Modification of a nitrocellulose membrane with cellulose nanofibers for enhanced sensitivity of lateral flow assays: application to the determination of Staphylococcus aureus

A Cellulose Paper-Based Fluorescent Lateral Flow Immunoassay for the Quantitative Detection of Cardiac Troponin I

“…A couple more recent studies also demonstrate how LFA sensitivity to protein and nucleic detection can be enhanced by layering cellulose nanofibers (CNF) on the test line regions of NC strips [ 22 , 23 ]. The core idea is to promote the penetration of CNF into the top pores of NC and, thus, contribute to increase the amount of capture antibodies close to the surface of the strips.…”

“…The core idea is to promote the penetration of CNF into the top pores of NC and, thus, contribute to increase the amount of capture antibodies close to the surface of the strips. As a result, the density of selectively bound gold nanoparticles in the top part of test lines increases, enhancing the LFA sensitivity by 36.6% in the case of the detection of human IgG [ 22 ] and by 20-fold in the case of the detection of Staphylococcus aureus nucleic acid [ 23 ].…”

Recent advances in cellulose-based membranes for their sensing applications

“…b The structure illustration of LFAs test strips before and after CNFs modification; c The modification process of CNFs on NC membrane; d Schematic illustration of LFAs test strip detection. Adapted with permission from Tang et al ( 2019 ), Copyright 2019, Springer …”

“…Staphylococcus aureus , as a common clinical pathogen, can cause skin purulent infection, pneumonia, pericarditis, sepsis and other diseases (Tong et al 2015 ). Our group reported the lateral flow assays based on nitrocellulose membranes with cellulose nanofibers for the determination of Staphylococcus aureus (Tang et al 2019 ) (Fig. 9 b–d).…”

“…The raw materials for cellulose membranes preparation can include ethyl cellulose, cellulose nanofibers, cellulose nanocrystals, cellulose acetate, triacetyl cellulose, sodium carboxymethyl cellulose, nitrocellulose, etc. (Fu et al 2019;Zhu et al 2019a, b;Li et al 2017a, b, c;Amin 2015;Duong et al 2018;Hittini et al 2020;Tang et al 2019). The excellent sensing performances can be obtained by introducing metal nanowires, metal nanoparticles, carbon dots, quantum dots, carbon nanotubes, fluorophores, organic dyes and antigen-antibodies into cellulose membranes through physical-chemical methods (Lou et al 2020;Zhang et al 2019a, b, c;Jiang et al 2020a, b;Li et al 2017a, b, c;Lv et al 2019a, b;Shankar et al 2020).…”