Effect of Dendrigraft Generation on the Interaction between Anionic Polyelectrolytes and Dendrigraft Poly(l-Lysine)

Lounis, Feriel Meriem; Chamieh, Joseph; Leclercq, Laurent; Gonzalez, Philippe; Rossi, Jean‐Christophe; Cottet, Hervé

doi:10.3390/polym10010045

Cited by 11 publications

(12 citation statements)

References 55 publications

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“…The interactions between oppositely and variously charged polyelectrolytes, cationic poly(‐L‐lysine) (PLL) and variously charged statistical copolymers of acrylamide and 2‐acrylamido‐2‐methyl‐1‐propanesulfonate (PAMAMPS) with charge densities 15–100% were studied by frontal analysis continuous CE (FACCE) . FACCE performed in poly(diallyl dimethyl ammonium chloride) coated capillary in the high ionic strength BGE (12 mM Tris, 10 mM HCl, 305–990 mM NaCl, pH 7.4) enabled determination of binding constants and stoichiometry of the formed complexes.…”

Section: Applicationsmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Kašička

2017

Electrophoresis

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The review brings a comprehensive overview of recent developments and applications of high performance capillary and microchip electroseparation methods (zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) to analysis, microscale isolation, purification, and physicochemical and biochemical characterization of peptides in the years 2015, 2016, and ca. up to the middle of 2017. Advances in the investigation of electromigration properties of peptides and in the methodology of their analysis (sample preseparation, preconcentration and derivatization, adsorption suppression and EOF control, and detection) are described. New developments in particular CE and CEC methods are presented and several types of their applications to peptide analysis are reported: qualitative and quantitative analysis, determination in complex (bio)matrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid, sequence and chiral analysis, and peptide mapping of proteins. Some micropreparative peptide separations are shown and capabilities of CE and CEC methods to provide important physicochemical characteristics of peptides are demonstrated.

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Section: Applicationsmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Kašička

2017

Electrophoresis

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“…Equation (3a) was used repeatedly to model the interaction, and in many cases a good linearity was found, at least at higher ionic strength (see the discussion of this problem in Ref. [109]) Recently, a careful investigation of this problem was presented by Lounis et al., [164, 165] who demonstrated that Equation (3a) provides an excellent description of experimental data for the interaction of linear and dendrigraft poly(lysine) with synthetic anionic polyelectrolytes.…”

Section: Fundamentals and Linear Polyelectrolytesmentioning

confidence: 99%

Understanding the Interaction of Polyelectrolyte Architectures with Proteins and Biosystems

et al. 2020

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The counterions neutralizing the charges on polyelectrolytes such as DNA or heparin may dissociate in water and greatly influence the interaction of such polyelectrolytes with biomolecules, particularly proteins. In this Review we give an overview of studies on the interaction of proteins with polyelectrolytes and how this knowledge can be used for medical applications. Counterion release was identified as the main driving force for the binding of proteins to polyelectrolytes: Patches of positive charge become multivalent counterions of the polyelectrolyte and lead to the release of counterions from the polyelectrolyte and a concomitant increase in entropy. This is shown from investigations on the interaction of proteins with natural and synthetic polyelectrolytes. Special emphasis is paid to sulfated dendritic polyglycerols (dPGS). The Review demonstrates that we are moving to a better understanding of charge–charge interactions in systems of biological relevance. Research along these lines will aid and promote the design of synthetic polyelectrolytes for medical applications.

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“…92 Colcom, a French start-up, has commercialized a dendrimer-like structure first discovered by Collet et al and known as "lysine dendrigrafts," which display primary amine functionalities along its backbone and surface ( Figure 11). [77][78][79][80][81][82][83][84][85][86][87][88][89][90][91][92][94][95][96][97][98][99][100][101][102][103][104][105][106][127][128][129][130][131][132][133][134][135][136][137][138] Lysine dendrigrafts are primarily utilized for drug 89 and gene delivery. 107 but have also been investigated for their potential as a coagulant, 108 imaging agent, 95 and substrates for tissue engineering.…”

Section: Hydrophilic Ifdsmentioning

confidence: 99%

Synthesis, structure, and function of internally functionalized dendrimers

Smith

Gorman

Menegatti

2020

Journal of Polymer Science

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The past three decades have witnessed an exponential increase in the structural diversity and applications of dendrimers, spanning across drug delivery and diagnostics, protein, and enzyme mimicry, solubility enhancement, coatings, light harvesting, and catalysis. The dendrimer community has recently focused on internally functionalized dendrimers (IFDs) owing to their advanced design and functionality. The synthesis of IFDs relies on advanced orthogonal chemistries and/or (de)protection schemes, as well as careful purification to minimize polydispersity of composition and molecular weight. The studies published on IFDs, however, lay scattered across the chemical literature, and a comprehensive presentation of structural rationale, synthetic procedures, and technologically relevant applications is missing. To address this need, this review presents a comprehensive collection and discussion of all available studies on IFDs, detailing their methods of synthesis and their structure-function correlations. The wide variety of internal functionalities, including hydroxyl, amine, carboxylic acid, allyl, alkyne, and imidazole groups, enables myriad applications in biochemistry, chemical and biomedical engineering, and material science. Particular focus is given to IFDs that are amenable to modular synthetic strategies, which promote higher synthetic yield and scalability, and therefore possess stronger translational and commercial potential. As such, this review guides research groups pursuing the difficult task of IFD rational design and synthesis providing them a concise roadmap to their mission.

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Effect of Dendrigraft Generation on the Interaction between Anionic Polyelectrolytes and Dendrigraft Poly(l-Lysine)

Cited by 11 publications

References 55 publications

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Understanding the Interaction of Polyelectrolyte Architectures with Proteins and Biosystems

Synthesis, structure, and function of internally functionalized dendrimers

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