Modelling and predicting the interactions between oppositely and variously charged polyelectrolytes by frontal analysis continuous capillary electrophoresis

Lounis, Feriel Meriem; Chamieh, Joseph; Leclercq, Laurent; Gonzalez, Philippe; Cottet, Hervé

doi:10.1039/c6sm01811d

Cited by 17 publications

(35 citation statements)

References 59 publications

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“…The ionic strength did not significantly influence the binding stoichiometry whatever the PAMAMPS chemical charge density and the DGL generation number. This result is similar to what was observed in a previous work for linear PLL [41,42,43]. The fluctuations in stoichiometry were attributed to experimental errors.…”

Section: Resultssupporting

confidence: 91%

“…Isotherms of adsorption were plotted by representing the average number of bound ligands (DGL) per substrate molecule (PAMAMPS) truen¯ (calculated according to Equation (2)) vs. the free ligand concentration [ DGL ] for different initial molar ratio [ DGL ] 0 /[ PAMAMPS ] 0 . The stoichiometry of interaction n expressed in term of bound DGL entities per PAMAMPS chain and the binding site constant k were determined by non-linear least square routine on Microsoft excel using the model of identical interacting sites [42] expressed by Equation (3).…”

Section: Resultsmentioning

confidence: 99%

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Effect of Dendrigraft Generation on the Interaction between Anionic Polyelectrolytes and Dendrigraft Poly(l-Lysine)

et al. 2018

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Abstract:In this present work, three generations of dendrigraft poly(L-Lysine) (DGL) were studied regarding their ability to interact with linear poly (acrylamide-co-2-acrylamido-2-methyl-1-propanesulfonate) (PAMAMPS) of different chemical charge densities (30% and 100%). Frontal analysis continuous capillary electrophoresis (FACCE) was successfully applied to determine binding constants and binding stoichiometries. The effect of DGL generation on the interaction was evaluated for the first three generations (G2, G3, and G4) at different ionic strengths, and the effect of ligand topology (linear PLL vs. dendrigraft DGL) on binding parameters was evaluated. An increase of the biding site constants accompanied with a decrease of the DGL-PAMAMPS (n:1) stoichiometry was observed for increasing DGL generation. The logarithm of the global binding constants decreased linearly with the logarithm of the ionic strength. This double logarithmic representation allowed determining the extent of counter-ions released from the association of DGL molecules onto one PAMAMPS chain that was compared to the total entropic reservoir constituted by the total number of condensed counter-ions before the association.

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Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Effect of Dendrigraft Generation on the Interaction between Anionic Polyelectrolytes and Dendrigraft Poly(l-Lysine)

et al. 2018

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“…Hence, ACE possesses a great potential for investigation of the (bio)molecular noncovalent interactions under mild conditions and for estimation of binding (stability, association, formation) or dissociation constants of the formed complexes as demonstrated in several recent reviews and research articles . ACE involves several modes based either on the separation of the interacting species and determination of their equilibrium concentration, such as in the Hummel–Dreyer method , the vacancy peak method , frontal analysis , continuous frontal analysis , and kinetic CE , or on the detection of a specific physicochemical property of the complexed ligand or the binding partner, mostly the changes of effective mobility in the mobility shift assay or changes of migration times in the partial‐filling ACE . All these methods utilize the differences in the migration velocities of the interacting species.…”

Section: Separations By the Particular Ce And Cec Methodsmentioning

confidence: 99%

“…The interactions between oppositely and variously charged polyelectrolytes, cationic poly(‐L‐lysine) (PLL) and variously charged statistical copolymers of acrylamide and 2‐acrylamido‐2‐methyl‐1‐propanesulfonate (PAMAMPS) with charge densities 15–100% were studied by frontal analysis continuous CE (FACCE) . FACCE performed in poly(diallyl dimethyl ammonium chloride) coated capillary in the high ionic strength BGE (12 mM Tris, 10 mM HCl, 305–990 mM NaCl, pH 7.4) enabled determination of binding constants and stoichiometry of the formed complexes.…”

Section: Applicationsmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Kašička

