Polysulfone/Pyrene Membranes: A New Microwell Assay Platform for Bioapplications

Abstract

The use of PSU-Py prepared by click chemistry as a platform in membrane-bottom microwell plates for oxidase and hydrolase/oxidase-based enzyme assays is studied. For the GOx assay, the postulated fluorescence mechanism is based on the consumption of glucose by dissolved oxygen and GOx in the microwell plates covered with the PSU-Py membrane. For the AG-GOx assay, maltose is used as AG substrate and hydrolyzed to glucose which is then oxidized by the GOx activity. It is shown that the PSU-Py membrane acts as a fluorescence indicator of the enzymatic reactions, and both GOx and AG/GOx enzyme assays are successfully applied for glucose, maltose and acorbose analysis in the range 0.125-2.0 × 10(-3) M glucose, 0.05-0.5 × 10(-3) M maltose, and 0.0125-0.1 mg · mL(-1) acorbose, respectively.

Recent advances in the preparation of functionalized polysulfones

“…Parallel to this, terminal alkyne‐functionalized polymers were synthesized by ATRP which provided control of the functional group at the chain end. These polymers were subsequently grafted onto the polysulfone backbone through the highly efficient and selective ‘click’ reaction . Protein adsorption and cell adhesion studies show that the obtained amphiphilic graft copolymers (polysulfone‐ g ‐poly(acrylic acid) and polysulfone‐ g ‐poly(ethylene oxide)) exhibited bioinert characteristics (Scheme ).…”

“…These polymers were subsequently grafted onto the polysulfone backbone through the highly efficient and selective 'click' reaction. 254,255 Protein adsorption and cell adhesion studies show that the obtained amphiphilic graft copolymers (polysulfone-g-poly(acrylic acid) 256 and polysulfoneg-poly(ethylene oxide) 257 ) exhibited bioinert characteristics (Scheme 15). In a recent study, the grafting of phosphonated poly(pentafluorostyrene) onto a polysulfone backbone is also performed via the 'click' chemistry approach.…”

Visible light‐induced synthesis of polysulfone‐based graft copolymers by a grafting from approach

“…Besides, amphiphilic PSU graft copolymers were also obtained by the hydrolyzation in the case of t BA containing graft copolymer. The obtained amphiphilic graft copolymers would be promising candidates for various practices especially biomedical applications such as bioinert membranes …”

Synthesis of Polysulfone Based Amphiphilic Graft Copolymers by a ‘Grafting to’ Approach

“…In the first step of the procedure, pendant chloride moieties were incorporated into PSU to yield a functional PSU (PSU-Cl) that can be used as macroinitiator in conjunction with Mn 2 (CO) 10 . 36,37 Besides, amphiphilic PSU graft copolymers were also obtained by the hydrolyzation in the case of tBA containing graft copolymer.…”