Modulating effect ofBiophytum sensitivumextract on rats with acetic acid-induced ulcerative colitis

Abstract

Context: Traditionally, Biophytum sensitivum (L.) DC (Oxalidaceae) is used in Indian medicine to treat diseases include stomachache, convulsions, cramps, inflammation, and ulcer. Objective: The present study examines the effect of aerial parts of B. sensitivum (methanol extract) on a murine model of ulcerative colitis (UC). Materials and methods: UC was induced by intracolonic injection of 3% acetic acid in Wistar rats. B. sensitivum (50 or 100 mg/kg b wt) or reference drug sulfasalazine (100 mg/kg b wt) was administrated intra-peritoneally for 5 consecutive days before induction of colitis. Results: In the present study, we demonstrated for the first time that the administration of B. sensitivum (50 mg/kg b wt) was found to inhibit colitis by lowering macroscopic score (up to 3.66 ± 0.77) and also showed significant reduction (p50.01) in lactate dehydrogenase (LDH) and myeloperoxidase (MPO) activities. Furthermore, a significant reduction (p50.01) in mucosal content of lipid peroxidation (LPO), glutathione (GSH), superoxide dismutase (SOD), and nitric oxide (NO) confirms that B. sensitivum could significantly inhibit colitis. The study showed significant reduction (p50.01) in colonic tumor necrosis factor-a (TNF-a), interleukin-1-b (IL-1b), and IL-6 levels as well as the expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) after treatment compared with the colitis control group. The histopathological study also confirms the foregoing findings. Treatment with B. sensitivum was also able to inhibit the activation and translocation of transcription factors, nuclear factor (NF)-kB subunits (p65/p50). Conclusion: These results suggest that B. sensitivum exhibits protective effect against acetic acid-induced UC.

Histological Study on the Effect of Adipose Mesenchymal Stem Cells Derived Microvesicles and the Role of its RNA Content on Experimentally-Induced Ulcerative Colitis in Albino Rats

“…Morphometric measurements documented a statistically significant rise in the mean area% of both iNOS and COX-2 immunostained sections of group II when compared to control. This was in accordance with Sakthivel and Guruvayoorappan, (2014) [56] and Barbosa Bezerra et al (2017) [18] . They reported that iNOS and COX-2 over expression was noted in AA induced colitis.…”

Myrtle berry seed aqueous extract inhibits human neutrophil myeloperoxidase in vitro and attenuates acetic acid-induced ulcerative colitis in rats

“…53,54,55 Indeed colonic inflammation is characterized by infiltration of neutrophils that migrate to the colonic wall after aggression. These data fully corroborated previous reports, indicating that acetic acid-induced colitis is accompanied by an oxidative balance perturbation.…”

“…These data fully corroborate previous reports, indicating that acetic acid-induced colitis is accompanied by an oxidative balance perturbation. [53][54][55] Indeed colonic inammation is characterized by the inltration of neutrophils that migrate to the colonic wall aer injury. 56 However the oxygen consumed by neutrophils is enzymatically converted to the superoxide anion (O 2 c À ) by the univalent transfer of two electrons from the cellular NADPH of the hexose monophosphate pathway.…”

Co-administration of α-lipoic acid and cyclosporine aggravates colon ulceration of acetic acid-induced ulcerative colitis via facilitation of NO/COX-2/miR-210 cascade

“…Conversely, excessive NO production associated with increased COX-2 exacerbates ulcerative colitis. In support of this notion, Sakthivel and Guruvayoorappan (2014) demonstrated that exacerbation of acetic acid-induced colitis in rats was accompanied by increased protein expression of NOS and COX-2. Notably, evidences showed that the positive or negative effects of NO on COX-2 expression depended on the microenvironment (Pérez-Sala and Lamas, 2001).…”