Modification of Resolution in Capillary Electrophoresis for Protein Profiling in Identification of Genetic Modification in Foods

Latoszek, Anna; García-Ruiz, Carmen; Marina, Marı́a Luisa; Mata, F. Javier de la; Gómez, Rafael; Rasines, Beatriz; Cifuentes, Alejandro; Poboży, Ewa; Trojanowicz, Marek

doi:10.5562/cca1777

Cited by 8 publications

(4 citation statements)

References 37 publications

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“…Profiling analysis of proteins has been also reviewed for the analysis of genetically modified crops . CE‐UV was employed for protein profiling in extracts from transgenic and nontransgenic variants of maize and soybeans . Some protein changes were attributed to the genetic modification; however, it was indicated that larger number of samples might be needed to obtain statistically significant results.…”

Section: Peptides and Proteinsmentioning

confidence: 99%

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

et al. 2013

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In this work, the analysis of foods and food components using capillary electromigration methods is reviewed. The present work presents and discusses the main CE applications performed in Food Science and Technology including the new field of Foodomics, reviewing recent results on food quality and safety, nutritional value, storage, bioactivity, as well as applications of CE for monitoring food interactions and food processing. The CE analysis of a large variety of food-related molecules with different chemical properties, including amino acids, peptides, proteins, phenolic compounds, carbohydrates, DNA fragments, vitamins, toxins, pesticides, additives, and other minor compounds is described. The use of microchips, CE-MS, and chiral-CE in food analysis is also discussed as well as other current and foreseen trends in this area of research. Following the previous review by Castro-Puyana et al. (Electrophoresis, 2012, 33, 147–167), the current review covers the papers that were published from February 2011 to February 2013.

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Section: Peptides and Proteinsmentioning

confidence: 99%

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

et al. 2013

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“…In the case of the albumins, small quantitative and qualitative differences were found between both samples indicating differences in the content of the albumin fraction of the Bt11 maize in comparison with the isogenic counterpart. In another work, Latoszek et al proposed the use of dendrimers, symmetrical macromolecules with three-dimensional structures, as pseudostationary phases in CE to decrease protein adsorption and improve resolution on the protein profiles increasing the chance of discrimination between conventional and transgenic maize [ 39 ]. Silicon-based and dendrimers with amine core were tested in different concentrations.…”

Section: Applications Of Capillary Electrophoresis (Ce) To the Stumentioning

confidence: 99%

Characterization and Study of Transgenic Cultivars by Capillary and Microchip Electrophoresis

Vega

Marina

2014

IJMS

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Advances in biotechnology have increased the demand for suitable analytical techniques for the analysis of genetically modified organisms. Study of the substantial equivalence, discrimination between transgenic and non-transgenic cultivars, study of the unintended effects caused by a genetic modification or their response to diverse situations or stress conditions (e.g., environmental, climatic, infections) are some of the concerns that need to be addressed. Capillary electrophoresis (CE) is emerging as an alternative to conventional techniques for the study and characterization of genetically modified organisms. This article reviews the most recent applications of CE for the analysis and characterization of transgenic cultivars in the last five years. Different strategies have been described depending on the level analyzed (DNA, proteins or metabolites). Capillary gel electrophoresis (CGE) has shown to be particularly useful for the analysis of DNA fragments amplified by PCR. Metabolites and proteins have been mainly separated using capillary zone electrophoresis (CZE) using UV and MS detection. Electrophoretic chips have also proven their ability in the analysis of transgenic cultivars and a section describing the new applications is also included.

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“…To date, a wide variety of methodologies have been developed for the monitoring of GM contents, such as two-dimensional electrophoresis (Kim et al 2006 ), protein capillary electrophoresis (Latoszek et al 2011 ), HPLC (López et al 2009 ), and ELISA (Xu et al 2009 ). Of these methodologies though, polymerase chain reaction (PCR) and real-time PCR is currently the most widely applied technique owing to its high sensitivity and specificity (Marmiroli et al 2008 ) while conventional PCR methods need to handle post-PCR products for gel electrophoresis or enzymatic digestion, real-time PCR does not need post-PCR manipulations which significantly reduce the risk of laboratory contamination and is more convenient.…”

Section: Introductionmentioning

confidence: 99%

Development of a qualitative real-time PCR method to detect 19 targets for identification of genetically modified organisms

Peng

Wang

et al. 2016

SpringerPlus

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As the amount of commercially available genetically modified organisms (GMOs) grows recent years, the diversity of target sequences for molecular detection techniques are eagerly needed. Considered as the gold standard for GMO analysis, the real-time PCR technology was optimized to produce a high-throughput GMO screening method. With this method we can detect 19 transgenic targets. The specificity of the assays was demonstrated to be 100 % by the specific amplification of DNA derived from reference material from 20 genetically modified crops and 4 non modified crops. Furthermore, most assays showed a very sensitive detection, reaching the limit of ten copies. The 19 assays are the most frequently used genetic elements present in GM crops and theoretically enable the screening of the known GMO described in Chinese markets. Easy to use, fast and cost efficient, this method approach fits the purpose of GMO testing laboratories.

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Modification of Resolution in Capillary Electrophoresis for Protein Profiling in Identification of Genetic Modification in Foods

Cited by 8 publications

References 37 publications

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

Characterization and Study of Transgenic Cultivars by Capillary and Microchip Electrophoresis

Development of a qualitative real-time PCR method to detect 19 targets for identification of genetically modified organisms

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