutant spots when co-administered with DXR. In addition, VD3 did not alter the recombinogenic effect of DXR in both ST and HB crosses. VD3 alone did not increase the total frequency of tumor, but significantly reduced the total frequency of tumor when co-administered with DXR. Molecular modeling and molecular dynamics between calcitriol and Ecdysone Receptor (EcR) showed a stable interaction, indicating the possibility of signal transduction between VD3 and EcR. In conclusion, under these experimental conditions, VD3 has modulatory effects on the mutagenicity and carcinogenicity induced by DXR in somatic cells of D. melanogaster and exhibited satisfactory interactions with the EcR.

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Section: Discussionsupporting

confidence: 88%

Modulating effect of vitamin D3 on the mutagenicity and carcinogenicity of doxorubicin in Drosophila melanogaster and in silico studies

Vasconcelos

Orsolin

Oliveira

et al. 2020

Food and Chemical Toxicology

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“…80 LOS and all its TPs presented alerts for carcinogenicity, with the exception of LOS TP1. Despite this, previous studies found that LOS has no genotoxic effect, 61 indicating that it does not induce cancer and has antineoplastic effects. 81 The IBP TPs (IBP TP4 and IBP TP7) only presented a single positive alert for carcinogenicity, while IBP TP13 and IBP TP19 showed between 2 and 4 positive alerts.…”

Section: Carcinogenicitymentioning

confidence: 88%

“…Although the in silico results for some of the models showed mutagenicity alerts for LOS and its TPs, previous in vivo and in vitro studies had reported an absence of genotoxic effects for LOS. 61,62 Studies carried out using rats have evaluated the possible mutagenic effects of NMS, finding that this drug is potentially genotoxic. 63,64 Here, the in silico results showed that NMS and its TPs presented SAs for mutagenicity, with the aromatic nitro SA being the most prevalent.…”

Section: Environmental Science: Water Research and Technology Papermentioning

confidence: 99%

In silico environmental risk assessment of fate and effects of pharmaceuticals and their TPs generated and treated by coupling tertiary processes in hospital wastewater

Della-Flora

Scunderlick

Wilde

et al. 2023

Environ. Sci.: Water Res. Technol.

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“…DXR was used in the present study as a positive control at 0.4 mM (diluted in autoclaved reverse osmosis water) for in vivo tests. DXR at 0.4 mM was previously able to generate ROS and induce homologous recombination in Drosophila melanogaster through topoisomerase inhibition [ 21 , 47 , 48 , 49 ].…”

Section: Methodsmentioning

confidence: 99%

Copper(II) Complex Containing 4-Fluorophenoxyacetic Acid Hydrazide and 1,10-Phenanthroline: A Prostate Cancer Cell-Selective and Low-Toxic Copper(II) Compound

et al. 2022

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Prostate Cancer (PCa) is the second leading cause of cancer-related deaths among men worldwide. The treatment of advanced cases is based on chemotherapy, which lacks specificity and efficacy, due to severe side effects and resistance to the traditional drugs. Copper complexes have shown antitumoral efficacy and low toxicity, being considered a promising class of metal-based drugs for the treatment of malignant neoplasms. Thus, the present study aimed to evaluate the cellular effects of a copper(II) complex with 4-fluorophenoxyacetic acid hydrazide and 1,10-phenanthroline (1) on PCa cell lines, as well as the mutagenic/recombinogenic and anticarcinogenic potential of 1 in Drosophila melanogaster. PNT-2 (non-tumorigenic), LNCaP (hormone-responsive PCa) and PC-3 (androgen-independent PCa) cells were cultured, and cytotoxicity was assessed using the MTT assay. The expression levels of the proliferation markers Ki-67 and Cyclin D1 were analyzed by flow cytometry. Furthermore, the Somatic Mutation and Recombination Test (SMART) and the Epithelial Tumor Test (ETT) were performed. Complex 1 was selective to LNCaP cells, significantly reducing Ki-67 and Cyclin D1 expression levels. Sub-toxic concentrations of complex 1 were defined by the toxicity test in D. melanogaster, and no mutagenic/recombinogenic/carcinogenic effects were observed. Anticarcinogenic potential was observed in D. melanogaster, suggesting modulating activity of the complex 1 against Doxorubicin, a drug used as control by its carcinogenic properties. Therefore, complex 1 is a possible starting point for the development of new antitumor agents for the treatment of PCa.

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Modulating effect of losartan potassium on the mutagenicity and recombinogenicity of doxorubicin in somatic cells of Drosophila melanogaster

Cited by 8 publications

References 45 publications

Modulating effect of vitamin D3 on the mutagenicity and carcinogenicity of doxorubicin in Drosophila melanogaster and in silico studies

Modulating effect of vitamin D3 on the mutagenicity and carcinogenicity of doxorubicin in Drosophila melanogaster and in silico studies

In silico environmental risk assessment of fate and effects of pharmaceuticals and their TPs generated and treated by coupling tertiary processes in hospital wastewater

Copper(II) Complex Containing 4-Fluorophenoxyacetic Acid Hydrazide and 1,10-Phenanthroline: A Prostate Cancer Cell-Selective and Low-Toxic Copper(II) Compound

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