Modification of reduced graphene/Au-aptamer to develop an electrochemical based aptasensor for measurement of glycated albumin

Farzadfard, Azad; Shayeh, Javad Shabani; Habibi-Rezaei, Mehran; Omidi, Meysam

doi:10.1016/j.talanta.2020.120722

Cited by 52 publications

(28 citation statements)

References 35 publications

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“…Several studies about the electrochemical biosensor for GA measurement have been reported using enzymes, antibody, aptamer or peptide as the biorecognition molecules and based on amperometry, electrochemiluminescence (ECL), impedance, voltammetry or field effect transistor (FET) as sensing principles [7,[16][17][18][19][20][21][22][23]. These studied are summarized in Table 1.…”

Section: Measurement Of Fructosyl Lysine and Ga With An Ide Enzyme Sementioning

confidence: 99%

Development of an Interdigitated Electrode-Based Disposable Enzyme Sensor Strip for Glycated Albumin Measurement

et al. 2021

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Glycated albumin (GA) is an important glycemic control marker for diabetes mellitus. This study aimed to develop a highly sensitive disposable enzyme sensor strip for GA measurement by using an interdigitated electrode (IDE) as an electrode platform. The superior characteristics of IDE were demonstrated using one microelectrode of the IDE pair as the working electrode (WE) and the other as the counter electrode, and by measuring ferrocyanide/ferricyanide redox couple. The oxidation current was immediately reached at the steady state when the oxidation potential was applied to the WE. Then, an IDE enzyme sensor strip for GA measurement was prepared. The measurement of fructosyl lysine, the protease digestion product of GA, exhibited a high, steady current immediately after potential application, revealing the highly reproducible measurement. The sensitivity (2.8 nA µM−1) and the limit of detection (1.2 µM) obtained with IDE enzyme sensor strip were superior compared with our previously reported sensor using screen printed electrode. Two GA samples, 15 or 30% GA, corresponding to healthy and diabetic levels, respectively, were measured after protease digestion with high resolution. This study demonstrated that the application of an IDE will realize the development of highly sensitive disposable-type amperometric enzyme sensors with high reproducibility.

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Section: Measurement Of Fructosyl Lysine and Ga With An Ide Enzyme Sementioning

confidence: 99%

Development of an Interdigitated Electrode-Based Disposable Enzyme Sensor Strip for Glycated Albumin Measurement

et al. 2021

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“…Consequently, by incorporating these nucleic acid aptamers into various sensing platforms, it is possible to subject these probes to repeated use without loss of functionality, thereby allowing a device to be potentially recyclable. Numerous aptamer-based approaches have been reported for chemical and biological detection, such as surface-enhanced Raman spectroscopy [40,41], SPR [42,43], fluorescence [44,45], colorimetry [46,47], and electrochemistry [48,49].…”

Section: Introductionmentioning

confidence: 99%

Recent Advancements in Aptamer-Based Surface Plasmon Resonance Biosensing Strategies

Chang

2021

Biosensors

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Surface plasmon resonance (SPR) can track molecular interactions in real time, and is a powerful as well as widely used biological and chemical sensing technique. Among the different SPR-based sensing applications, aptamer-based SPR biosensors have attracted significant attention because of their simplicity, feasibility, and low cost for target detection. Continuous developments in SPR aptasensing research have led to the emergence of abundant technical and design concepts. To understand the recent advances in SPR for biosensing, this paper reviews SPR-based research from the last seven years based on different sensing-type strategies and sub-directions. The characteristics of various SPR-based applications are introduced. We hope that this review will guide the development of SPR aptamer sensors for healthcare.

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“…Additionally, the antibodies used in biosensors could be of weak stability, low operability, and high cost. Aptamer (Apt), an oligonucleotide for detecting targets through directed self-assembly (Zhou et al, 2017;Hao et al, 2019), has ideal properties and is more modifiable in various recognition molecules for monitoring bacteria (Duan et al, 2020;Farzadfard et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

A novel smartphone-based colorimetric aptasensor for on-site detection of Escherichia coli O157:H7 in milk

Yang

Wang

Yang

et al. 2021

Journal of Dairy Science

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Effective testing tools for Escherichia coli O157:H7 can prevent outbreaks of foodborne illness. In this paper, a smartphone-based colorimetric aptasensor was developed using functionalized gold nanoparticles (GNP) and multi-walled carbon nanotubes (MWCNT) for monitoring E. coli O157:H7 in milk. The maximum absorption peak of GNP bonded with aptamer (Apt) generated evident transformation from 518 to 524 nm. The excess GNP-Apt was removed by functionalized MWCNT magnetized with carbonyl iron powder (CIP) and hybridized with a DNA probe, whereas the GNP-Apt immobilized on E. coli O157:H7 remained in the system. In the presence of a high-salt solution, the GNP-Apt that captured E. coli O157:H7 remained red, but the free GNP-Apt aggregated and appeared blue. The chromogenic results were analyzed by a smartphone-based colorimetric device that was fabricated using acrylic plates, a light-emitting diode, and a mobile power pack. To our knowledge, this was the first attempt to use a smartphone-based colorimetric aptasensor employing the capture of GNP-Apt coupled with separation of MWCNT@ CIP probe to detect E. coli O157:H7. The aptasensor exhibited good reproducibility and no cross-reaction for other bacteria. A concentration of 8.43 × 10 3 cfu/mL of E. coli O157:H7 could be tested in pure culture, and 5.24 × 10 2 cfu/ mL of E. coli O157:H7 could be detected in artificially contaminated milk after 1 h of incubation. Therefore, the smartphone-based colorimetric aptasensor was an efficient tool for the detection of E. coli O157:H7 in milk.

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Modification of reduced graphene/Au-aptamer to develop an electrochemical based aptasensor for measurement of glycated albumin

Cited by 52 publications

References 35 publications

Development of an Interdigitated Electrode-Based Disposable Enzyme Sensor Strip for Glycated Albumin Measurement

Development of an Interdigitated Electrode-Based Disposable Enzyme Sensor Strip for Glycated Albumin Measurement

Recent Advancements in Aptamer-Based Surface Plasmon Resonance Biosensing Strategies

A novel smartphone-based colorimetric aptasensor for on-site detection of Escherichia coli O157:H7 in milk

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