Modification of polysulfones by click chemistry: Amphiphilic graft copolymers and their protein adsorption and cell adhesion properties

Abstract

Well‐defined amphiphilic graft copolymer with hydrophobic polysulfone (PSU) backbone and hydrophilic poly(acrylic acid) (PAA) side chains were synthesized and characterized. For this purpose, commercially available PSU was converted to azido‐functionalized polymer (PSU‐N3) by successive chloromethylation and azidation processes. Independently, poly(tert‐butyl acrylate) (PtBA) with an alkyne‐end‐group is obtained by using suitable initiator in atom transfer radical polymerization (ATRP). Then, this polymer was successfully grafted onto PSU‐N3 by click chemistry to yield polysulfone‐graft‐poly(tert‐butyl acrylate), (PSU‐g‐PtBA). Finally, amphiphilic polysulfone‐graft‐poly(acrylic acid), (PSU‐g‐PAA), membranes were obtained by hydrolyzing precursor the PSU‐g‐PtBA membranes in trifluoroacetic acid. The final polymer and intermediates at various stages were characterized by 1H NMR, FTIR, GPC, and SEM analyses. Protein adsorption and eukaryotic and prokaryotic cell adhesion on PSU‐g‐PAA were studied and compared to those of PSU‐g‐PtBA and unmodified PSU. © 2010 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2010

Grafting of Poly (Cysteine Methacrylate) Brush from Polysulfone Membrane via Surface-Initiated ATRP and Their Anti-Protein Fouling Property

“…Cysteine and 3-(acryloyloxy)-2-hydroxypropyl methacrylate were purchased from Aladdin Reagent (Shanghai) Co., Ltd. and used without purification. Chloromethylation of PSU was performed according to the reported methods [22]. Cysteine Methacrylate monomer (CysMA) was synthesized according to the published work [16].…”

Synthesis, characterization, and hydrolytic degradation of graft copolymers of polystyrene and aliphatic polyesters

“…[33,34] Many examples of click reactions involving macromolecules were reported. In this laboratory, we have focused on the use of such click reactions for the synthesis of various macromolecular architectures [34][35][36][37][38][39][40][41] and functionalization of polymers [42][43][44][45][46][47], microspheres [48], clays [49,50], and silsesquioxanes [51] with thermal-, photo-, and electro-active groups.…”

Polysulfone/Pyrene Membranes: A New Microwell Assay Platform for Bioapplications

“…[9] In recent years, the main scientific and applied interest is focused not only on the synthesis of new types of polymeric materials but also on the modification of existing polymers to alter their properties to meet requirements for new applications. [10] As a fluorescence probe, pyrene (Py) has been attractive because of its high fluorescence yield, stability and characteristic excimer formation. Particular attention has been directed on the integration of Py units into polymers.…”