Modification of the Genital Herpes Infection in a Guinea Pig Model, by Prior Immunization with Bovine Herpes Mammillitis Virus

Abstract

Bovine herpes mammillitis virus

Bovine herpesvirus 2 with low thymidine kinase activity and virulenc.

“…Routine motoring of potential recombinants by PCR using these primers can detect other low-DNA and low TK-producing TK isolates. We used these primers in PCR to distinguish BHV-2 or CSIRO290BU5 quickly from BHV-2 which can be isolated from mammary glands (5) and also from herpes simplex virus 1 and 2 which has close TK sequence homology with BHV-2 (8,18).…”

“…BHV-2 strain CSIRO 290 (22) was used in that study and used here also as it appears to be able to generate TK-deficient strains more easily than BHV-2 strain BHM-1 (10,25). Also, while CSIRO 290 results in only minimal symptoms in cattle (22,26) it does cause severe lesions in guinea pigs (8), allowing for testing of potential vaccines developed from that strain. This virus can be readily grown at 36°C in a variety of bovine cells including the established bovine cell line Madin-Darby bovine kidney (MDBK) cells (a bovine dirreahea virus-free line).…”

Isolation of a thymidine kinase‐deficient bovine herpesvirus 2 that still causes disease

“…When rested in the Hartley strain of guinea pigs (at 5 x 10 pfulanimal or higher; Maragos and May 1988;Orders et al 1995) C290BU3 produced disease similar to wild-type virus. This is in contrast to the two other TK-deficient strains so far reported (Smee and Leonhardt 1994;Orders eta1 1995).…”