Radioprotective Effect of Vitamin E in Parotid Glands: a Morphometric Analysis in Rats

Abstract

The aim of this study was to evaluate the radioprotective effect of vitamin E on rat parotid glands by morphometric analysis. Sixty male rats were divided into 5 groups (n=6): control, in which animals received olive oil solution; olive oil/irradiated, in which animals received olive oil and were irradiated with a dose of 15 Gy of gamma radiation; irradiated, in which animals were irradiated with a dose of 15 Gy gamma radiation; vitamin E, which received α-tocopherol acetate solution; vitamin E/irradiated, which received α-tocopherol acetate solution before irradiation with a dose of 15 Gy gamma rays. Half of the animals were euthanized at 8 h, and the remaining at 30 days after irradiation. Both parotid glands were surgically removed and morphometric analysis of acinar cells was performed. Data were subjected to two-way ANOVA and Tukey's test (α=0.05). Morphometric analysis showed a significant reduction in the number of parotid acinar cells at 30 days in olive oil/irradiated and irradiated groups. In groups evaluated over time a significant reduction was shown at 30 days in olive oil/irradiated and irradiated groups, indicating that ionizing radiation caused tissue damage. The vitamin E/irradiated group presented more acinar cells than the irradiated group, but no statistically significant difference was observed (p>0.05). In conclusion, vitamin E seems to have failed as a radioprotective agent on acinar cells in rat parotid glands. R a d i o p r o t e c t i v e E f f e c t o f V i t a m i n E i n Pa r o t i d G l a n d s :

Comparative study of gamma radiation and prednisolone induced submandibular salivary gland oxidative damage

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Radiation Protection and Mitigation by Natural Antioxidants and Flavonoids: Implications to Radiotherapy and Radiation Disasters

“…In vivo studies have revealed that treatment with betacarotene is effective in amelioration of radiation toxicities in different tissues such as liver, thyroid gland, gastrointestinal system, bone marrow and blood [249][250][251][252][253]. However, for parotid glands, no radioprotective effect was observed [254]. These studies indicated that beta-carotene protects cells against ionizing radiation via scavenging of ROS and stimulation of SOD and catalase.…”

Capability of Vitamin E as A Radioprotector in Suppressing DNA Damage Determined with Comet Assay

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