2017

Electrophoresis

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The review brings a comprehensive overview of recent developments and applications of high performance capillary and microchip electroseparation methods (zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) to analysis, microscale isolation, purification, and physicochemical and biochemical characterization of peptides in the years 2015, 2016, and ca. up to the middle of 2017. Advances in the investigation of electromigration properties of peptides and in the methodology of their analysis (sample preseparation, preconcentration and derivatization, adsorption suppression and EOF control, and detection) are described. New developments in particular CE and CEC methods are presented and several types of their applications to peptide analysis are reported: qualitative and quantitative analysis, determination in complex (bio)matrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid, sequence and chiral analysis, and peptide mapping of proteins. Some micropreparative peptide separations are shown and capabilities of CE and CEC methods to provide important physicochemical characteristics of peptides are demonstrated.

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“…The binding constants of complexes can be determined by several physico‐chemical methods, for example, by isothermal titration calorimetry , equilibrium dialysis , NMR or fluorescence spectrometry , MS , HPLC , and PAGE . In addition, several CE methods are available for determination of the binding constants of complexes, such as mobility shift ACE (ms‐ACE) , Hummel‐Dreyer (HD) method , mobility shift vacancy ACE , frontal analysis CE , continuous frontal analysis CE , kinetic CE and partial filling ACE (PF‐ACE) . The principles and comparison of these methods have been thoroughly discussed in several review articles .…”

Section: Introductionmentioning

confidence: 99%

Determination of binding constants of multiple charged cyclodextrin complexes by ACE using uncorrected and ionic strength corrected actual mobilities of the species involved

et al. 2019

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Determination of binding constants of multiple charged cyclodextrin complexes by ACE using uncorrected and ionic strength corrected actual mobilities of the species involvedIn this study, the apparent binding constants and limiting mobilities of the multiply charged complexes of the − and −enantiomers of Ru(II)-and Fe(II)-polypyridyl associates ([Ru(2,2 -bipyridine) 3 ] 2+ , [Ru(1,10-phenanthroline) 3 ] 2+ , and [Fe(1,10phenanthroline) 3 ] 2+ ) with single-isomer 2,3-diacetylated-6-sulfated-cyclodextrins (CDs) (12Ac-6S-α-CD, 14Ac-7S-β-CD, and 16Ac-8S-γ-CD) were determined by ACE using uncorrected and ionic strength corrected actual mobilities of the species involved. Two limiting models were tested for the ionic strength correction of the actual mobilities based on an empirical relation for the ionic strength correction of multivalent ionic species. In model 1, the nominal values of the charge numbers (z S,nom ) and analytical concentrations (c S,nom ) of the above CD selectors in the BGEs were applied for calculation of the BGE ionic strength, as usual. In model 2, the CD selectors were considered as singly charged species (z S = −1) with |z S,nom |-times higher concentrations in the BGE than their analytical concentrations (c S = |z S,nom | × c S,nom ) in the calculation of the BGE ionic strength. In all three cases-with uncorrected actual mobilities as well as with actual mobilities corrected according to the two limiting models-the measured effective mobilities of the above enantiomers fit well the theoretical curves of their mobility dependences on the CD selectors concentrations in the BGE, with high average coefficients of determination (R 2 = 0.9890-0.9995). Nevertheless, the best physico-chemically meaningful values of the apparent binding constants and the limiting mobilities of the enantiomer-CDs complexes with low RSDs were obtained using the actual mobilities of the species involved corrected according to model 2.

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Modelling and predicting the interactions between oppositely and variously charged polyelectrolytes by frontal analysis continuous capillary electrophoresis

Cited by 17 publications

References 59 publications

Effect of Dendrigraft Generation on the Interaction between Anionic Polyelectrolytes and Dendrigraft Poly(l-Lysine)

Effect of Dendrigraft Generation on the Interaction between Anionic Polyelectrolytes and Dendrigraft Poly(l-Lysine)

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Determination of binding constants of multiple charged cyclodextrin complexes by ACE using uncorrected and ionic strength corrected actual mobilities of the species involved

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