Modifying effect of vitamins C, E and beta-carotene against gamma-ray-induced DNA damage in mouse cells

Konopacka, Maria; Wideł, Maria; Rzeszowska-Wolny, Joanna

doi:10.1016/s1383-5718(98)00095-3

Cited by 134 publications

(86 citation statements)

References 21 publications

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“…Conversely co-treatment of cells with AA and RGS results in a statistically significant increase in both the cytotoxic and mutagenic effects of oxidative stress. Konopacka et al (1998) demonstrated the modifying effect of treatment with AA on the clastogenic activity of gamma rays in mice as measured by the micronucleus assay in bone marrow polychromatic erythrocytes and exfoliated bladder cells. Depending on AA concentration, the number of micronucleated polychromatic erythrocytes can be enhanced or reduced.…”

Section: Discussionmentioning

confidence: 95%

Modulatory effects of the antioxidant ascorbic acid on the direct genotoxicity of doxorubicin in somatic cells of Drosophila melanogaster

2007

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In this study two different crosses involving the wing cell markers mwh and flr 3 (standard (ST) cross and high bioactivation (HB) cross, the latter being characterized by a high constitutive level of cytochrome P450 which leads to an increased sensitivity to a number of promutagens and procarcinogens) were used to investigate the modulatory effects of ascorbic acid (AA) combined with the antitumor agent doxorubicin (DXR) in Drosophila melanogaster. We observed that the two different concentrations of AA (50 or 100 mM) had no effect on spots frequencies, while DXR treatments (0.2 or 0.4 mM) gave positive results for all types of spots, when compared to negative control. For marker-heterozygous (MH) flies, a protective effect was observed with the lower concentration of AA (50 mM) that was able to statistically decrease the frequency of spots induced by DXR (0.2 mM), while an enhanced frequency of spots induced by DXR was observed with the higher concentration of AA (100 mM), when compared to DXR treatment (p < 0.05). These results suggest that AA may interfere with free radicals generated by DXR and with other possible reactive metabolites. The efficiency of AA in protecting the somatic cells of D. melanogaster against mutation and recombination induced by DXR is dependent on the dose used and the protection is directly related to the activity of cytochrome P450 enzymes.

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Section: Discussionmentioning

confidence: 95%

Modulatory effects of the antioxidant ascorbic acid on the direct genotoxicity of doxorubicin in somatic cells of Drosophila melanogaster

2007

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“…Free radical scavenging has been studied for radioprotection of biologic materials [20,25,26]. Akkus et al [1] showed free radical scavengers can be partially successful in counteracting radiation effects in bone allografts.…”

Section: Discussionmentioning

confidence: 99%

“…Others have reported more successful radioprotection after combining radioprotective treatments [17,18]. A patented procedure known as the Clearant Process 1 (Clearant, Inc, Los Angeles, CA) reportedly provides full protection of musculoskeletal tissue using a scavenger cocktail and tightly regulated processing conditions, although it has not gained widespread acceptance [17,18], Also, Konopacka et al [20] and Packer et al [33] suggested vitamin combinations could act synergistically by regenerating molecular conformations capable of scavenging through free radical exchange. Our data suggest the combination of cross-linking and free radical scavengers has further improved protection against irradiation beyond that seen with single treatments [39].…”

Section: Discussionmentioning

confidence: 99%

Improved Tendon Radioprotection by Combined Cross-linking and Free Radical Scavenging

Seto

Gatt

Dunn

2009

Clinical Orthopaedics &Amp; Related Research

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Allograft safety is a great concern owing to the risk of disease transmission from nonsterile tissues. Radiation sterilization is not used routinely because of deleterious effects on the mechanical integrity and stability of allograft collagen. We previously reported several individual cross-linking or free radical scavenging treatments provided some radioprotective effects for tendons. We therefore asked whether a combination of treatments would provide an improved protective effect after radiation exposure regarding mechanical properties and enzyme resistance. To address this question we treated 90 rabbit Achilles tendons with a combination of cross-linking (1-ethyl-3-[3-dimethyl aminopropyl] carbodiimide [EDC]) and one of three scavenging regimens (mannitol, ascorbate, or riboflavin). Tendons then were exposed to one of three radiation conditions (gamma or electron beam irradiation at 50 kGy or unsterilized). Combination-treated tendons (10 per group) had increases in mechanical properties and higher resistance to collagenase digestion compared with EDC-only and untreated tendons. Irradiated tendons treated with EDC-mannitol, -ascorbate, and -riboflavin combinations had comparable strength to native tendon and had averages of 26%, 39%, and 37% greater, respectively, than those treated with EDC-only. Optimization of a crosslinking protocol and free radical scavenging cocktail is ongoing with the goal of ensuring sterile allografts through irradiation while maintaining their structure and mechanical properties.

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“…There are several types of tocopherol, α-tocopherol being more reactive and with stronger antioxidant power. It reacts quickly with peroxyl free radicals interrupting the free radical chain reaction and consequently protecting cells from damage (8). A wide range of radioprotective effects associated with vitamin E have been demonstrated, such as preservation of the small bowel crypt, increase in the rate of DNA repair process (8), salivary dysfunction (9), against mutagenic and/or carcinogenic agents in animals and cell cultures, and a reduction in the number of micronuclei in human lymphocytes in vitro (10).…”

Section: Introductionmentioning

confidence: 99%

Radioprotective Effect of Vitamin E in Parotid Glands: a Morphometric Analysis in Rats

et al. 2013

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The aim of this study was to evaluate the radioprotective effect of vitamin E on rat parotid glands by morphometric analysis. Sixty male rats were divided into 5 groups (n=6): control, in which animals received olive oil solution; olive oil/irradiated, in which animals received olive oil and were irradiated with a dose of 15 Gy of gamma radiation; irradiated, in which animals were irradiated with a dose of 15 Gy gamma radiation; vitamin E, which received α-tocopherol acetate solution; vitamin E/irradiated, which received α-tocopherol acetate solution before irradiation with a dose of 15 Gy gamma rays. Half of the animals were euthanized at 8 h, and the remaining at 30 days after irradiation. Both parotid glands were surgically removed and morphometric analysis of acinar cells was performed. Data were subjected to two-way ANOVA and Tukey's test (α=0.05). Morphometric analysis showed a significant reduction in the number of parotid acinar cells at 30 days in olive oil/irradiated and irradiated groups. In groups evaluated over time a significant reduction was shown at 30 days in olive oil/irradiated and irradiated groups, indicating that ionizing radiation caused tissue damage. The vitamin E/irradiated group presented more acinar cells than the irradiated group, but no statistically significant difference was observed (p>0.05). In conclusion, vitamin E seems to have failed as a radioprotective agent on acinar cells in rat parotid glands. R a d i o p r o t e c t i v e E f f e c t o f V i t a m i n E i n Pa r o t i d G l a n d s :

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Modifying effect of vitamins C, E and beta-carotene against gamma-ray-induced DNA damage in mouse cells

Cited by 134 publications

References 21 publications

Modulatory effects of the antioxidant ascorbic acid on the direct genotoxicity of doxorubicin in somatic cells of Drosophila melanogaster

Modulatory effects of the antioxidant ascorbic acid on the direct genotoxicity of doxorubicin in somatic cells of Drosophila melanogaster

Improved Tendon Radioprotection by Combined Cross-linking and Free Radical Scavenging

Radioprotective Effect of Vitamin E in Parotid Glands: a Morphometric Analysis in Rats

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