2007
DOI: 10.1590/s1415-47572007000300025
|View full text |Cite
|
Sign up to set email alerts
|

Modulatory effects of the antioxidant ascorbic acid on the direct genotoxicity of doxorubicin in somatic cells of Drosophila melanogaster

Abstract: In this study two different crosses involving the wing cell markers mwh and flr 3 (standard (ST) cross and high bioactivation (HB) cross, the latter being characterized by a high constitutive level of cytochrome P450 which leads to an increased sensitivity to a number of promutagens and procarcinogens) were used to investigate the modulatory effects of ascorbic acid (AA) combined with the antitumor agent doxorubicin (DXR) in Drosophila melanogaster. We observed that the two different concentrations of AA (50 o… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
2
1

Citation Types

5
15
0

Year Published

2008
2008
2023
2023

Publication Types

Select...
8

Relationship

1
7

Authors

Journals

citations
Cited by 23 publications
(20 citation statements)
references
References 48 publications
(53 reference statements)
5
15
0
Order By: Relevance
“…In addition to its mutagenic activity, DOX also has recombinogenic activity so that the frequency of twin spots reflected DOX-induced somatic recombination. These findings agree with other reports and show that DOX selectively induces homologous recombination when compared with mutational events in D. melanogaster somatic cells (Lehmann et al, 2003;Costa and Nepomuceno, 2006;Fragiorge et al, 2007;Valadares et al, 2008).…”
Section: Discussionsupporting
confidence: 93%
See 2 more Smart Citations
“…In addition to its mutagenic activity, DOX also has recombinogenic activity so that the frequency of twin spots reflected DOX-induced somatic recombination. These findings agree with other reports and show that DOX selectively induces homologous recombination when compared with mutational events in D. melanogaster somatic cells (Lehmann et al, 2003;Costa and Nepomuceno, 2006;Fragiorge et al, 2007;Valadares et al, 2008).…”
Section: Discussionsupporting
confidence: 93%
“…The main mechanisms of action proposed for DOX include the inhibition of topoisomerase II, DNA intercalation, free radical formation, reductive bioactivation of the quinine ring to a semiquinone radical, DNA alkylation and cross-linking (Gewirtz, 1999;Ramji et al, 2003;Navarro et al, 2006). These mechanisms can result in the cleavage of DNA which, if not repaired, may lead to mutations and chromosomal aberrations in tumors as well as in healthy cells (Antunes and Takahashi, 1998;Gentile et al, 1998;Islaih et al, 2005;Antunes et al, 2007;Costa and Nepomuceno, 2006;Fragiorge et al, 2007;Valadares et al, 2008).…”
Section: Introductionmentioning
confidence: 99%
See 1 more Smart Citation
“…Comparing the clone induction frequency in both genotypes obtained with DXR alone, we observed that 9.97% of the mutant clones produced in ST flies were a result of mutation and 90.03% of recombination, and that 17.89% of the spots induced in HB flies occurred due to mutation and 82.11% to recombination. The strong recombinogenic activity of DXR in somatic cells of D. melanogaster has been previously reported by Lehmann et al (2003), Costa and Nepomuceno (2006) and Fragiorge et al (2007). In ST cross descendants, recombination was responsible for reducing the frequency of spots produced by DXR in combination with 5, 10, and 15 mg/mL AEP by 68.14, 81.82, and 77.72%, respectively.…”
Section: Resultssupporting
confidence: 65%
“…melanogaster are versatile and sensitive in vivo eukaryotic systems for the determination of genotoxic and antigenotoxic activity of chemical compounds and complex mixtures (Graf et al, 1998;Idaomar et al, 2002;Silva et al, 2006;Fragiorge et al, 2007;Pantaleão et al, 2007;Téllez et al, 2007).…”
Section: Discussionmentioning
confidence: 99%