Models in Ophthalmology and Vision Research

Optimization and Validation of a High‐Performance Liquid Chromatography (HPLC‐UV) Method for Quantification of α‐Lipoic Acid in Cyclodextrins Complex for Ocular Delivery

“…The results showed that more ALA passed through the sclera than the cornea. The rabbit cornea's thickness is approximately 400 μm while the sclera's thickness can vary between 180 and 250 μm, which could partly support the theory that ALA penetrated faster through the sclera than the cornea (Peiffer et al 1994). We predict that in human eyes, ALA would follow the same pathway, because the human cornea is thicker than the human sclera at the site of administration (Camburu et al 2023;Vurgese et al 2012).…”

Optimization and Validation of a High‐Performance Liquid Chromatography (HPLC‐UV) Method for Quantification of α‐Lipoic Acid in Cyclodextrins Complex for Ocular Delivery

“…The results showed that more ALA passed through the sclera than the cornea. The rabbit cornea's thickness is approximately 400 μm while the sclera's thickness can vary between 180 and 250 μm, which could partly support the theory that ALA penetrated faster through the sclera than the cornea (Peiffer et al 1994). We predict that in human eyes, ALA would follow the same pathway, because the human cornea is thicker than the human sclera at the site of administration (Camburu et al 2023;Vurgese et al 2012).…”

Animal Models for the Study of Keratoconus

“…Despite presenting with some of the histological characteristics of KC, this model does not exhibit localized central thinning, stromal thinning, or gross signs of KC. The study notes changes to Bowman's layer; however, this layer is not present in the rabbit cornea (Table 2) [61,62]. The loss of collagen fiber parallelism and structural integrity identified in their H&E staining as occurring in the Bowman's layer may actually refer to the stroma, which would be consistent with KC [1,46].…”

Enhanced fabrication of dual-compartment artificial cornea C-Clear via precision moulding and continuous polymerisation: biocompatibility and functional efficacy in rabbit model

“…The human central cornea is significantly thicker (approximately 550 µm) than that of the rabbit (approximately 400 µm). 78 Critically, the rabbit cornea lacks a distinct Bowman's layer, an acellular membrane that provides structural integrity in the human cornea. The absence of this layer in rabbits leads to a fundamentally different wound healing process, characterized by more rapid epithelial regeneration and a reduced tendency for stromal scarring compared to humans, in whom damage to Bowman's layer is irreversible and often results in fibrosis.…”

Optimization and Validation of a High‐Performance Liquid Chromatography (HPLC‐UV) Method for Quantification of α‐Lipoic Acid in Cyclodextrins Complex for Ocular Delivery

“…The results showed that more ALA passed through the sclera than the cornea. The rabbit cornea's thickness is approximately 400 μm while the sclera's thickness can vary between 180 and 250 μm, which could partly support the theory that ALA penetrated faster through the sclera than the cornea (Peiffer et al 1994). We predict that in human eyes, ALA would follow the same pathway, because the human cornea is thicker than the human sclera at the site of administration (Camburu et al 2023;Vurgese et al 2012).…”

Animal Models for the Study of Keratoconus

“…Despite presenting with some of the histological characteristics of KC, this model does not exhibit localized central thinning, stromal thinning, or gross signs of KC. The study notes changes to Bowman's layer; however, this layer is not present in the rabbit cornea (Table 2) [61,62]. The loss of collagen fiber parallelism and structural integrity identified in their H&E staining as occurring in the Bowman's layer may actually refer to the stroma, which would be consistent with KC [1,46].…”

Enhanced fabrication of dual-compartment artificial cornea C-Clear via precision moulding and continuous polymerisation: biocompatibility and functional efficacy in rabbit model

“…The human central cornea is significantly thicker (approximately 550 µm) than that of the rabbit (approximately 400 µm). 78 Critically, the rabbit cornea lacks a distinct Bowman's layer, an acellular membrane that provides structural integrity in the human cornea. The absence of this layer in rabbits leads to a fundamentally different wound healing process, characterized by more rapid epithelial regeneration and a reduced tendency for stromal scarring compared to humans, in whom damage to Bowman's layer is irreversible and often results in fibrosis.…”

Optimization and Validation of a High‐Performance Liquid Chromatography (HPLC‐UV) Method for Quantification of α‐Lipoic Acid in Cyclodextrins Complex for Ocular Delivery

“…The results showed that more ALA passed through the sclera than the cornea. The rabbit cornea's thickness is approximately 400 μm while the sclera's thickness can vary between 180 and 250 μm, which could partly support the theory that ALA penetrated faster through the sclera than the cornea (Peiffer et al 1994). We predict that in human eyes, ALA would follow the same pathway, because the human cornea is thicker than the human sclera at the site of administration (Camburu et al 2023;Vurgese et al 2012).…”

Animal Models for the Study of Keratoconus

“…Despite presenting with some of the histological characteristics of KC, this model does not exhibit localized central thinning, stromal thinning, or gross signs of KC. The study notes changes to Bowman's layer; however, this layer is not present in the rabbit cornea (Table 2) [61,62]. The loss of collagen fiber parallelism and structural integrity identified in their H&E staining as occurring in the Bowman's layer may actually refer to the stroma, which would be consistent with KC [1,46].…”

Enhanced fabrication of dual-compartment artificial cornea C-Clear via precision moulding and continuous polymerisation: biocompatibility and functional efficacy in rabbit model

“…The human central cornea is significantly thicker (approximately 550 µm) than that of the rabbit (approximately 400 µm). 78 Critically, the rabbit cornea lacks a distinct Bowman's layer, an acellular membrane that provides structural integrity in the human cornea. The absence of this layer in rabbits leads to a fundamentally different wound healing process, characterized by more rapid epithelial regeneration and a reduced tendency for stromal scarring compared to humans, in whom damage to Bowman's layer is irreversible and often results in fibrosis.…”