Modification of oestrogen-induced uterine hyperaemia by drugs in the ovariectomized rat

1991

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1 The thromboxane receptor antagonists EP092, AH23848 and BM 13.505 were used to investigate the role of thromboxane in the uterotrophic response to oestradiol and tamoxifen. 2 The parameters examined were uterine blood flow (measured by the microsphere technique), uterine wet and dry weights and the concentrations of cytosolic and nuclear oestrogen receptors. 3 Only EP092 potentiated the hyperaemic response to oestrogen but all three thromboxane antagonists inhibited oestradiol-stimulated uterine growth. This inhibition was accompanied by a decrease in nuclear oestrogen receptor concentration. 4 The uterotrophic response to tamoxifen was unaffected by the thromboxane antagonists. 5 The mechanism by which the thromboxane antagonists may be exerting their growth inhibitory effect is discussed, although, whether this effect can be attributed to blockade of thromboxane receptors or to some other mechanism is not clear from this study.

Section: Measurement Of Bloodflowmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Modification of the rat uterine response to oestrogen and tamoxifen by thromboxane antagonists

Kerr

1991

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“…The microsphere technique (previously described by Phaily & Senior, 1978a) Marshall & Senior (1987). Briefly, animals were killed by cervical dislocation and the uteri were rapidly removed and homogenized in PE buffer (10mM phosphate buffer, pH 7.4, containing 1.5 mm EDTA and 2 mM mercaptoethanol).…”

Section: Measurement Of Bloodflowmentioning

confidence: 99%

The effects of thromboxane receptor antagonists on oestrogen‐induced uterotrophic responses in the spontaneously hypertensive rat

Kerr

1991

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1 The possible role of thromboxane in the uterotrophic response to oestrogen, in the spontaneously hypertensive rat was investigated by use of the thromboxane receptor antagonists EP092, AH23848 and BM 13.505.2 The parameters studied were uterine blood flow (measured by the microsphere technique), uterine wet and dry weights and the concentrations of cytosolic and nuclear oestrogen receptors. 3 The antagonists attenuated oestradiol-induced uterine blood flow and significantly reduced both wet and dry uterine weight. These changes were accompanied by decreases in nuclear oestrogen receptor levels. 4 The results suggest a supportive role for thromboxane in oestradiol-induced uterine growth.

“…Blood flow was measured in rats, anaesthetized with sodium pentobarbitone (60mg kg-'), by the micro-sphere technique described in previous work (Phaily & Senior, 1978 …”

Section: Measurement Of Bloodflowmentioning

confidence: 99%

The effect of mepyramine and ranitidine on the oestrogen and anti‐oestrogen stimulated rat uterus

1986

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1 The aims of this study were to elucidate further the role of histamine in the rat uterotrophic response and to investigate the differences between the oestrogen and the anti-oestrogen induced uterine responses. 2 The parameters examined were uterine blood flow (measured by the microsphere technique), uterine wet and dry weights.3 17P-Oestradiol and the anti-oestrogen, tamoxifen, were used to stimulate the ovariectomised rat uterus and the antihistamines mepyramine (HI) and ranitidine (H2) were employed to modify these responses.4 The uterine changes evoked by oestradiol proved to be more susceptible to modification by the antihistamines than the tamoxifen-stimulated responses. 5 The results suggest that histamine is involved in the early uterine response to oestradiol but histamine does not appear to play a major role in the uterine response to tamoxifen.