Bovine herpesvirus 2 with low thymidine kinase activity and virulenc.

Development of a Shuttle Polymerase Chain Reaction for the Detection of Bovine Herpesvirus 2.

“…To our knowledge, there has been only one description of PCR used for monitoring thymidine kinase gene deficient BoHV-2 in attempts to produce a vaccine [4,10]; however, the method described showed a lower sensitivity than the methods under consideration here when examined in our laboratory.…”