Characterization and Study of Transgenic Cultivars  by Capillary and Microchip Electrophoresis

Vega, Elena Domínguez; Marina, Marı́a Luisa

doi:10.3390/ijms151223851

Cited by 16 publications

(7 citation statements)

References 52 publications

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“…CE is a versatile technique that has found a huge number of applications in food analysis and Foodomics , as can be also corroborated from the information given in Table . Thus, due to its high separation efficiency, extremely small sample and reagents volume requirements, and rapid analysis, CE has demonstrated to be a very useful analytical tool in food science.…”

Section: Introductionmentioning

confidence: 72%

“…MCE has continued demonstrating its potential for in situ analysis and fast multiparameter analysis. In the last years, novel developments and application of MCE have kept growing and several reviews have been published focusing on the application of this technology to the field of nutrition , food analysis , as well as to other specific food issues such as the analysis of transgenic food . In addition, the use of multidimensional carbon allotropes as electrochemical detectors in MCE has been recently reviewed by Martín et al.…”

Section: Microchip Ce (Mce) Technology In Food Analysismentioning

confidence: 99%

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Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

et al. 2015

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This review work presents and discusses the main applications of capillary electromigration methods in food analysis and Foodomics. Papers that were published during the period February 2013-February 2015 are included following the previous review by Garcia-Cañas et al. (Electrophoresis, 2014, 35, 147-169). Analysis by CE of a large variety of food-related molecules with different chemical properties, including amino acids, hazardous amines, peptides, proteins, phenols, polyphenols, lipids, carbohydrates, DNAs, vitamins, toxins, contaminants, pesticides, residues, food additives, as well as small organic and inorganic compounds. This work includes recent results on food quality and safety, nutritional value, storage, bioactivity, as well as applications of CE for monitoring food processing. The use, among other CE developments, of microchips, CE-MS, and chiral CE in food analysis and Foodomics is also discussed.

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Section: Introductionmentioning

confidence: 72%

Section: Microchip Ce (Mce) Technology In Food Analysismentioning

confidence: 99%

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

et al. 2015

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“…The technique is mainly designed for the transgenic event detection from GM corn. As compared to the electrophoresis gel, CGE system has higher resolution power to clearly detect PCR products from a multiplex assay ( Vega and Marina, 2014 ). Moreover, a single5-plex (pentaplex) PCR and double 6-plex (hexaplex) PCR have also been designed specially to identify different numbers of events from GM corn and cotton ( Nadal et al, 2009 ; Holck and Pedersen, 2011 ).…”

Section: Capillary Gel Electrophoresis (Cge)mentioning

confidence: 99%

Molecular Approaches for High Throughput Detection and Quantification of Genetically Modified Crops: A Review

et al. 2017

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As long as the genetically modified crops are gaining attention globally, their proper approval and commercialization need accurate and reliable diagnostic methods for the transgenic content. These diagnostic techniques are mainly divided into two major groups, i.e., identification of transgenic (1) DNA and (2) proteins from GMOs and their products. Conventional methods such as PCR (polymerase chain reaction) and enzyme-linked immunosorbent assay (ELISA) were routinely employed for DNA and protein based quantification respectively. Although, these Techniques (PCR and ELISA) are considered as significantly convenient and productive, but there is need for more advance technologies that allow for high throughput detection and the quantification of GM event as the production of more complex GMO is increasing day by day. Therefore, recent approaches like microarray, capillary gel electrophoresis, digital PCR and next generation sequencing are more promising due to their accuracy and precise detection of transgenic contents. The present article is a brief comparative study of all such detection techniques on the basis of their advent, feasibility, accuracy, and cost effectiveness. However, these emerging technologies have a lot to do with detection of a specific event, contamination of different events and determination of fusion as well as stacked gene protein are the critical issues to be addressed in future.

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“…In a second step, the PCR products are analyzed using the CGE, microarray, or Luminex platforms. Despite the fact that these technologies present a higher throughput than qPCR, their multiplexing level is still influenced by the inherent properties of PCR which limit the number of reactions at commonly ten targets per PCR assay [ 133 , 134 ].…”

Section: Gmo Detection Approachesmentioning

confidence: 99%

“…In order to detect simultaneously several targets, the use of the PCR multiplex CGE, where fluorescently labelled primers allow discriminating different amplicons of the same size, has been also suggested to be applied in the GMO detection field ( Figure 1 and Table 4 ). Compared to the electrophoresis gel, the resolution power of the CGE system to detect PCR products from a multiplex assay is clearly higher [ 134 ]. However, the sensitivity of CGE system is weaker than the qPCR technology [ 135 ].…”

Section: Gmo Detection Approachesmentioning

confidence: 99%

Current and New Approaches in GMO Detection: Challenges and Solutions

Fraiture

Herman

Taverniers³

et al. 2015

BioMed Research International

116

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In many countries, genetically modified organisms (GMO) legislations have been established in order to guarantee the traceability of food/feed products on the market and to protect the consumer freedom of choice. Therefore, several GMO detection strategies, mainly based on DNA, have been developed to implement these legislations. Due to its numerous advantages, the quantitative PCR (qPCR) is the method of choice for the enforcement laboratories in GMO routine analysis. However, given the increasing number and diversity of GMO developed and put on the market around the world, some technical hurdles could be encountered with the qPCR technology, mainly owing to its inherent properties. To address these challenges, alternative GMO detection methods have been developed, allowing faster detections of single GM target (e.g., loop-mediated isothermal amplification), simultaneous detections of multiple GM targets (e.g., PCR capillary gel electrophoresis, microarray, and Luminex), more accurate quantification of GM targets (e.g., digital PCR), or characterization of partially known (e.g., DNA walking and Next Generation Sequencing (NGS)) or unknown (e.g., NGS) GMO. The benefits and drawbacks of these methods are discussed in this review.

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Characterization and Study of Transgenic Cultivars by Capillary and Microchip Electrophoresis

Cited by 16 publications

References 52 publications

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

Molecular Approaches for High Throughput Detection and Quantification of Genetically Modified Crops: A Review

Current and New Approaches in GMO Detection: Challenges and Solutions

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