Recent Advancements in Aptamer-Based Surface Plasmon Resonance Biosensing Strategies

Chang, Chia Chen

doi:10.3390/bios11070233

Cited by 41 publications

(22 citation statements)

References 136 publications

(160 reference statements)

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“…Surface plasmon resonance (SPR) is a well-known sensing technology used for characterising kinetics of ligand–receptor interactions and these types of sensors offer unique real-time and label-free measurement capabilities with high detection sensitivity [ 44 , 45 , 46 ]. Figure 2 A shows a typical SPR biosensor configuration where biorecognition elements, such as antibodies, are immobilised on the surface of a thin gold film.…”

Section: Latest Developed Biosensors For Covid-19mentioning

confidence: 99%

Emerging Biosensors to Detect Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2): A Review

2021

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Coronavirus disease (COVID-19) is a global health crisis caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) is the gold standard test for diagnosing COVID-19. Although it is highly accurate, this lab test requires highly-trained personnel and the turn-around time is long. Rapid and inexpensive immuno-diagnostic tests (antigen or antibody test) are available, but these point of care (POC) tests are not as accurate as the RT-PCR test. Biosensors are promising alternatives to these rapid POC tests. Here we review three types of recently developed biosensors for SARS-CoV-2 detection: surface plasmon resonance (SPR)-based, electrochemical and field-effect transistor (FET)-based biosensors. We explain the sensing principles and discuss the advantages and limitations of these sensors. The accuracies of these sensors need to be improved before they could be translated into POC devices for commercial use. We suggest potential biorecognition elements with highly selective target-analyte binding that could be explored to increase the true negative detection rate. To increase the true positive detection rate, we suggest two-dimensional materials and nanomaterials that could be used to modify the sensor surface to increase the sensitivity of the sensor.

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Section: Latest Developed Biosensors For Covid-19mentioning

confidence: 99%

Emerging Biosensors to Detect Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2): A Review

2021

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“…Third, aptamers are smaller, facilitating their immobilization on surfaces, providing more target binding sites while minimizing steric hindrance effects. Consequently, the aptamer‐bound target is located closer to the transducing surface, which can be advantageous for increasing the biosensing signal [ 92 ], for example, in surface Plasmon resonance (SPR) biosensors [ 93 , 94 ]. Aptamers are also advantageous for the detection of small molecules and ions when compared to antibodies [ 95 ], owing to their capability to undergo significant conformational changes upon target binding, leading to higher signal and sensitivity.…”

Section: Aptasensorsmentioning

confidence: 99%

Aptasensors versus immunosensors—Which will prevail?

Arshavsky‐Graham

Heuer

Xin

et al. 2022

Engineering in Life Sciences

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Since the invention of the first biosensors 70 years ago, they have turned into valuable and versatile tools for various applications, ranging from disease diagnosis to environmental monitoring. Traditionally, antibodies have been employed as the capture probes in most biosensors, owing to their innate ability to bind their target with high affinity and specificity, and are still considered as the gold standard. Yet, the resulting immunosensors often suffer from considerable limitations, which are mainly ascribed to the antibody size, conjugation chemistry, stability, and costs. Over the past decade, aptamers have emerged as promising alternative capture probes presenting some advantages over existing constraints of immunosensors, as well as new biosensing concepts. Herein, we review the employment of antibodies and aptamers as capture probes in biosensing platforms, addressing the main aspects of biosensor design and mechanism. We also aim to compare both capture probe classes from theoretical and experimental perspectives. Yet, we highlight that such comparisons are not straightforward, and these two families of capture probes should not be necessarily perceived as competing but rather as complementary. We, thus, elaborate on their combined use in hybrid biosensing schemes benefiting from the advantages of each biorecognition element.

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“…Compared to antibodies, aptamers show numerous advantages, such as high stability, sensitivity, low cost of synthesis and easy modification process. Because of these properties, the use of aptamers has increased exponentially in the design of biosensors in the last decade [49] , [50] , [51] , [52] .…”

Section: Electrochemical Biosensors For Covid-19mentioning

confidence: 99%

Electrochemical biosensors for SARS-CoV-2 detection: Voltametric or impedimetric transduction?

Antiochia

2022

Bioelectrochemistry

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Recent Advancements in Aptamer-Based Surface Plasmon Resonance Biosensing Strategies

Cited by 41 publications

References 136 publications

Emerging Biosensors to Detect Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2): A Review

Emerging Biosensors to Detect Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2): A Review

Aptasensors versus immunosensors—Which will prevail?

Electrochemical biosensors for SARS-CoV-2 detection: Voltametric or impedimetric transduction?

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