Isolation of a thymidine kinase‐deficient bovine herpesvirus 2 that still causes disease

Development of a Shuttle Polymerase Chain Reaction for the Detection of Bovine Herpesvirus 2.

“…To our knowledge, there has been only one description of PCR used for monitoring thymidine kinase gene deficient BoHV-2 in attempts to produce a vaccine [4,10]; however, the method described showed a lower sensitivity than the methods under consideration here when examined in our laboratory.…”

“…Although Handley et al [4] determined the specificity of their PCR , its sensitivity was not described. In our experiment, the sensitivity of the PCR was examined according to the procedure described by Handley et al [4].…”

“…In our experiment, the sensitivity of the PCR was examined according to the procedure described by Handley et al [4]. Briefly, BoHV-2 DNA was amplified by 30 cycles of 92°C for 1 min, 57°C for 1 min, and 72°C for 30 sec using the primers 5' acg-cac-gaa-tgt-tgc-gtg 3' and 5' gct-cgc-tcc-cca-agt-ctg 3' (primer set A) or 5' cta-tca-cgt-ctg-gta-gcg-cg 3' and 5' tac-ttc-acg-gtg-ttg-gcg-ag 3' (primer set B).…”